adipose tissue suggested increased content of milk fat and changed milk fatty acid composition, and also caused decreased milk protein content ( 6 , 25 ). Several authors reported that ketosis decreases both milk lactose yield and lactose percentage ( 4 ), and high availability of blood glucose and a cow’s positive metabolic energy status are always translated into high milk lactose yield and lactose percentage ( 18 ). A high milk fat : protein ratio is considered to be a signifier of a high risk of ketosis in dairy cattle ( 2 ). Research described cut-off points for
transformation rates. In general, liming promotes N fixation by the soil microbial community ( Holland et al., 2018 ). The effects on N mineralization are less clear-cut. N mineralization has been reported to increase, decrease, or remain unchanged ( Bailey et al 1995 ; Kemmitt et al., 2006 ; Wachendorf et al., 2015 ) but probably ameliorates with repeated lime applications ( Holland et al., 2018 ); however, if augmented, also availability for plant uptake increases and crop yields can benefit correspondingly, but N losses via nitrate leaching into groundwater can be spurred
I. Christiana, R. Aparnaa, R. Rohit, D. Nageswara Rao and P. Kaliraj
periodically after immunization and stored at -80 °C and the antibody titer was determined by ELISA, in which 96-well microtiter plates (Immulon2, Dynatech, VA, USA) were coated with 100 ng/well of respective antigen. The plates were blocked with 5 % skimmed milk, after washing with PBST and PBS, a 2 fold serial dilution from 1:500 to 1:64000 were performed using respective antisera. The antibody titer was determined by fixing a cut-off value which was obtained by the mean plus 3SD of the OD value of pre-immune serum. The highest dilution of the antiserum that showed an OD
Ali Hajimohammadi, Khalil Badiei, Parviz Kheibari, Meherdad Pourjafar and Aliasghar Chalmeh
the preservation of peroxidase activity of haemoglobin procedure, which is directly proportional to the amount of Hp (Tridelta Development Ltd., Ireland). The lowest measuring interval of this test has been determined as 15.6 mg/L by the manufacturer. Tumour necrosis factor-α (TNF-α) was measured in serum by a solid phase sandwich ELISA (product no. AbC 606 Eurobio AbCys S.A., France). For TNF-α, a cut-off point of 129 ng/L represented 96% sensitivity and 93% specificity. Measurement of lactate was performed in plasma taken from potassium oxalate/sodium fluoride
]; Z. Untersuch. Lebensm. 74 (1937) 408–411.
92. Haag, H.B.: Chemical and pharmacologic observations on nicotine and tobacco smoke; The Merck Report (October, 1940) pp. 25–29.
93. Shmuk, A.A. and N. Piatnicki: Acids of tobacco. II; J. Assoc. Off. Agr. Chem. 69 (1930) 19–26, see Chem. Abstr. 25 (1931) 3124.
94. Wada, E. and Y. Kobashi: Chemical constituents of tobacco. IV. Determination of organic acids in tobacco leaves and isolation of organic acids by paper chromatography; J. Agr. Chem