Professional training of future US border guard officers at the Federal Law Enforcement Training Center using e-FLETC Online Campus has been substantiated in the article. Special attention has been paid to revealing such topical areas of Online Campus computer-based training modules (crime scene, driver training, drugs, firearms, health, interviewing, investigative, legal, mapping, maritime, officer safety, technology, terrorism, traffic stops, training) that include over 120 lessons and 20 videos which are available on a wide range of topics. Web-based training lessons which include topics such as counterterrorism, crime scene preservation and documentation, disaster, crisis and emergency strategies and management, domestic violence, drug endangered children, drug related crimes, elder abuse, firearms, fitness and healthy lifestyles, human trafficking, interacting with special needs populations, intelligence led policing, interpersonal skills and conflict management, interviews and interrogations, investigating technology related crimes (protecting and collecting digital evidence), investigative skills and techniques, knowledge of laws and regulations, leadership and management, lessons learned (previous case analysis), maritime law enforcement, etc. have been revealed. Besides, examples of “Firearms” and “Use of Force” curricula in e-FLETC Online Campus have been presented.