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Open access

Tomasz Stankiewicz and Barbara Błaszczyk

Abstract

The objective of the study was to determine the concentration of BMP-15 and GDF-9 in the fluid of follicular cysts and ovarian follicles, and to compare their concentrations in mono- and polyoocyte follicles in gilts. The study involved two experiments conducted on the ovaries collected post-slaughter from gilts (7-8 months old). The first experiment covered 31 follicular single cyst gilts (15-25 mm in diameter) and 41 gilts without cysts. Follicular fluid from follicles of 8-10 mm in diameter (n=41) and 5-8 mm in diameter (n=41), and cystic fluid (n=31) were collected for analysis. The second experiment involved collecting follicular fluid from poly- (n=19) and monooocyte (n=22) follicles. The concentration of BMP-15 and GDF-9 was then determined in the samples using specimen-specific ELISA kits. The differences in the concentration of these factors were calculated by means of analysis of variance and a posthoc test. Duncan’s multiple range test was used to verify the significance of differences at P<0.05 and P<0.01. In addition, correlations between the factors were calculated. BMP-15 and GDF-9 levels in the cystic fluid were significantly higher than those in the follicular fluid (P<0.01). However, no differences were observed between various size follicles or between mono- and polyoocyte follicles. BMP-15 and GDF-9 concentrations were found to be positively correlated (P<0.01). Differences in BMP-15 and GDF-9 concentrations in ovarian follicles and follicular cysts, as evidenced by our study, indicate that these factors may be related to folliculogenesis disorders in gilts. What is more, the number of oocytes in ovarian follicles does not influence the intrafollicular concentration of BMP-15 and GDF-9.

Open access

Verica Mrvić, Rizah Avdić, Nedžad Hadžiomerović, Faruk Tandir, Branislav Prokić, Ratko Tomašević and Bogomir Bolka Prokić

: Circulation of blood through the canine kidney. Amer J Vet Res 1952. 13:236-245. 25. Kürtül I, Dursun N, Özcan S: Relation of arterial vascularization of the kidney and adrenal gland of the german shepherd dogs. J Fac Vet Med Univ Istanbul 2002, 28:65-71. 26. Raj JU, Hillyard R, Kaapa P, Anderson J, Gropper M: Pulmonary vascular pressure profile in 2-3-week-old,5-6-week-old and adult ferrets. Respir physiol 1990, 82:307-315. 27. Sampaio FJ, Aragao AH: Anatomical relationship between the intrarenal arteries and the kidney collecting system. J Urol 1990, 143

Open access

Branimira Špoljarić, Maja Popović, Josip Crnjac, Zrinka Žderić Savatović, Martina Ratko, Mateja Lozančić, Matea Jurak, Igor Špoljarić, Daniel Špoljarić and Gordan Mršić

, Cheung BKK: Double swab technique for collecting touched evidence. Legal Med 2007, 9:181-184.

Open access

Dorota Grygoruk

Abstract

The development of information technology makes it possible to collect and analyse more and more data resources. The results of research, regardless of the discipline, constitute one of main sources of data. Currently, the research results are increasingly being published in the Open Access model. The Open Access concept has been accepted and recommended worldwide by many institutions financing and implementing research. Initially, the idea of openness concerned only the results of research and scientific publications; at present, more attention is paid to the problem of sharing scientific data, including raw data. Proceedings towards open data are intricate, as data specificity requires the development of an appropriate legal, technical and organizational model, followed by the implementation of data management policies at both the institutional and national levels. The aim of this publication was to present the development of the open data concept in the context of open access idea and problems related to defining data in the process of data sharing and data management.

Open access

Mehrdad Ghodskhah Daryayi, Mohammad Naghi Adel, Mohaddese Seddighi Pashaki and Javad Sadegh Kuhestani

Abstract

Fire usually causes changes in the composition and diversity of herbaceous species. The present paper aimed to study the effect of repeated fire incidents on understory species diversity in the Saravan forests of Guilan Province located in the north of Iran. To do so, three 50-hectare areas with identical physiographical conditions and overstory (hand-planted softwood Pinus taeda) were selected. Seven fire incidences occurred for the 10-year period in one of the areas and the other area experienced three fire incidents within the same period. The area with no fire incidents was considered as the control area. All the fire incidents were surface ones. The systematic random method with a sample size 100 × 200 m was used for collecting data. Based on the above, 25 samples were determined in each area. The whole coverage of the understory was taken into consideration using Whittaker’s method and the Domain criterion so as to calculate species diversity indices. The results showed that Shannon-Wiener diversity and Menhinick’s richness in the regions which experienced 3 and 7 fire incidents were maximum and minimum, respectively, whereas no significant difference was seen between the regions with regard to Smith and Wilson’s evenness index.

Open access

Hieronim Borowicz, Krzysztof Kubiak, Goran Nikolovski and Artur Niedźwiedź

Abstract

The aim of this review was to collect the most important data on the characteristics of selected antioxidants in horses with recurrent airway obstruction (RAO). RAO is one of the most common respiratory diseases in horses. This disease can not be cured, but we can try to stop its progression. It is not exactly known, what is the real contribution of antioxidants in the pathology of recurrent airway obstruction. Many researchers investigated the possibility of using antioxidant supplementation in horses with RAO.

Open access

Hristo Daskalov, Fejzulla Fejzullah and Alexandra Daskalova

Abstract

The aim of this study was to collect actual data concerning the frequency of contamination with Listeria monocytogenes of some raw dried cured vacuum packed sausages, which are very popular in Bulgaria produced from Oct. 2004 till May 2008. 148 vacuum-packed samples were taken from 9 different food business operators during all seasons of the year. The samples were analyzed according to the USDA method for meat foods. Ten specimens were positive for presence of Listeria monocytogenes, which is equal to 6,75% of all tested samples. In two other raw dried cured sausages L. welshimeri and L.innocua were found, but these species are not pathogenic for consumers. In the period before the official implementation of the HACCP system (01.01.2006) in Bulgaria, 52 samples were examined and 5 Listeria monocytogenes isolates were found (~10%). 2,5 years after the HACCP implementation, 96 specimens from the same meat factories were tested and 5 Listeria monocytogenes isolates (5,2%) were detected. Samples taken from lots, produced in winter time were contaminated with Listeria monocytogenes more often (7 of all 10) than specimens taken during other seasons. Data was discussed through the point of view of the effectiveness of hygienic practices and HACCP system application. Also, application of the ‘microbiological criterion’ set in the COMMISSION REGULATION (EC) No 2073/2005 for ready-to-eat foods unable to support the growth of L. monocytogenes was considered.

Open access

A. Dors, E. Czyżewska, M. Pomorska-Mól, R. Kołacz and Z. Pejsak

Abstract

The aim of this study was to collect production data of Polish swine herds, with special emphasis on the production parameters in farrow to finish pig herds. Another goal was to determine differences in the production performance of swine herds with different sizes, various status with regard to biosecurity, and with different veterinary expenditure. For this purpose, questionnaire surveys were carried out in 96 polish farrow to finish pig herds. The data concerning production parameters (e.g. the number of pigs born per sow per year, litters per sow per year, pre- and post-weaning mortality), farm size (small, medium, large), management (all in-all out by room or building), veterinary expenditure (including medication) and the percentage of pigs under medical treatment, were collected.

The data obtained in the present study indicate that in general, the efficiency of swine production in evaluated farms was relatively low. It was also found that in large swine farms the efficiency was better than that in small ones and that the proper biosecurity positively influenced the performance of the swine farms. However, only in 10.4% facilities, the biosecurity rules and methods, including the principle “all-in all-out”, were implemented and kept. It seems that inefficient swine production on the majority of Polish farms results from poor basic knowledge on pig production and understanding of fundamental economic rules of swine breeding.

Open access

Ilona Bachanek, Marcin Barszcz, Marcin Taciak, Anna Tuśnio and Jacek Skomiał

Abstract

The aim of the study was to evaluate the effects of dietary level of two types of inulin differing in the degree of polymerization (DP) on microbial activity in the large intestine of chicks. The experiment was performed on 70 one-day-old Ross 308 male chicks divided into 7 groups fed starter-type diets without inulin addition or supplemented with 0.2%, 0.4% or 0.6% of inulin with DP≥10 (IN10), or DP≥23 (IN23). After 14 days of feeding birds were sacrificed to collect digesta samples from caeca and colon. Caecal digesta was examined for pH, concentrations of short-chain fatty acids (SCFA) and amines, activities of β-glucosidase and mucinase, and relative amount of selected bacterial populations, whereas in colonic digesta only pH, SCFA and amines were analysed. Regardless of DP, inulin level did not affect digesta pH, activity of bacterial enzymes and relative amounts of Clostridium spp., Lactobacillus spp., Bifidobacterium spp. and E. coli populations. Dietary level of IN10 significantly affected propionic acid concentration, which was greater in caecal digesta of birds fed diet supplemented with 0.2% of IN10 compared to other groups and feeding diets supplemented with all levels of IN10 significantly reduced histamine concentration compared to the control. There were no effects of inulin on microbial activity indices in the colon. The present study indicates that, regardless of DP, inulin does not modify considerably microbial activity in the large intestine of chicks.

Open access

G. Daniel-Igwe and N. Okwara

Abstract

One hundred (50 males and 50 females) B-not strain indigenous turkeys, Meleagris gallopavo, were used to determine the reference values for their haematological parameters. The turkeys were housed in the poultry unit and jugular venepunctures were used to collect their blood. The haematological parameters were determined using standard procedures. The mean values of: the packed cell volume (PCV; 37.29 ± 0.37 %), red blood cell (RBC) counts (2.50 ± 0.44 × 106.µl−1), haemoglobin concentration (Hbc; 10.89 ± 0.34 g.dl−1), mean corpuscular volume (MCV; 150.63 ± 0.73 fl), mean corpuscular haemoglobin (MCH; 44.29 ± 1.78 pg), mean corpuscular haemoglobin concentration (MCHC; 29.10 ± 0.73 g.dl−1), and white blood cell (WBC) counts (12.41 ± 0.83 × 103 µl−1) were determined. No significant differences were found between the male and female B-not strain turkeys in this study. The results will help in the interpretation of cases of disease when there are variations in the values and serve as baseline data for B-not strain of turkeys in the humid tropics.