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F. Zigo, M. Vasiľ, L. Takáč, M. Zigová and J. Elečko

: Pheno- and genotyping of Staphylococcus epidermidis isolated from bovine milk and human skin. Vet. Microbiol. , 115, 163—172. 19. Vautor, E., Cocckfield, J., Marechal, C., Loir, Y., Le Chevalier, M., Robinson, A. D., et al., 2009: Difference in virulence between Staphylococcus aureus isolates causing gangrenous mastitis versus subclinical mastitis in a dairy sheep flock. V et. Res. , 6, 40—56. 20. Vršková, M., Tančin, V., Kirchnerová, K., Sláma, P., 2015: Evaluation of daily milk production in Tsigaia ewes by somatic cell count. Potravinárstvo

Open access

Kamila Myszka and Katarzyna Czaczyk

lactobacilli alter their adhesion to human intestinal mucus glycoproteins. Int. J. Food Microbiol., 2000, 60, 75-81. van Houdt R., Michiels C. W., Role of bacterial cell surface structures in Escherichia coli biofilm formation. Res. Microbiol., 2005, 156, 626-633. Vuong C., Otto M., Staphylococcus epidermidis infections. Microb. Infection, 2002, 4, 481-489. Wirtanen G., Matilla-Sandholm T., Epifluorescence image analysis and cultivation of foodborne biofilm bacteria grown on stainless steel

Open access

Alīna Klūga, Margarita Terentjeva, Attila Kántor, Maciej Kluz, Czeslaw Puchalski and Miroslava Kačániová

Abstract

The aim of the present study was to detect the antibacterial activity of medicinal plants against fish microflora. A total of 4 ethanolic extracts of 6 plant species were collected from local environments of Slovakia and screened for antibacterial activity against bacterial microflora. Extracts of Melissa officinalis L., Mentha piperita L., Origanum vulgare L. and Malva mauritiana were used. Bacterial strains were isolated from common bleak (Alburnus alburnus) and common rudd (Scardinius erythrophthalmus) of Latvian origin. All bacterial strains were identified with the Matrix- Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS). Among fish microflora, Acinetobacter pittii, A. baumannii, Buttiauxella agrestis, Delftia acidovorans, Enterobacter cloacae, Serratia liquefaciens, Pseudomonas alcaligenes, Ps. oryzihabitans, Staphylococcus epidermidis, St. caprae, Pantoea agglomerans, Lelliottia amnigena, Providencia rettgeri, Escherichia coli and Rahnella aquatilis were identified. It has been shown that all plant extracts exhibit different degrees of antimicrobial activity against the tested bacteria. All bacterial strains in the present study were moderate sensitive to all extracts applied. The strongest antimicrobial effect of Malva mauritiana and Melissa officinalis L. against Pseudomonas oryzihabitans (6.67±1.53 resp. 9.67±0.58 mm) were found. The best antimicrobial activity of Mentha piperita L. was against Staphylococcus epidermis (7.33±0.58 mm) and strongest antimicrobial effect of Origanum vulgare L. was same against two bacterial strains Enterobacter cloacae and Serratia liquefaciens (9.67±0.58 mm).

Open access

A. Piegerová, J. Koščová, P. Schusterová, R. Nemcová and M. Kryvtsova

-017-0580-9. 11. Kumar, A., Alam, A., Rani, M., Ehtesham, N. Z., Hasnain, S. E., 2017: Biofilms: Survival and defense strategy for pathogens. Int. J. Med. Microb. , 307 (8), 481 — 489. DOI: 10.1016/j.ijmm.2017.09.016. 12. Nogueira, J., Costa, R., da Cunha, M., Cavalcante, T., 2017: Antibiofilm activity of natural substances derived from plants. African Journal of Microbiology Research , 11 (26), 1051 — 1060. DOI: 10.5897/AJMR2016.8180. 13. O’Gara, J. P., 2007: ica and beyond: biofilm mechanism and regulation of Staphylococcus epidermidis and Staphylococcus

Open access

Basel Saleh, Lila Al-Halab and Ayman Al-Mariri

antimicrobial activity of Psidium guajava Linn. leaf. Nature Sci 2010; 8(12):43-50. 11. Dhiman A, Nanda A, Ahmad S, Narasimhan B. In vitro antimicrobial activity of methanolic leaf extract of Psidium guajava L. J Pharm Bioall Sci 2011; 3:226-229. 12. Fagbohun RT, Joshua AT, Philips AJ. Effect of aqueous extract of leaf and bark of guava ( Psidium guajava ) on fungi Microsporum gypseum and Trichophyton mentagrophytes , and bacteria Staphylococcus aureus and Staphylococcus epidermidis. Adv Med Plant Res 2013; 1(2):45-48. 13. Shruthi SD, Roshan A

Open access

Wiesław Piotr Świderek, Krystyna Małgorzata Charon, Anna Winnicka, Joanna Gruszczyńska and Mariusz Pierzchała

A., Kluciński W., Hoser G., Sikora J., Kawiak J. (1999). Flow cytometry analysis of milk and peripheral blood cells from goats during lactation. J. Vet. Med., 46: 459-464. Winnicka A., Kluciński W., Kawiak J., Hoser G., Sikora J. (2000). Effect of Baypamun on blood leucocytes in normal and dexamethasone treated goats. J. Vet. Med., 47: 385-394. Winter P., Colditz I.G. (2002). Immunological responses of the lactating ovine udder following experimental challenge with Staphylococcus epidermidis. Vet. Immunol. Immunopathol., 89: 57-65.

Open access

Olga Kosakowska and Weronika Czupa

. Bocchini P, Russo M, Galletti GC. Pyrolysis gas-chromatography/mass spectrometry used as a microanalytical technique for the characterization of Origanum heracleoticum from Calabria, Southern Italy. Rap Com Mass Spect 1998;12(20): 1555-1563. 38. Nostro A, Roccaro AS, Bisignano G, Marino A, Cannatelli M, Pizzimenti FC et al. Effects of oregano, carvacrol and thymol on Staphylococcus aureus and Staphylococcus epidermidis biofilms. J Med Microbiol 2007; 56(4):519-523. doi: http://dx.doi.org/10.1099/jmm.0.46804-0 39. Matias FFE, Alves EF, Silva KNM

Open access

K. Englerová, R. Nemcová and D. Mudroňová

., Zmantar, T., Rouabhia, M., Mahdouani, K., Bakhrouf, A., 2007 : In vitro effect of pH and ethanol on biofilm formation by clinical ica-positive Staphylococcus epidermidis strains. Ann. Microbiol. , 57, 431—437. 7. Chen, Y. P., Hsiao, P. J., Hong, W. S., Dai, T. Y., Chen, M. J., 2012 : Lactobacillus kefiranofaciens M1 isolated from milk kefir grains ameliorates experimental colitis in vitro and in vivo . J. Dairy Sci. , 95, 63—74. 8. Cheow, W. S., Hadinoto, K., 2013 : Biofilm-like Lactobacillus rhamnosus probiotics encapsulated in alginate and