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Ewelina Wawryk-Gawda and Beata Wojcik

References 1. Ahn S.Y., Chang Y.S., Park W.S.: Mesenchymal stem cells transplantation for neuroprotection in preterm infants with severe intraventricular hemorrhage. Korean J Pediatr 57(6):251-6,2014. doi: 10.3345/kjp.2014.57.6.251. 2. Bajek A., Olkowska J., Drewa T.: Mesenchymal stem cells as a therapeutic tool in tissue and organ regeneration. Postepy Hig Med Dosw 24;65:124-32,2011. 3. Ball L.M., et al.: Multiple infusions of mesenchymal stromal cells induce sustained remission in children with steroid

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Amira Benahmed, Boualem Harfi, Ibtissem Benbelkacem, Abla Daas, Hocine Laouer and Abdelmalik Belkhiri


Mentha rotundifolia (L.) Huds is an aromatic plant used for its medicinal values. This study aims to select appropriate conditions for in vitro propagation of M. rotundifolia (L.) Huds and to evaluate yield and antioxidants activity of its essential oils (EOs).

The explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators 6-benzylaminopurine (BAP) and Gibberellin (GA3).

Hydrodistillated EOs obtained from acclimatized and mother plant, were evaluated for their antioxidant activity. Tests were performed on DPPH free radical-scavenging, ABTS and CUPRAC assays.

Shoot induction and multiplication were successfully carried out on MS medium supplemented with the following hormones combinations: 1 mg/l BAP, 1 mg/l GA3 and 0.5 mg/l BAP, 0.5 mg/l GA3, respectively. Stem length, nodes and leaves number measured from development vitroplant were 6.89 cm, 5.22 nodes and 11.92 leaves per vitroplant, respectively.

In vitro rooted plants were successfully acclimatized at a temperature of 23 ± 2°C and a long day photoperiod with a total survival rate exceeding 95%.

EO yield of acclimated plant varied between (0.88-1.49 ml/100 g dry matter) compared to wild plant (0.73 ml/100 g dry matter). The antioxidant potential of EOs from acclimated plant showed on DPPH free radical-scavenging, ABTS and CUPRAC assays values of (IC50: 4.18-24.93 mg/ml), (IC50: 0.51-1.56 mg/ml) and (A0.50: 0.34-2.71 mg/ml), respectively. In contrast, the wild plant exhibited on the same tests the values of (IC50: 10.35 mg/ml), (IC50: 0.12 mg/ml) and (A0.50: 0.99 mg/ml), respectively.

The results suggest that micropropagation of M. rotundifolia (L.) Huds can be an interesting alternative for producing important plant material with the possibility to modulate EO yield and its antioxidant potential for future commercial purposes.

Open access

Sabrie Zinabadinova, Viktoria Lavrinenko, Rostislav Kaminsky, Alina Korsak, Liudmyla Sokurenko and Yuri Chaikovsky

(1): 1-23. 10. Fernandes R., Costola-Souza C., Sarmento C., Gonçalves L., Favaron Ph. O., Miglino M. A. Placental tissues as sources of stem cells. Open Journal of Animal Sciences. 2012; 2: 166-173. 11. Guedes P., Oliveira B., Manso P., Caputo L., Cotta-Pereira G., Pelajo-Machado M. Histological analyses demonstrate the temporary contribution of yolk sac, liver, and bone marrow to hematopoiesis during chicken development. PLoS One. 2014; 9(3). 12. Lavrinenko V.E., Zinabadinova S.S., Shobat L.B., Sokurenko L.M., Chaikovsky Yu.B. Influence of

Open access

Kazuichi Yagi

brain-stem . In: G.H. Fromm, C.L. Faingold, R.A. Browning, W.M. Burnham (eds)., Epilepsy and the reticular formation . Alan R. Liss Inc., New York 1987, 25–38. Chevrie J.J., Aicardi J.: Childhood epileptic encephalopathy with slow spike-wave. A stastical study of 80 cases . Epilepsia, 1972, 13: 259–271. Crespel A., Gelisse P., Nikanorova M., Ferlazzo E., Genton P.: Lennox-Gastaut syndrome . In: M. Bureau, P. Genton, C. Dravet, A. Delgado-Escueta, C.A. Tassinari, P. Thomas, P. Wolf (eds), Epileptic syndromes in infancy, childhood and adolescence (5

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Renata Chalas, Ewelina Mielko, Joanna Zubrzycka-Wrobel and Jakub Nowak

.: Effect of Biodentine™ on the proliferation, migration and adhesion of human dental pulp stem cells. J. Dent., 42, 490, 2014.

Open access

Anna Malm, Anna Glowniak-Lipa, Izabela Korona-Glowniak and Tomasz Baj

of Algerian Thymus oils. Food Chem., 116, 714, 2009. 8. Lang G., Buchbauer G.: A review on recent research results (2008-2010) on essential oils as antimicrobial and antifungals. A review. Flavour Fragr. J., 27, 13, 2012. 9. Njume C. et al.: Volatile compounds in the stem bark of Sclerocarya birrea (Anacardiaceae) possess antimicrobial activity against drugresistant strains of Helicobacter pylori. Int. J. Antimicrob. Agents., 38, 319, 2011. 10. O`Donnell F. et al.: A study of the antimicrobial activity of selected

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Dobromir D. Dimitrov, Veselin I. Kirov, Borislav A. Ignatov, Boris D. Tsankov, Hyuliya E. Feradova, Vasya Vutova, Silviya P. Hristova, Vasil D. Nanev, Genoveva B. Valcheva and Tashko S. Deliyski

JC, Lee RJ, Prescott RJ, Doherty VR. A comparison of dermatologists', surgeons' and general practitioners' surgical management of cutaneous melanoma. Br J Dermatol. 2004; 151 (3):636-44. 11. Morton DL, Thompson JF, Essner R, Elashoff R, Stem S, Nieweg O, et al. Validation of the accuracy of intraoperative lymphatic mapping and sentinel lymphadenectomy for early-stage melanoma. Ann Surg. 1999;230(4):453-63. 12. Bostick P, Esner R, Glass E. Comparison of Blue Dye and Probe-Assisted Intraoperative Lymphatic Mapping in Melanoma to

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Mohsen Safaei, Mojtaba Taran, Mohammad Moslem Imani, Hedaiat Moradpoor, Amin Golshah and Prabhat Upadhyay

HR, Payandeh M, Ramezani M, Sadeghi M, Mahmoudiahmadabadi M, Sharifi R. Efficacy of palifermin on oral mucositis and acute GVHD after hematopoietic stem cell transplantation (HSCT) in hematology malignancy patients: a meta-analysis of trials. Wspolczesna Onkol. 2017;21(4):299-305. 7. Parsa N. Environmental factors inducing human cancers. Iran J Public Health. 2012;41(11):1-9. 8. Brawley OW. Avoidable cancer deaths globally. CA Cancer J Clin. 2011;61(2):67-8. 9. Gopalan MR, Karunakaran V, Prabhakaran A, Jayakumar KL. Prevalence of psychiatric

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Regina Komsa-Penkova, Svetla J. Todinova, Tonya D. Andreeva, Sashka B. Krumova, Stefka G. Taneva, Georgi M. Golemanov, Galia A. Georgieva, Nikolina M. Mihaylova, Andrey I. Tchorbanov and Pencho T. Tonchev

J, editors. Williams Hematology. 7th ed. New York: McGraw-Hill Professional; 2005. p. 1357-408. 4. Pompe T, Glorius S, Bischoff T, Uhlmann 1, Kaufmann M, Brenner S, et al. Dissecting the impact of matrix anchorage and elasticity in cell adhesion. Biophys J. 2009;97(8):2154-63. 5. Engler AJ, Sen S, Sweeney HL, Discher DE. Matrix elasticity directs stem cell lineage specification. Cell. 2006.126:677-89. 6. McBeath R, Pirone DM, Nelson CM, Bhadriraju K, Chen CS. Cell shape, cytoskeletal tension, and RhoA regulate stem

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Agnieszka Kolak, Michal Lozinski and Slawomir Mandziuk

Hematol Oncol Stem Cell Res. 2014;8 (1):46-50. 4. Lama D, Ong E. The role of surgical excision for the Krukenberg tumour: A case report. Int J Surg Case Rep. 2017;38:185-188. doi: 10.1016/j.ijscr.2017.07.046. 5. Matsushita H, Watanabe K, Wakatsuki A. Metastatic gastric cancer to the female genital tract (Review). Mol Clin Oncol. 2016;5 (5):495-499. doi: 10.3892/mco.2016.1035. 6. Kumar M, Kumar A, Maroules M, Abrina V, Kumar V. Postmenopausal vaginal bleeding as initial presentation of gastric cancer: a case report with literature review of prognostic