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K. Huňáková, M. Hluchý, M. Kuricová, K. Ševčík, J. Rosocha and V. Ledecký

REFERENCES 1. Admyre, C., Grunewald, J., Thyberg, J., Gripenbäck, S., Tornling G., Eklund A., 2003: Exosomes with major histo-compatibility complex class II and co-stimulatory molecules are present in human BAL fluid. Eur. Respir. J. , 22, 578—583. 2. Bruno, S., Grange, C., Deregibus, M. C., 2009: Mesenchymal stem cell-derived microvesicles protect against acute tubular injury. J. Am. Soc. Nephrol. , 20, 1053—1067. 3. Caby, M. P., Lankar, D., Vincendeau-Scherrer, C., Raposo, G., Bonnerot, C., 2005: Exosomal-like vesicles are present in

Open access

M. Giretová, Ľ. Medvecký, E. Petrovová, D. Čížková, D. Mudroňová and J. Danko

REFERENCES 1. Barry, F. P., Murphy, J. M., 2004: Review Mesenchymal stem cells: clinical applications and biological characterization. Int. J. Biochem. Cell Biol , 36, 568—584. DOI: 10.1016/j.biocel.2003.11.001. 2. Bornes, T. D., Jomha, N. M., Mulet-Sierra, A., Adesida, A. B., 2016: Optimal seeding densities for in vitro chondrogenesis of two- and three-dimensional-isolated and expanded bone marrow-derived mesenchymal stromal stem cells within a porous collagen scaffold. Tissue Engn. C: Methods , 22, 208— 220. DOI: 10.1089/ten.tec.2015

Open access

Anatoliy Mazurkevych, Mykola Malyuk, Natalia Bezdieniezhnykh, Lyubov Starodub, Yuriy Kharkevych, Evgen Brusko, Magdalena Gryzińska and Andrzej Jakubczak

References 1. Andraszek K., Gryzinska M., Danielewicz A., Batkowska J., Smalec E.: Age-dependent stability of nucleoli and global DNA methylation level in spermatocytes of the domestic horse (Equus caballus). Can J Anim Sci 2016, 96, 215–220. 2. Barberini D.J., Freitas N.P., Magnoni M.S., Maia L., Listoni A.J., Heckler M.C., Sudano M.J., Golim M.A., da Cruz Landim-Alvarenga F., Amorim RM.: Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential. Stem Cell Res

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Nasim Malekmohamadi, Alireza Abdanipour, Mehrdad Ghorbanlou, Saeed Shokri, Reza Shirazi, Eva Dimitriadis and Reza Nejatbakhsh

References de Rooij DG. Proliferation and differentiation of spermatogonial stem cells. Reproduction 121, 347–354, 2001. de Sousa Lopes SM, Roelen BA, Monteiro RM, Emmens R, Lin HY, Li E, Lawson KA, Mummery CL. BMP signaling mediated by ALK2 in the visceral endoderm is necessary for the generation of primordial germ cells in the mouse embryo. Genes Dev 18, 1838–1849, 2004. Dudley BM, Runyan C, Takeuchi Y, Schaible K, Molyneaux K. BMP signaling regulates PGC numbers and motility in organ culture. Mech Dev 124, 68–77, 2007. Fabbri A, Knox G

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D. O. Olayemi, M. M. Onakpa and O. C. Jegede

Abstract

The development of host resistance to anthelmintics and the increasing cost of commercial anthelmintics have encouraged the need for the in vitro anthelmintic evaluation of crude extract and fractions of Hymenodictyon pachyanta plant as alternative drugs against Haemonchus contortus. H. contortus is one of the most prevalent and highly pathogenic parasitic nematodes in small ruminant farming globally. H. pachyanta stem bark is a prospective plant used by the local and indigenous farmers of Nsukka, Enugu state, Nigeria. The stem bark of H. pachyanta were collected, dried, pulverized and extracted with 80 % methanol. The purpose of this study was to investigate the in vitro anthelmintic effects of these crude extract and fractions against H. contortus in sheep and goats. The two extracts (crude and fractions) of H. pachyanta were tested by the egg hatch assay (EHA) and the larval development inhibition assays (LDIA) and to compared the results with albendazole (as the positive control). The concentrations for the crude extract and albendazole used for this study were 0.78, 1.56, 3.125, 6.25 and 12.5 mg.ml−1. The results demonstrated that the crude extracts, fractions and albendazole all at the concentration doses of 12.5 mg.ml−1 produced 100 % inhibition of egg hatching and larval development. Statistically, there was no significant difference (P > 0.05) in the mean percentage inhibition of egg hatching and larval development inhibition of the crude extracts and fractions when compared with albendazole. However, a significant difference (P < 0.05) was observed with n-butanol fraction which inhibited 96.17 % of egg hatchability. All of the extracts and albendazole showed ovicidal and larvicidal effects and were able to induce over 50 % of the egg hatching and mortality of larvae at the concentration ranges of 0.78—12.5 mg.ml−1. The results obtained from our study suggest that H. pachyanta had ovicidal and larvicidal activity against H. contortus and that the bioactive plants compounds responsible for this effect could be attributed to the presence of tannins, alkaloids and the saponins contained in the crude extracts.

Open access

I. Šulla, V. Balik and M. Šarišský

REFERENCES 1. Animal Protection Act of Slovakia No. 15/1995, part 39 (In Slovak), 1250—1255. 2. Caplan, A. I., Dennis, J. E., 2006: Mesenchymal stem cells as trophic mediators. J. Cell. Biochem. , 98, 1076—1084. 3. Chesier, S. H., Kalani, M. Y. S., Lim, M., Ailles, L., Huhn, S. L., Weissman, I. L., 2009: A neurosurgeon’s guide to stem cells, cancer stem cells, and brain tumor stem cells. Neurosurgery , 65, 237—250. 4. Čížková, D., Rosocha, J., Vanický, I., Jergová, S., Čížek, M., 2006: Transplants of human mesenchymal stem cells

Open access

Ning Ma, Xin Li, Hong-bin Wang, Li Gao and Jian-hua Xiao

of iletamine-zolazepam-xylazine-tramadol combination on biochemical and haematological parameters in cats. Bull Vet Inst Pulawy 2012, 56, 369–372. 17. Livak K.J., Schmittgen T.D.: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods-A Companion To Methods in Enzymology 2001, 25, 402-408. 18. Madhusoodanan K.S., Murad F.: NO-cGMP signaling and regenerative medicine involving stem cells. Neurochem Res 2007, 32, 681–694. 19. Nakayama T., Hashimoto T., Nagai Y.: Involvement of

Open access

I. Šulla, V. Balik, S. Horňák and V. Ledecký

REFERENCES 1. Adams, M. M., Hicks, A. L., 2005: Spasticity after spinal cord injury. Spinal Cord , 43, 577—586. 2. Akhtar, A. Z., Pippin, J. J., Sandusky, C. B., 2008: Animal models in spinal cord injury: a review. Rev. Neurosci. , 19, 47—60. 3. Badner, A., Vawda, R., Laliberte, A., Hong, J., Mikhail, M., Jose, A., et al., 2016: Early intravenous delivery of human brain stromal cells modulates systemic inflammation and leads to vasoprotection in traumatic spinal cord injury. Stem Cells Transl. Med. , 5, 991—1003. 4. Bockurt, G

Open access

S. Flešárová and D. Maženský

Abstract

The aim of this study was to describe the anatomical arrangement of the branches arising from the dorsal surface of the aorta abdominalis in the rabbit and the hare. The study was carried out on ten adult rabbits and ten adult European hares using the corrosion technique. After the euthanasia, the vascular network was perfused with saline. After polymerization of the casting medium, the maceration was carried out in a KOH solution. We found different variations in; the number of arteries, level of their origin and arrangement. The aa. lumbales of the same level arose by means of a common trunk or their origin was independent. The aa. lumbales VI or aa. lumbales VI et VII originated also from the a. sacralis mediana. By aa. lumbales we found an important interspecies difference in; number, diameter, ramification and density of dorsal branches, which are designated for the dorsal muscles of the body stem. All listed parameters of branches were higher in the hare. This anatomical arrangement of dorsal branches is adapted to the higher movement activity of the hare. According to our results, it can be concluded that the anatomical arrangement of the branches of the aorta abdominalis shows a higher number of variations in the domesticated rabbit in comparison with the hare.

Open access

Anna Snarska, Dominika Wysocka and Liliana Rytel

.M., Sadik N.A., Azzam M..: The therapeutic effects of bone marrow-derived mesenchymal stem cells and simvastatin in a rat model of liver fibrosis. Cell Biochem Biophys 2014, 68, 111–25. 12. Mundy G., Garrett R., Harris S., Chan J., Chen D., Rossini G., Boyce B., Zhao M., Gutierrez G.: Stimulation of bone formation in vitro and in rodents by statins. Science 1999, 286, 1946–1949. 13. Newman A., Clutterbuck R.D., Powles R.L., Chan J., Chen D., Rossini G., Boyce B., Zhao M., Gutierrez G.: Selective inhibition of primary acute myeloid leukaemia cell growth by