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K. Huňáková, M. Hluchý, M. Kuricová, K. Ševčík, J. Rosocha and V. Ledecký

REFERENCES 1. Admyre, C., Grunewald, J., Thyberg, J., Gripenbäck, S., Tornling G., Eklund A., 2003: Exosomes with major histo-compatibility complex class II and co-stimulatory molecules are present in human BAL fluid. Eur. Respir. J. , 22, 578—583. 2. Bruno, S., Grange, C., Deregibus, M. C., 2009: Mesenchymal stem cell-derived microvesicles protect against acute tubular injury. J. Am. Soc. Nephrol. , 20, 1053—1067. 3. Caby, M. P., Lankar, D., Vincendeau-Scherrer, C., Raposo, G., Bonnerot, C., 2005: Exosomal-like vesicles are present in

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Marius Mihai Harpa, Ionela Movileanu, Leslie Neil Sierad, Ovidiu Simion Cotoi, Horatiu Suciu, Carmen Sircuta, Terezia Preda, Dan Nistor, Klara Branzaniuc, Radu Deac, Michael Dandel, Simona Gurzu, Lucian Harceaga, Peter Olah, Agneta Simionescu and Dan Simionescu

valves with tissue-engineered grafts. Tissue Eng Part A. 2013 Aug;19(15-16):1686-94. DOI: 10.1089/ten.tea.2012.0074 7. Tedder ME, Simionescu A, Chen J, Liao J, Simionescu DT. Assembly and testing of stem cell-seeded layered collagen constructs for heart valve tissue engineering. Tissue Eng Part A. 2011 Jan;17(1-2):25-36. DOI: 10.1089/ten.tea.2010.0138 8. Ku CH, Johnson PH, Batten P, Sarathchandra P, Chambers RC, Taylor PM, et al. Collagen synthesis by mesenchymal stem cells and aortic valve interstitial cells in response to mechanical stretch. Cardiovasc

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M. Giretová, Ľ. Medvecký, E. Petrovová, D. Čížková, D. Mudroňová and J. Danko

REFERENCES 1. Barry, F. P., Murphy, J. M., 2004: Review Mesenchymal stem cells: clinical applications and biological characterization. Int. J. Biochem. Cell Biol , 36, 568—584. DOI: 10.1016/j.biocel.2003.11.001. 2. Bornes, T. D., Jomha, N. M., Mulet-Sierra, A., Adesida, A. B., 2016: Optimal seeding densities for in vitro chondrogenesis of two- and three-dimensional-isolated and expanded bone marrow-derived mesenchymal stromal stem cells within a porous collagen scaffold. Tissue Engn. C: Methods , 22, 208— 220. DOI: 10.1089/ten.tec.2015

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Ondine Lucaciu, Dan Gheban, Olga Soriţau, Mihaela Băciuţ, Radu Septimiu Câmpian and Grigore Băciuţ

-9612(00)00102-2 4. Shastri P. Future of Regenerative Medicine: Challenges and Hurdles. Artificial Organs. 2006;30(10):828-34. DOI: 10.1111/j.1525-1594.2006.00307.x 5. Polini A, Pisignano D, Parodi M, Quarto R, Scaglione S. Osteoinduction of human mesenchymal stem cells by bioactive composite scaffolds without supplemental osteogenic growth factors. PLoS One. 2011;6(10):e26211. doi:10.1371/journal.pone.0026211 DOI: 10.1371/journal.pone.0026211 6. Firoozabadi R, Morsher S, Engelke K, Prevrhal S, Fierlinger A, Miclau T 3rd, Genant HK. Qualitative and

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Anatoliy Mazurkevych, Mykola Malyuk, Natalia Bezdieniezhnykh, Lyubov Starodub, Yuriy Kharkevych, Evgen Brusko, Magdalena Gryzińska and Andrzej Jakubczak

References 1. Andraszek K., Gryzinska M., Danielewicz A., Batkowska J., Smalec E.: Age-dependent stability of nucleoli and global DNA methylation level in spermatocytes of the domestic horse (Equus caballus). Can J Anim Sci 2016, 96, 215–220. 2. Barberini D.J., Freitas N.P., Magnoni M.S., Maia L., Listoni A.J., Heckler M.C., Sudano M.J., Golim M.A., da Cruz Landim-Alvarenga F., Amorim RM.: Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential. Stem Cell Res

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Mihaela Cîrstea, Adriana Coliță, Bogdan Ionescu, Didona Vasilache, Camelia Dobrea, Cerasela Jardan and Mihaela Dragomir

Cytogenetic Nomenclature. Basel, Switzerland: 2013: 78-79. 20. Kekre N, Ho VT. Allogeneic hematopoietic stem cell transplantation for myelofibrosis and chronic myelomonocytic leukemia. Am J Hematol. 2015 Nov;91(1):123-30. DOI: 10.1002/ajh.24215. 21. Symeonidis A, van Biezen A, de Wreede L, Piciocchi A, Finke J, Beelen D, et al. Achievement of complete remission predicts outcome of allogeneic haematopoietic stem cell transplantation in patients with chronic myelomonocytic leukaemia. A study of the chronic malignancies working party of the

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Peter J. Späth

.jaci.2013.08.032 42. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25;126(4):663-76. DOI: 10.1016/j.cell.2006.07.024 43. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007 Nov 30;131(5):861-72. DOI: 10.1016/j. cell.2007.11.019 44. Pessach IM, Ordovas-Montanes J, Zhang SY, Casanova JL, Giliani S, Gennery AR, et

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D. O. Olayemi, M. M. Onakpa and O. C. Jegede

Abstract

The development of host resistance to anthelmintics and the increasing cost of commercial anthelmintics have encouraged the need for the in vitro anthelmintic evaluation of crude extract and fractions of Hymenodictyon pachyanta plant as alternative drugs against Haemonchus contortus. H. contortus is one of the most prevalent and highly pathogenic parasitic nematodes in small ruminant farming globally. H. pachyanta stem bark is a prospective plant used by the local and indigenous farmers of Nsukka, Enugu state, Nigeria. The stem bark of H. pachyanta were collected, dried, pulverized and extracted with 80 % methanol. The purpose of this study was to investigate the in vitro anthelmintic effects of these crude extract and fractions against H. contortus in sheep and goats. The two extracts (crude and fractions) of H. pachyanta were tested by the egg hatch assay (EHA) and the larval development inhibition assays (LDIA) and to compared the results with albendazole (as the positive control). The concentrations for the crude extract and albendazole used for this study were 0.78, 1.56, 3.125, 6.25 and 12.5 mg.ml−1. The results demonstrated that the crude extracts, fractions and albendazole all at the concentration doses of 12.5 mg.ml−1 produced 100 % inhibition of egg hatching and larval development. Statistically, there was no significant difference (P > 0.05) in the mean percentage inhibition of egg hatching and larval development inhibition of the crude extracts and fractions when compared with albendazole. However, a significant difference (P < 0.05) was observed with n-butanol fraction which inhibited 96.17 % of egg hatchability. All of the extracts and albendazole showed ovicidal and larvicidal effects and were able to induce over 50 % of the egg hatching and mortality of larvae at the concentration ranges of 0.78—12.5 mg.ml−1. The results obtained from our study suggest that H. pachyanta had ovicidal and larvicidal activity against H. contortus and that the bioactive plants compounds responsible for this effect could be attributed to the presence of tannins, alkaloids and the saponins contained in the crude extracts.

Open access

Marius Harpa, Ionela Movileanu, Leslie Sierad, Ovidiu Cotoi, Horațiu Suciu, Terezia Preda, Dan Nistor, Carmen Sircuța, Klara Brânzaniuc, Radu Deac, Simona Gurzu, Lucian Harceaga, Peter Olah, Dan Simionescu, Michael Dandel and Agneta Simionescu

Valve Scaffolds Obtained by Complete Decellularization of Porcine Aortic Roots in a Novel Differential Pressure Gradient Perfusion System. Tissue Eng Part C Methods. 2015 Dec;21(12):1284-96. DOI: 10.1089/ten.tec.2015.0170. 6. Gimble J, Guilak F. Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy. 2003;5(5):362-9. DOI: 10.1080/14653240310003026. 7. Schoen FJ. Heart valve tissue engineering: quo vadis? Curr Opin Biotechnol. 2011 Oct;22(5):698-705. DOI: 10.1016/j.copbio.2011

Open access

Mihaela Cîrstea, Adriana Coliță, Bogdan Ionescu, Alexandra Ghiaur, Didona Vasilescu, Camelia Dobrea, Cerasela Jardan, Mihaela Dragomir, Anca Gheorghe, Zsofia Várady and Anca Roxana Lupu

Abstract

In the 2016 revision of the World Health Organization classification the term therapy-related myeloid neoplasia (t-MN) defines a subgroup of acute myeloid leukemia (AML) comprising patients who develop myelodysplastic syndrome (MDS-t) or acute myeloid leukemia (AML-t) after treatment with cytotoxic and/or radiation therapy for various malignancies or autoimmune disorders. We report the case of a 36 year old patient with t-MN (t-MDS) after achieving complete remission (CR) of a PML-RARA positive acute promyelocytic leukemia (APL) at 32 months after diagnosis. Initially classified as low risk APL and treated according to the AIDA protocol - induction and 3 consolidation cycles - the patient achieved a complete molecular response in September 2013 and started maintenance therapy. On follow-up PML-RARA transcript remained negative. In January 2016 leukopenia and thrombocytopenia developed and a peripheral blood smear revealed hypogranular and agranular neutrophils. Immunophenotyping in the bone marrow aspirate identified undifferentiated blast cells that did not express cytoplasmic myeloperoxidase. The cytogenetic study showed normal karyotype. The molecular biology tests not identified PMLRARA transcript. A diagnosis of t-MDS (AREB-2 - WHO 2008) was established. Treatment of AML was started with 2 “3+7” regimens and 1 MEC cycle. Two months from diagnosis, while in CR, an allogeneic HSCT from an unrelated HLA compatible donor was performed after myeloablative regimen. An unfavorable clinical evolution was followed by death on day 9 after transplantation. The occurrence of t-MNs during CR of APL represents a particular problem in terms of follow-up and differential diagnosis of relapse and constitutes a dramatic complication for a disease with a favorable prognosis.

This work was supported by the grants PN 41-087 /PN2-099 from the Romanian Ministry of Research and Technology