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Human term placenta as source of stem cells for regenerative medicine

References 1. Parolini O, De D, Rodrigues MF, and Caruso M Placental Stem/Progenitor Cells: Isolation and Characterization, in Perinatal Stem Cells, A. Atala and S.V. Murphy, Editors. 2014, Springer Science: New York. p.373. 2. Debashree D, Kmiecik G, Cargnoni A, and Parolini O Placenta-Derived Cells and Their Therapeutic Applications, in Gene and Cell Therapy: Therapeutic Mechanisms and Strategies, N.S. Templeton, Editor. 2015, CRC Press. 3. Parolini O and Soncini M Placenta as a Source of Stem Cells and as a

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Stem cells migration to the brain through cranial nerves endings

References 1. Dennie D, Louboutin JP, Strayer DS Migration of bone marrow progenitor cells in the adult brain of rats and rabbits. // World J. Stem Cells. 8(4):136-5, 2016. 2. Chung TN, Kim JH, Choi BY, Chung SP, Kwon SW, Suh SW. Adipose- derived mesenchymal stem cells reduce neuronal death after transient global cerebral ischemia through prevention of blood-brain barrier disruption and endothelial damage. // Stem Cells Transl Med. 4(2):178-85, 2015. 3. Wei ZZ, Gu X, Ferdinand A, Lee JH, Ji X, Ji XM, Yu SP, Wei

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Soils from HNV agriculture systems as source of microorganisms with antifungal activity

agricultural soils. T&A Constable Ltd. Edinburg, London 1970. 5. Watanabe T. Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species 2nd ed. 2002; CRC PRESS. 6. Matei S. Determination of soil respiration and microbial biomass. In: Dumitru M, Manea A(coord). Methods of chemical and microbiological analysis (utilized in soil monitoring system), (in Romanian). Ed. SITECH, Craiova 2011; p. 283-288. 7. Phuoc NT. Biological control of tomato root and stem rot caused by Sclerotinium rolfsii Sacc., ARC

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Switching from petro-plastics to microbial polyhydroxyalkanoates (PHA): the biotechnological escape route of choice out of the plastic predicament?

, Wang HMD, Lan JCW. Investigation and characterization of plasma-treated poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate- co -3-hydroxyvalerate) Biopolymers for an in vitro cellular study of mouse adipose-derived stem cells. Polymers 2018; 10(4): 355. 130. Bhatia SK, Wadhwa P, Hong JW, Hong YG, Jeon JM, Lee ES, Yang YH. Lipase mediated functionalization of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) with ascorbic acid into an antioxidant active biomaterial. Int J Biol Macromol 2019; 123(15): 117-123. 131. Zhang J, Cao Q, Li S, Lu X, Zhao Y, Guan JS

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Cell wall gene expression in two sub-species of flax

Abstract

In this work, we attempted to elucidate mechanisms of flax fiber biogenesis by measuring, using qPCR, expression of the cellwall related genes (cellulose synthase, cellulose synthase-like and chitinase-like genes) in stems of two contrasting fiber quality subspecies of flax (Linum usitatissimum L.). We studied elongatum Vav. et Ell. (cultivar Blakit, Belarus) and crepitans Boenn. (dehiscent flax) subspecies, which are differed in the height of plants, width of stems etc. Amongst all measured genes chitinase- like Ctl19 and Ctl21 genes showed drastic difference in expression between stems of the two flax varieties, showing higher expression level in the fiber flax versus the dehiscent flax. In contrast, cellulose synthase-like CslG4 gene had lower expression levels in the stem of fiber flax than in dehiscent flax. We suggest that hemicellulose composition and cellulose - non-cellulose glycan organisation can vary between stalk cells of different flax subspecies.

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Analysis of public RNA-seq data in studies of flax fiber biogenesis

Abstract

In this work we used publicly available raw RNA-seq data to elucidate mechanisms of flax fiber biogenesis by measuring expression of cell-wall related genes (cellulose synthase, cellulose synthase-like and chitinase-like genes) in stem of flax (Linum usitatissimum cv. Bethune). Using public RNA-sequence data we have quantified and characterised the expression of the specific cell-wall genes in the top, middle and bottom parts of the Bethune flax stem. The most prominent findings are: Secondary cell-wall cellulose synthase (CesA) genes are expressed differentially in phloem and xylem in all parts of Bethune stem, in contrast with primary cell-wall cellulose synthase genes. Total expression level of cellulose synthase-like (Csl) genes is tissue invariant (although, CslG and CslE are differentially expressed) and smaller than the total expression of cellulose synthase genes. The CslD2D3 subgroup dominates total expression of CslD genes in both xylem and phloem. Expression levels of all expressed chitinase-like (Ctl) genes are tissue dependent in all parts of stem. Total expression level of chitinase-like genes is much higher than expression of cellulose synthase and cellulose synthase-like genes in both tissues.

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Genetics of amyotrophic lateral sclerosis: more than twenty years of studies

Abstract

Amyotrophic lateral sclerosis, also known as motor neuron disease or Lou Gehrig’s disease, is an adult-onset neurodegenerative disease that targets motor neurons in the spinal cord, cortex and brain stem. Selective degeneration of corticospinal (upper) and spinal (lower) motor neurons manifests as a linear decline in muscular function, eventually resulting in paralysis, speech and swallowing deficits and death, usually from impaired respiratory function, over a time course of approximately 3-5 years.

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Health risks of leafy vegetable Alternanthera philoxeroides (Alligator weed) rich in phytochemicals and minerals

Abstract

Alternanthera philoxeroides (alligator weed) grows abundantly in polluted Peri-urban Greater Hyderabad, India. It is collected at no cost and sold in the market as a leafy vegetable in the name of “Ceylon Spinach”. The plant accumulates iron (Fe), manganese (Mn), cadmium (Cd), lead (Pb) and zinc (Zn) in roots and leaves. Soil samples were analyzed for pH, EC, available nitrogen (N), phosphorus (P) and potassium (K) and showed significant metal concentrations of Pb, Mn and Zn, which varied from one location to another. The metal accumulation order in the plants is root>leaf>stem in all the studied sites. The results revealed that the massive roots of alligator weed are effective in the bioconcentrating Fe, Mn, Zn, Pb and Cd, although the plant parts are rich in nutraceuticals like phenolics and antioxidants. Therefore, low income community prefers to consume it as vegetable. However, its consumption as a leafy vegetable can cause health risks.

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Dosimetric comparison of three dimensional conformal radiotherapy and intensity modulated radiotherapy in high grade gliomas

Dosimetric comparison of three dimensional conformal radiotherapy and intensity modulated radiotherapy in high grade gliomas

Treatment of high-grade gliomas continues to be frustrating for the clinician as the medial survival stands at a dismal 14.5 months for glioblastoma multiforme (GBM) with the current standard of care. Given the high dose and generous margins required to be irradiated, three dimensional conformal radiotherapy (3DCRT) has become standard practice. Radiation dose escalation beyond 60 Gy, by means of stereotactic or intensity modulated radiotherapy (IMRT) boost, has not yielded clinically significant benefits in terms of local control or survival. At the same time, the potential of IMRT to spare normal tissues such as the brain stem and the optic apparatus makes it an attractive tool for modern radiation oncologists in seeking to improve post-radiotherapy quality of life. At our centre, we have been treating a large number of cases of high grade glioma with 3DCRT and IMRT for the last several years. The present study has been an effort to understand any potential benefits that IMRT, even without dose escalation, can offer.

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