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The number of PON1 mutant alleles, but not PON1 phenotype, is associated with Gensini score of coronary damage

Abstract

Objectives. The aim of this study was to examine the effects of single nucleotide polymorphisms (SNPs) of PON1 gene at the level of promoter region (‒909 and ‒832) and of first exon (+575, A20352G, resulting Q192R substitution) on paraoxonase-1 (PON1) activities in 53 patients with angiographycally proven coronary heart disease (CHD) and 17 free-CHD subjects. Methods and Results. Serum PON1 arylesterase (Ar-ase) and salt-stimulated paraoxonase (ssPO-ase) activities were assessed with manual spectrophotometric methods, by using phenyl acetate and paraoxon as substrates. Common serum biochemical markers were assayed by enzymatic methods using commercial kits, on a Roche/Hitachi 912 Auto Analyzer. PON1 genotypes were determined by PCR and nucleotide sequencing of the amplicons with an ABI PRISMTM 310 Genetic Analyzer and a BigDye® Terminator v3.1 Cycle Sequencing Kit. The severity of coronary artery stenosis was assessed and classified using the Gensini score. We found no significant differences in the PON1 activities and -909(G→C), -832(G→A) and +575(A→G) PON1 polymorphisms between CHD and CHD-free groups. Considering all investigated subjects, we found that -909(G→C) and +575(A→G) SNPs had statistically significant effects on Ar-ase activity and PO-ase activity, respectively. In a multiple regression model we found that diabetes, LDL-cholesterol and the number of mutant alleles were significant independent determinants of the Gensini score. A significant positive correlation was observed only between the Gensini score and the number of mutant alleles. Conclusions. There are no differences between CHD and CHD-free groups regarding PON1 genotypes and phenotypes but the increasing number of PON1 mutant alleles is an important factor in determining the severity of coronary damage.

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FGB -455 G>A and GP IIIa PIA1/A2 polymorphisms in a group of Romanian stroke patients

Abbreviations EU- European Union GP- glycoprotein IHD- ischemic heart disease FGG- fibrinogen gamma FGA- fibrinogen alpha FGB- fibrinogen beta CVD- cerebrovascular disease vWF- von Willenbrand Factor PCR-RFLP- polymerase chain reaction-restriction fragment length polymorphism χ 2 - Chi-square test OR- odds ratio CI- confidence interval SNP- single nucleotide polymorphism References 1. Ohman EM, Bhatt DL, Steg PG, Goto S, Hirsch AT, Liau CS, et al. The Reduction of Atherothrombosis for Continued Health (REACH) Registry: an

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Polymorphisms of AZIN1 rs2679757 and TRPM5 rs886277 are Associated with Cirrhosis Risk in Chinese Patients with Chronic Hepatitis B

References 1 Poynard T, Bedossa P, Opolon P. Natural history of liver fibrosis progression in patients with chronic hepatitis C. The OBSVIRC, METAVIR, CLINIVIR, and DOSVIRC groups. Lancet 1997;349:825-832. 2 Powell EE, Edwards-Smith CJ, Hay JL, Clouston AD, Crawford DH, Shorthouse C, et al. Host genetic factors influence disease progression in chronic hepatitis C. Hepatology 2000;31:828-833. 3 Day CP. Genetic studies to identify hepatic fibrosis genes and SNPs i n human populations. Methods Mol Med 2005

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Melatonin Receptor 1B Gene Polymorphisms, Haplotypes and Susceptibility to Schizophrenia

, Rifai N, Regan MM, Price NJ, Dinges DF, et al. Effect of sleep loss on C-reactive protein, an inflammatory marker of cardiovascular risk. Journal of the American College of Cardiology. 2004;43(4):678-83. DOI: 10.1016/j. jacc.2003.07.050 16. Spiegel K, Knutson K, Leproult R, Tasali E, Van Cauter E. Sleep loss: a novel risk factor for insulin resistance and Type 2 diabetes. Journal of applied physiology. 2005;99(5):2008-19. DOI: 10.1152/japplphysiol.00660.2005 17. Liu C, Wu Y, Li H, Qi Q, Langenberg C, Loos RJ, et al. MTNR1B rs10830963 is

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The mysterious relation between inflammation and prostate cancer

neoplastic cells [ 28 ]. Other studies have reported that high expression of chemokine receptors may be necessary to activate cancer progressing signals. For instance, advanced aggressive PC cells express higher levels of CCR2 compared to less-aggressive gland cells, and they also show increase in metastatic capacity compared to localized PC [ 33 ]. Furthermore, increased CXCL8 and CCL2 levels in serum have been shown to be potential prognostic markers of a highly progressive PC [ 34 , 35 ]. Therefore, the role of chemokines in PC is very significant. 3 Cytokines

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Roles of African swine fever virus structural proteins in viral infection

.V., Sánchez-Vizcaino J.M., Ståhl K.: Development of a suspension microarray for the genotyping of African swine fever virus targeting the SNPs in the c-terminal end of the p72 gene region of the genome. Transbound Emerg Dis 2013, 60, 378–383. 46. Leitão A., Cartaxeiro C., Coelho R., Cruz B., Parkhouse R.M., Portugal F., Vigário J.D., Martins C.L.: The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response. J Gen Virol 2001, 82, 513–523. 47. Lithgow P., Takamatsu H., Werling D

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