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Copro-prevalence and risk factor assessment of gastrointestinal parasitism in Indian domestic pigs

/absent) and cleanliness status of the farm (present/absent) based on the scores like regular removal of waste material from sheds and drainage etc. was collected through predesigned questionnaire. Sample processing and examination The faecal samples were collected immediately after defecation, and subjected to qualitative faecal examination techniques (concentration-flotation and sedimentation technique) as per standard protocols ( Soulsby, 1982 ). Quantification of strongyle infection was done by McMaster technique ( MAFF,1986 ). Faecal cultures were prepared by

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Biomolecular changes and cortical neurodegenerative lesions in Trichinella spiralis infected BALB/c mice: a preliminary study elucidating a potential relationship between systemic helminthic infections and idiopathic Parkinson’s


Idiopathic Parkinson’s (IP) is a neurodegenerative disease that is suspected to be due to exposure to infections during early life. Toxoplasmosishas been the only suspected parasitic infection in IP (Celik et al., 2010). Recently, some non-central nervous system bacterial and viral infections have been incriminated in IP (Çamcı & Oğuz, 2016). So in the current study, we tried to explore if the systemic infl ammatory reactions triggered by some helminths like Trichinella spiralis can induce Parkinsonian lesions in the brain, especially that the cerebral complications have been reported in 10-20% of Trichinella spiralis infected patients . An experimental study was designed to assess the neurodegenerative and biomolecular changes that may occur in Trichinella spiralis infected BALB/C mice in comparison to rotenone induced PD model and apparently healthy ones. The motor affection was significantly lesser in the Trichinella infected mice than the Parkinson’s model, but when the catalepsy score was calculated (through the grid and bar tests) it was found to be significantly higher in the infected mice than in the healthy ones. A significant increase in the blood advanced oxidative protein products (AOPP), IFN-γ, TGF-β, and brain DNA fragmentation was also detected in the Trichinella spiralis infected mice. After histopathological examination, a significant increase in the cortical apoptotic neurons and Lewy’s body were observed in the Trichinella infected and the rotenone induced Parkinson’s model sections. A significant decrease in the immunohistochemical expression of the tyrosine hydroxylase expression in the brain sections and the ELISA measured dopamine level in the brain homogenate was also reported in the infected mice group. This study findings may collectively suggest that the systemic inflammatory reactions and the oxidative stresses associated with some systemic helminthic infections like trichinellosis are possible to precipitate neurodegenerative lesions and biomolecular changes in the brain , and manifest with IPD later in life.

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The fine structures of Cystocaulus ocreatus (Nematoda: Protostrongylidae) and the related lung pathology

(Protostronglylidae, Nematoda) using macroscopic lesion scores. Vet. Res., 28: 143–148 [4] Guttekova, A., Zmoray, I. (1974): Comparative study of the ultrastructure of muscle cells in pneumohelminths. Helminthologia, 15: 585–602 [5] Hernandez de Lujan, S., Muela De la, N., Ferre, I. (2001): Helminth parasites in goats in Valencia region. Med. Vet., 18: 476–482 [6] Levine, N. D. (1968): Nematode Parasites of Domestic Animals and of Man. Burgess Publishing Company, USA. [7

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Syphacia muris infection delays the onset of hyperglycemia in WBN/Kob-Lepr fa rats, a new type 2 diabetes mellitus model

. (2014): The inflammatory status score including IL-6, TNF-a, osteopontin, fractalkine, MCP-1 and adiponectin underlies whole-body insulin resistance and hyperglycemia in type 2 diabetes mellitus. Acta Diabetol , 51: 123 - 131. DOI: 10.1007/s00592-013-0543-1 Dl Marzo, V. (2008): The endocannabinoid system in obesity and type 2 diabetes. Diabetologia , 51: 1356 - 1367. DOI 10.1007/s00125-008-1048-2 Donath, M. Y., Shoelson, S. E. (2011) Type 2 diabetes as an inflammatory disease. Nat. Rev. Immunol. , 11: 98 - 107. DOI: 10.1038/nri2925 International Diabetes

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Seasonal variability of the composition and structure of parasite communities of red porgy, Pagrus pagrus (Perciformes: Sparidae) off Brazil

Demersais com Espinhel- de-fundo na Zona Econômica Exclusiva da Região Sudeste- Sul do Brasil. Série Documentos Revizee - Score Sul, Instituto Oceanográfi co - USP, São Paulo: 93 - 110. BAKUN, A., PARRISH, R.H. (1990): Comparative studies of coastal pelagic fi sh reproductive habitats: the Brazilian sardine (Sardinella aurita). J. Cons. int. Explor. Mer., 46: 269 - 283. DOI: 10.1093/ icesjms/46.3.269 BARBER, I., POULIN, R. (2002): Interactions between fi sh, parasites and disease. In: HART, P.J.B., REYNOLDS, J.D. (Eds) Handbook of Fish

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Crenosoma vulpis infection in a four-month old puppy

with the manufacturer’s instructions, and partial mitochondrial 12S rRNA (~330 bp) and nuclear 18S rRNA (~1700 bp) genes were amplified as previously described ( Latrofa et al ., 2015 ). Results On physical examination the dog was bright, alert and responsive with a body condition score of 3/9. Her inspiratory effort was increased and an unproductive cough with terminal retch was noticed during the consultation, although there was no response on tracheal pinch. On thoracic auscultation, normal respiratory sounds were slightly exaggerated over the entire lung

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Meloidogyne luci, a new infecting nematode species on common bean fields at Paraná State, Brazil

min) ( Cherry et al., 1997) . DNA sequences were analyzed using BLASTn megablast ( http:// ) and deposited in GenBank (KP165404 and KP165405). The ITS1 sequences were aligned using CLUSTAL W (Tompson et al., 1997). Dendrogram was obtained by MEGA6.06 ( Tamura etal 2013) . Model with lowest BIC (Bayesian Information Criterion) scores was considered to describe the substitution pattern. Then, a phylogenetic tree was constructed using Maximum Likelihood with Kimura 2-parameter model (Gamma distribution) and complete deletion

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Helminth fauna of Chelonia mydas (Linnaeus, 1758) in the south of Espírito Santo State in Brasil

, level of 0.05 were employed ( Ayres et al., 2007 ). The sample collections were conducted according to the System Authorization and Information on Biodiversity (SISBIO number 39329-1). ETHIC: The ethics committee on animal use (CEUA) at the Federal University of Espirito Santo approved this study under protocol number 53/2015. Results In retrospective study from total of 212 records 50.0 % turtles (106/212) were parasitized. According to the physical condition of the animals, the evaluation of body condition (body condition score poor, intermediate and good

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Seasonality and host-parasite interrelationship of Hysterothylacium aduncum (Nematoda) in whiting Merlangius merlangus off the southern and northern coasts of the Black Sea

based on Stern’s exact score limit, Mean I - Mean Intensity and Mean A - Mean Abundance with bootstrap 95 % Cl (Mean I, A), v/m – varlance-to-mean ratio of H. aduncum, SE – Standard Error Season/fish length/sex N Fish Length±SE Prevalence (%) Cl Mean I Cl Mean A Cl v/m k* Sinop Samples Seasons  Summer (June – August) (20.4 – 26.1 °C) 26 15.5 ±0.2 80.8 (61.7–92.1) 5.3 (3.2–9.1) 4.3 (2.5–7.5) 9.5 0.711  Autumn (September – November) (15.2 – 21.4 °C) 222 15.5 ±0.2 88.3 (83

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Phagocytosis in Mesocestoides vogae-induced peritoneal monocytes/macrophages via opsonin-dependent or independent pathways

twice, firstly to study phagocytosis of HEMA particles and secondly, the uptake of liposomes. Detailed protocols are described later. Peritoneal cells from pathogen-free ICR mice served as the naive control (N/0). Cell Staining PEC isolated from mice on selected days p.i. in the first experiment were used also for preparation of cell smears. Following fixation in 70 % methanol in PBS (pH 6.8), cells were stained with May-Grünwald/Giemsa stains according to the standard protocol. Leukocyte differential counts were determined by scoring 500 cells on each slide

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