Joanna Kochan, Wojciech Niżański, Nei Moreira, Zalmir Silvino Cubas, Agnieszka Nowak, Sylwia Prochowska, Agnieszka Partyka, Wiesława Młodawska and Józef Skotnicki
indigenous population of the lynx and European wildcat utilising in situ methods have been implemented in Poland for many years. In the case of lynx conservation, these professional activities in particular cover restoration and improvement of the connection between large forests, improvement of the forest quality, improvement of the availability of food sources, and increasing biodiversity and expanding existing populations through reintroduction ( 43 , 44 ). The areas inhabited by the European wildcat are usually protected as natural landscape parks or national parks
Yu-Xi Song, Pan Hu, Yun-Long Bai, Chang Zhao, Cheng Xia and Chuang Xu
temperature to dryness. The dry extract was then redissolved in 100 μL of acetonitrile:H 2 O (1:1, v/v), vortexed for 30 s, sonicated for 10 min in an ice bath, and centrifuged at 12,000 × g for 15 min at 4°C to remove insoluble debris. From each treated sample, 5 μL of the supernatant was mixed as a quality control (QC) sample and 70 μL was aliquoted for LC–MS detection ( 12 ).
Detection analysis . Detection was performed using an Agilent 1290 Infinity II LC system (Agilent Technologies, USA) coupled to a TripleTOF 6600 System (Q-TOF; AB Sciex, USA).
Jim Weber, Markus Zenker, Gábor Köller, Manfred Fürll and Markus Freick
(Serum-Kabevette, Kabe Labortechnik GmbH, Germany) were obtained from the coccygeal vein. Additionally, serum blood samples were also taken from control dairy cows (CDC) matched by housing group, lactation number, lactation stage, and gestation stage. Before the tubes were centrifuged (4 min, 3,000 × g), samples were allowed to coagulate for 30 min at 25°C in order to extract serum of high quality ( 17 ). Serum was subsequently stored at −20°C for a maximum of 14 d until analysis, as no marked changes in analyte levels are to be expected during short-term storage ( 4
Jacek Kijowski, Celina Marciszewska, Renata Cegielska-Radziejewska and Anna Popiół
, 33 , 83-91.
8. Hughey V.L., Johnson E.A.: Antimicrobial activity of lysozyme against bacteria involved in food spoilage and foodborne disease. Appl Environ Microbiol 1987, 53 , 2165-2170.
9. Jelle B.: Influence of a selected Lactobacillus strain on the shelf-lifeof fresh poultry meat. Prevention and control of potentially pathogenic microorganisms in poultry and poultry meat processing. Hygienic aspects of processed poultry meat. Proceedings of 13th European Symposium on the Qualityof Poultry Meat, Doorwerth, Netherlands, 1991
Iwona Puzio, Jose Luis Valverde Piedra, Małgorzata Kapica, Radosław P. Radzki, Marek Bieńko, Marta Pawłowska and Sylwia Szymańczyk
plant extracts and formic acid on the intestinal equilibrium of early-weaned pigs. J Anim Sci 2004, 82, 3210-3218.
17. Marzocco S., Piacente S., Pizza C., Oleszek W., Stochmal A., Pinto A., Sorrentino R., Autore G.: Inhibition of inducible nitric oxide synthase expression by yuccaol C from Yucca schidigera roezl. Life Sci 2004, 75, 1491-1501.
18. Matsumoto M., Sudo T., Saito T., Osada H., Tsujimoto M.: Involvement of p38 mitogen-activated protein kinase signaling pathway in osteoclastogenesis mediated by receptor activator of NF
disease are common in the population. Most oral lesions, especially chronic periodontitis, have no enough apparent pain to be alerted. Therefore, timely treatment is difficult. In the case of normal immune function, oral lesions exist for a long time without attack. Once the immune function changes, an acute attack may occur, even inducing infection during the treatment of certain systemic diseases. Thus, treatment plans for systemic diseases are difficult to proceed smoothly. This phenomenon affects the qualityoflifeof patients and endangers their entire health