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Open access

Goran Pavlisa, David Ozretic, Marko Rados and Gordana Pavlisa

Migration of Enterprise stent in treatment of intracranial aneurysms: a report of two cases

Background. We present two patients with acutely ruptured complex aneurysms of the internal carotid artery, arising at the origin of the posterior communicating artery (PComA).

Case reports. The aneurysms in both patients had a wide neck and the closed-cell stent (Enterprise) was delivered to assist in aneurysm coiling. In both patients an inadvertent migration of stent occurred, without periprocedural complications. Aneurysms were successfully embolized by endovascular coils.

Conclusions. These cases highlight the flexibility of the stent, as well as the likelihood of stent migration in the setting of immediate coiling after the placement of stent, or in adverse anatomic relations.

Open access

Lin Fang, Hai-Bing Zhang, Hua Li, Yong Fu and Guang-Shun Yang

miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90+ HepG2 cells

Background. Since the introduction of the theory of tumour stem cells (TSCs), the liver cancer stem cell (LCSC)-like cells have become one of the focuses in the research on liver cancer.

Materials and methods. In this study, CD90+ cells were applied as the possible LCSC-like cells, and the miRNA and gene expression were analyzed in the CD90+ HepG2 cells. The pilot study showed miR-548c-5p exerted potential effect on the CD90+ HepG2 cells and was thereafter applied for the further study. CD90+ HepG2 cells were assigned to miR-548c-5p mimic transfection group and control group. MTT assay was performed to detect the proliferation of CD90+ HepG2 cells. The migration and invasion abilities were examined by wound healing assay and transwell migration assay, respectively. A detection of apoptosis was performed by fluorescence microscopy.

Results. Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90+ HepG2 cells. Moreover, the miR-548c-5p transfection could down-regulate the expression of β-catenin, Tg737, bcl-2, bcl-XL, and caspase-3, inhibit the proliferation, migration and invasion and promote the apoptosis of the CD90+ HepG2 cells.

Conclusions. Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

Open access

George Fotopoulos, Ioannis Vathiotis, George C. Nikou and Konstantinos Syrigos

NETs and tumorigenesis is not well established but one could argue that the interaction with the homolog acting as tumor suppression is altered thus leading to tumorigenesis. In the same region lies the ZNF322A gene encoding a classical Cys2His2 zinc finger transcription factor. Although it has been associated with lung cancer formation, evidence remains controversial. ZNF322A gene upregulation boosted cell proliferation, migration and invasion. Furthermore, its knockdown diminished cell growth, invasion and also metastasis both in vitro and in vivo. Alpha

Open access

R. Sklavenitis-Pistofidis, T. Koletsa, A. Lazaridou and A. Goulas

-cell migration and adhesion.[ 21 , 22 ] Figure 2 B-cell Receptor (BCR) signalling pathway. Lyn, Syk: Src-family tyrosine kinases. PI3K: Phosphoinositide 3-kinase. PIP 2 : Phosphatidylinositol 4,5-biphosphate. PIP 3 : Phosphatidylinositol 3,4,5-triphosphate. BLNK: B-cell linker protein. PLCγ2: Phospholipase C γ-2. DAG: Diacylglycerol. PKC: Protein kinase C. NF-κB: Nuclear factor kappa B. BCR consists of a membrane immunoglobulin (mIg), noncovalently associated with Ig-α (CD79a) and Ig-β (CD79b), which contain immunoreceptor tyrosine-based activation motif

Open access

E. Veniou, I. Sofatzis, I. Kalantzis, M. Karakosta, M. Logothetis, E. Lianos and N. Ziras

breast and colon cancers. However, it is not clear if the antioxidant effect itself is sufficient to justify their anti-cancer properties. AGEs have a receptor (RAGE), belonging to the superfamily of immunoglobulins and can bind multiple ligands, including AGEs as well as amphoterin, an important protein involved in the regulation of inflammation, oncogenesis and cellular differentiation and migration. The receptor is expressed in many cancers such as colon, pancreatic and prostate cancer, but not in lung and esophageal cancer. Binding of AGEs causes genotoxic effect

Open access

Anastasios Kyriazoglou, Ioannis Dimitriadis and Aristotelis Bamias

Introduction Renal cell carcinoma (RCC) accounts for 2–3% of all new adult malignancies (1) . Incidental diagnosis because of the widespread use of abdominal imaging has led to a migration towards earlier stages at diagnosis, which are potentially curable (2) . Nevertheless, the incidence of all stages of RCC has increased over the past several years, contributing to a steadily increasing mortality rate per unit population. Our armamentarium of systemic therapy in RCC is rapidly improving. Novel agents targeting the vascular endothelial growth factor

Open access

Anastasios Kyriazoglou, Roubini Zakopoulou, Flora Zagouri, Aristotelis Bamias and Meletios Athanasios Dimopoulos

suppressor SMAD4, thus facilitating proliferation and migration of OS cells ( 57 ). 5 Hippo pathway and Drugs Hippo pathway deregulation has been implicated in resistance to chemotherapeutic drugs ( 58 ). Overexpression of YAP and TAZ in BRAF V600E mutant melanoma cells confers resistance to BRAF inhibitors ( 59 ). In BRAF V600E mutant lung cells YAP expression correlates with resistance to MEK and RAF inhibitors ( 60 ). TAZ is shown to mediate resistance to taxol in Breast cancer cells ( 61 ). Hippo pathway can be used as a target for therapeutic intervention

Open access

Vesna Todorovic, Gregor Sersa, Vid Mlakar, Damjan Glavac and Maja Cemazar

hypoxic cells and hypoxia-induced up-regulation of urokinase-type plasminogen activator receptor. Cancer Res 2004; 64 : 13-8. Goetze K, Scholz M, Taucher-Scholz G, Mueller-Klieser W. The impact of conventional and heavy ion irradiation on tumor cell migration in vitro. Int J Radiat Biol 2007; 83 : 889-96. Ogata T, Teshima T, Kagawa K, Hishikawa Y, Takahashi Y, Kawaguchi A, et al. Particle irradiation suppresses metastatic potential of cancer cells. Cancer Res 2005; 65 : 113-20. Wild

Open access

Tjasa Vizin and Janko Kos

in cancer cell migration and invasion. Biochim Biophys Acta 2007; 1773: 642-52. 77. Walsh JL, Keith TJ, Knull HR. Glycolytic enzyme interactions with tubulin and microtubules. Biochim Biophys Acta 1989; 999: 64-70. 78. Trojanowicz B, Winkler A, Hammje K, Chen Z, Sekulla C, Glanz D, et al. Retinoic acid-mediated down-regulation of ENO1/MBP-1 gene products caused decreased invasiveness of the follicular thyroid carcinoma cell lines. J Mol Endocrinol 2009; 42: 249-60. 79. Georges E, Bonneau AM, Prinos P. RNAi-mediated knockdown of alpha

Open access

Klemen Zupancic, Andrej Blejec, Ana Herman, Matija Veber, Urska Verbovsek, Marjan Korsic, Miomir Knezevic, Primoz Rozman, Tamara Lah Turnsek, Kristina Gruden and Helena Motaln


Background. Glioblastoma multiforme (GBM) is a brain tumour with a very high patient mortality rate, with a median survival of 47 weeks. This might be improved by the identification of novel diagnostic, prognostic and predictive therapy-response biomarkers, preferentially through the monitoring of the patient blood. The aim of this study was to define the impact of GBM in terms of alterations of the plasma protein levels in these patients. Materials and methods. We used a commercially available antibody array that includes 656 antibodies to analyse blood plasma samples from 17 healthy volunteers in comparison with 17 blood plasma samples from patients with GBM.

Results. We identified 11 plasma proteins that are statistically most strongly associated with the presence of GBM. These proteins belong to three functional signalling pathways: T-cell signalling and immune responses; cell adhesion and migration; and cell-cycle control and apoptosis. Thus, we can consider this identified set of proteins as potential diagnostic biomarker candidates for GBM. In addition, a set of 16 plasma proteins were significantly associated with the overall survival of these patients with GBM. Guanine nucleotide binding protein alpha (GNAO1) was associated with both GBM presence and survival of patients with GBM.

Conclusions. Antibody array analysis represents a useful tool for the screening of plasma samples for potential cancer biomarker candidates in small-scale exploratory experiments; however, clinical validation of these candidates requires their further evaluation in a larger study on an independent cohort of patients.