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Open access

Yifan Xu, Tianyu Lu, Wu Xu, Yuxiang Dai, Weibang Liang and Wei Jin

patients with colorectal cancer. Med Oncol. 2014;31(11):253. DOI: 10.1007/s12032-014-0253-8 4. Chen C, Zhao Z, Liu Y, Mu D. microRNA-99a is downregulated and promotes proliferation, migration and invasion in non-small cell lung cancer A549 and H1299 cells. Oncol Lett. 2015;9(3):1128-34. DOI: 10.3892/ol.2015.2873 5. Zhang J, Jin H, Liu H, Lv S, Wang B, Wang R, et al. MiRNA-99a directly regulates AGO2 through translational repression in hepatocellular carcinoma. Oncogenesis. 2014;3:e97. DOI: 10.1038/oncsis.2014.11 6. Feng Y

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Žanka Bojić-Trbojević, Nikola Kolundžić, Miloš Petronijević and Ljiljana Vićovac

human cytotro-phoblasts. J Steroid Biochem Mol Biol 1993; 46: 1-10. Guller S, Markiewicz L, Wozniak R, Burnham JM, Wang EY, Kaplan P, Lockwood CJ. Developmental regulation of glucocorticoid-mediated effects on extracellular matrix protein expression in the human placenta. Endocrinology 1994; 134: 2064-71. Moiseeva EP, Spring EL, Baron JH, De Bono DP. Galectin 1 modulates attachment, spreading and migration of cultured vascular smooth muscle cells via inter-actions with cellular receptors and components of

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Agnieszka Knopik-Skrocka, Patrycja Kręplewska and Donata Jarmołowska-Jurczyszyn

pathological neovascularization. Circul Res 1999; 85: 221-228. [4] Augsten M. Cancer-associated fibroblasts as another polarized cell type of the tumor microenvironment. Front Oncol 2014; 4: doi: 10.3389/fonc.2014.00062. [5] Ausprunk DH, Folkman J. Migration and proliferation of endothelial cells in preformed and newly formed blood vessels during tumor angiogenesis. Microvasc Res 1977; 14: 53-65. [6] Baeten C, Hillen F., Pauwels P, de Bruine A., Baeten C. Prognostic role of vasculogenic mimicry in colorectal cancer. Dis Colon

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Ana Parabucki, Anja Santrač, Danijela Savić, Sanja Dacić, Ivana Bjelobaba, Sanja Peković and Mirjana Stojiljković

regulation of chondroitin sulfate proteoglycans by astrocytes. Glia 2005; 52: 209-18. 11. Morrison B, Elkin BS, Dolle J-P, Yarmush ML. In vitro models of traumatic brain injury. Annu Rev Biomed Eng 2011; 13: 91-126. 12. Wu VW, Schwartz JP. Cell Culture Models for Reactive Gliosis: New Perspectives. J Neurosci Res 1998; 681: 675-81. 13. Liang C-C, Park AY, Guan J-L. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2007; 2: 329-33. 14. Robel S

Open access

David Goldberg and George Soleas

. Ethanol stimulates lipoprotein A-I secretion by human hepatocytes: implications for a mechanism for atherosclerosis protection. Metabolism 1992; 41: 827-32. Locher R, Suter PM, Vetter W. Ethanol suppresses smooth muscle cell proliferation in the post-prandial state: a new antiatherosclerotic mechanism of ethanol? Am J Clin Nutr 1998; 67: 338-41. Hendrickson RJ, Okada SS, Cahill PA, Sitzman JV, Redmond EM. Ethanol inhibits basal and flow-induced vascular smooth muscle cell migration in vitro. J Surg Res 1999; 84: 64

Open access

Olga A. Baturina, Alexey E. Tupikin, Tatyana V. Lukjanova, Svetlana V. Sosnitskaya and Igor V. Morozov

Summary

Background: Efficient treatment of inherited hyperphenyl-alaninemia requires exact identification of mutations defining the trait. Such knowledge is important both for effective individual therapy and understanding of the genetic history and evolution of regional populations.

Methods: DNA sequencing of amplified genome regions was used to identify mutations.

Results: Hyperphenylalaninemia-associated mutations in the phenylalanine hydroxylase locus were identified for 76 unrelated patients from the Novosibirsk region, Russia and for their family members. Twenty-one mutation types were identified, most of them rare and one (IVS2+1delG) not previously described. Common for European populations, the mutation p.R408W appeared to be the most frequent, with allele frequency 63.33%. We also looked for mutations in the quinoid dihydropteridine reductase locus in some patients. For 36 unrelated children PKU patients with known blood phenylalanine levels, we tried to find correlations between this level and the genotype.

Conclusions: Comparative analysis revealed correlations between blood phenylalanine levels and genotypes. The spectrum of phenylalanine hydroxylase mutations in the Novosibirsk region population appeared to be rather complex, probably as a result of mixed ethnic composition, formed by several multidirectional migration flows.

Open access

Lada Žrsković, Ninoslav Djelić, Vladan Bajićs, Nataša Bogavac-Stanojwić, Dijana Žukovec, Andrea Cabarkapa and Biljana Spremo-Potparevič

Summary

Background: The antioxidant activity of estrogen has a be ne - ficial impact in Alzheimer’s disease. A variety of clinical stu - dies have demonstrated that estrogen replacement therapy in postmenopausal women results in a lower frequency of AD, delaying the onset of the neurodegenerative cascade. On the other hand, it has been demonstrated that estrogens may exhibit genotoxic effects, especially at elevated tissue concentrations. Therefore, the aim of this study was to determine whether b-estradiol induces DNA damage in the peripheral blood lymphocytes of healthy young females and males, healthy elderly females and males and females and males with Alzheimer’s disease.

Methods: All experiments were performed using the alkaline version of the Comet assay (single cell gel electrophoresis), on six donors per each experimental group and controls.

Results: In the Comet assay, a significant increase of DNA migration was observed in the lymphocytes of all treated groups (young and elderly females, young and elderly males, AD females and AD males) at all b-estradiol concentrations (50 mmol/L, 100 mmol/L and 250 mmol/L) used in this investigation. In all the experiments cell viability was over 80%.

Conclusions: Lymphocytes are sensitive to the test concentrations of b-estradiol in the Comet assay regardless of gender, age and health condition of the examined subjects. Therefore, the role of b-estradiol in cellular DNA damage has been confirmed.

Open access

Mariusz J. Nawrocki, Joanna Budna, Piotr Celichowski, Ronza Khozmi, Artur Bryja, Wiesława Kranc, Sylwia Borys, Sylwia Ciesiółka, Sandra Knap, Michal Jeseta, Dorota Bukowska, Paweł Antosik, Klaus P. Brüssow, Małgorzata Bruska, Michał Nowicki, Maciej Zabel and Bartosz Kempisty

, Sumelka E, Jeseta M, Brüssow KP, Bukowska D, Antosik P, Bruska M, Nowicki M, Zabel M, Kempisty B. “Cell Migration” Is the Ontology Group Differentially Expressed in Porcine Oocytes Before and After In Vitro Maturation: A Microarray Approach. DNA Cell Biol. 2017;36(4):273-282. 5. Kranc W, Celichowski P, Budna J, Khozmi R, Bryja A, Ciesiółka S, Rybska M, Borys S, Jeseta M, Bukowska D, Antosik P, Brüssow KP, Bruska M, Nowicki M, Zabel M, Kempisty B. Positive regulation of macromolecule metabolic process belongs to the main mechanisms crucial for porcine oocytes

Open access

Lin Hua, Lin Li, Ping Zhou and Zheng Yang

Summary

Background: Coronary artery disease (CAD) is a complex trait influenced by genetic and environmental factors. Geographic isolation and natural selection present fundamental forces to diversify genetic backgrounds during human evolution and migration. In this study, we attempted to assess whether human geographic isolation affects the genetic predisposition of CAD.

Methods: We first included 21 genetic association studies of the methyl enetetrahydrofolate reductase (MTHFR) gene polymorphism C677T and CAD from 16 geographic regions consisting of 9,008 participants and performed a meta-ana - lysis based on the distributions of these studies.

ResultsIt was found that the positive signals for the association of C677T with CAD were mainly enriched in the regions of northern Afri ca (pooled OR=1.73, 95% CI=1.45-2.06, Z=3.17, P<0.0001) and India (pooled OR=1.61, 95% CI=1.30- 2.00, Z=4.38, P<0.0001). To validate the potential geographic effects on the genetic polymorphism of CAD, we then carried out two additional metaanalyses involving 30 and 13 studies on genetic associations of the angiotensin-converting enzyme (ACE) gene insertion/ deletion (I/D) and APOA5 gene T1131C polymorphisms for CAD consisting of 22,190 and 12,322 participants, respectively. We found that the associations of T1131C with CAD were concentrated in East Asia (pooled OR=1.35, 95% CI=1.22-1.49, Z=6.00, P<10-5), whereas the associations of I/D polymorphism with CAD were clustering in Europe and America (pooled OR=1.20, 95% CI=1.04-1.39, Z=2.49, P=0.01) and Turkey (pooled OR=1.33, 95% CI=1.05-1.69, Z=2.40, P=0.02).

Conclusions: Our results showed that geographic isolation might have potential effects on the genetic polymorphism of human complex diseases, such as CAD.

Open access

Roland Kaufmann, Franziska Mußbach, Annett Urbanek, Utz Settmacher and Ferdinand von Eggeling

cell activation. J Lab Clin Med 1992; 120: 513-19. 23. Sato Y, Kataoka H, Asada Y, Marutsuka K, Kamikubo Y, Koono M, Sumiyoshi A. Overexpression of tissue factor pathway inhibitor in aortic smooth muscle cells inhibits cell migration induced by tissue factor/factor VIIa complex. Thromb Res 1999; 94: 401-6. 24. Shah B, Shah G. Antifibrotic effect of heparin on liver fibrosis model in rats. World J Gastrointest Pharmacol Ther 2012; 3: 86-92. 25. Kaufmann R, Rahn S, Pollrich K, Hertel J, Dittmar Y, Hommann M, et al. Thrombin-mediated hepatocellular carcinoma cell