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Arunas Stankevičius, Jurate Buitkuviene, Jurgita Deltuvytiene, Ramune Čepuliene, Virginija Zilionyte, Ina Pampariene and Judita Žymantiene

). Act Vet Hung 2013, 61, 529-536. 11. ISO Committee on conformity assessment (CASCO). General requirements for the competence of testing and calibration laboratories (ISO/IEC 17025). 2005, 1-66. 12. Janutenaite J., Scerbavicius R., Blazevicius E.: Current status of porcine reproductive and respiratory syndrome in Lithuania, sero-epidemiological study by ELISA. Proc 5 th Inter Cong Vet Virol 2000, 244-245. 13. Kaden V., Lagen E., Hanel A., Hlinak A., Mewes L., Hergarten G., Irsch B., Deken J., Bruer.: Retrospective serological survey on selected viral pathogens

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Miodrag Dragoj, Jasna Bankovic, Ana Podolski-Renic, Sonja Stojkovic Buric, Milica Pesic, Nikola Tanic and Tijana Stankovic

, initially under low-stringency conditions and then at high-stringency conditions, to generate informative fingerprints of moderate complexity that could distinguish tumor from normal tissue. The reactions were performed in a GeneAmp® PCR System 9700 (Applied Biosystems, Foster City, CA, USA) using PCR reaction kits supplied by Fermentas Life Sciences (Lithuania). Each reaction consisted of an initial denaturation step (95 °C for 5 min), 4 cycles at low-stringency conditions (specific for each primer), 35 cycles at high-stringency conditions (specific for each primer), and

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Vaida Petrauskiene, Ruta Vaiciuniene, Vytautas Kuzminskis, Edita Ziginskiene, Saulius Grazulis, Egle Jonaitiene, Erika Skrodeniene and Inga Arune Bumblyte

Abstract

Background and objectives: Vascular calcification (VC) is one of the factors associated with mortality in hemodialysis (HD) patients. The purpose of the study was to assess associations between prevalent VC and disturbances of calcium-phosphate metabolism as well as changes in vitamin D (25(OH)D), FGF 23 and MGP levels and to evaluate the possible impact of VC and changes of these biomarkers on survival in HD patients.

Methods: The study population consisted of 81 prevalent patients in the hemodialysis unit of Hospital of Lithuanian University of Health Sciences Kaunas Clinics. A simple vascular calcification score (SVCS) was evaluated as it is described by Adragao et al. 25(OH)D (nmol/L), FGF 23 (ng/L) and MGP (ng/mL) were measured and analysed.

Results: Patients were divided into two groups: SVCS<3 (31 patient (38.3%) and SVCS ≥3 (50 patients (61.7%)). In multivariate logistic regression, age (odds ratio 1.062, 95% CI [1.024-1.1] p=0.001) and diabetes (odds ratio 6.9, 95% CI [1.5-31], p=0.012) were associated with SVCS ≥3. The multivariate logistic regression revealed the highest negative impact of SVCS ≥3, age and 25(OH)D level for death risk.

Conclusion: VC in HD patients is highly influenced by age and presence of diabetes and associated with higher risk of death. No significant association was found between MGP and FGF 23 and VC as well as between these two biomarkers and risk of death. Lower 25(OH)D levels were associated with mortality in this dialysis patients cohort.

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Grzegorz Woźniakowski, Magdalena Frączyk, Krzysztof Niemczuk and Zygmunt Pejsak

Abstract

African swine fever (ASF) is currently one of the most severe viral infections of domestic pigs, wild boars, and other hosts belonging to Suidae family. ASF is also considered as the most complex and devastating infectious and haemorrhagic disease of swine due to its severe socio-economic impact and transboundary character. ASF it is a notifiable disease and due to the lack of specific treatment and vaccine, the disease can be only limited by the administrative measures comprising wild boar hunting and stamping out of affected pigs. ASF occurred for the first time in Kenya in 1921 while in Europe (Portugal) the virus was detected at the end of the 1950s. In spite of successful eradication of this threat in a number of affected regions, the virus remains endemic in both feral and domestic pigs in Africa and Sardinia. The ‘new era’ of ASF started in 2007 after its re-introduction to Georgia. Following its intensive expansion, the virus spread to other Caucasian countries, including the territory of the Russian Federation. In 2014 the virus reached Ukraine, Belarus, and, consequently, European Union countries: Lithuania, Latvia, Estonia, and Poland. The occurrence of ASF in wild boars and pigs had a severe impact on both epidemiology and economy because of the national and international transport and trade consequences. Up to date, starting from the February 2014, eighty ASF cases in wild boar and three outbreaks in domestic pigs have been diagnosed. Taking into account the diverse rate of spread in Poland, this review aims to present and discuss the current state of knowledge on ASF including its epidemiology, pathology, transmission, and perspectives of control.

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Darius Danusevicius, G. Masaitis and G. Mozgeris

. Remote Sensing of Environment 30: 271-278. DANUSEVIC˘IUS, D. and R. GARBRILAVIC˘IUS (2001): Variation in juvenile growth rhythm among Picea abies provenances from the Baltic states and adjacent regions. Scandinavian Journal of Forest Research 16: 305-317. DANUSEVIC˘IUS, D., J. BUCHOVSKA, V. STANYS, J. B. S˘ IKS˘NIANIENE, V. MAROZAS and V. BENDOKAS (2013): DNA marker based identification of spontaneous hybrids between Pinus mugo and P. sylvestris at the Lithuanian sea-side. Nordic Journal of Botany 31: 1-9. DATT, B. (1998

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Ewa Bilska-Zając, Mirosław Różycki, Ewa Chmurzyńska, Jacek Karamon, Jacek Sroka, Ewelina Antolak, Marek Próchniak and Tomasz Cencek

Lithuania. In: Molecular Epidemiology of Trichinellosis in Lithuania and Biological characteristics of Trichinella genotypes in Rat . PhD Thesis. Copenhagen 2002, p. 135. 13. Merialdi G., Bardasi L., Fontana M.C., Spaggiari B., Maioli G., Conedera G., Vio D., Londero M., Marucci G., Ludovisi A., Pozio E., Capelli G.: First reports of Trichinella pseudospiralis in wild boars ( Sus scrofa ) of Italy. Vet Parasitol 2011, 178, 370–373. 14. Moskwa B., Goździk K., Bień J., Borecka A., Gawor J., Cabaj W.: First report of Trichinella pseudospiralis in Poland in

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Lina Merkeviciene, Neda Ruzauskaite, Irena Klimiene, Rita Siugzdiniene, Jurgita Dailidaviciene, Marius Virgailis, Raimundas Mockeliunas and Modestas Ruzauskas

22531. 14. Ruzauskas M., Couto N., Kerziene S., Siugzdiniene R., Klimiene I., Virgailis M., Pomba C.: Prevalence, species distribution and antimicrobial resistance patterns of methicillin-resistant staphylococci in Lithuanian pet animals. Acta Vet Scand 2015, 57, 27, doi: 10.1186/s13028-015-0117-z. 15. Ruzauskas M., Siugzdiniene R., Butrimaite-Ambrozeviciene C., Zymantiene J., Klimiene I., Vaskeviciute L., Mockeliunas R., Virgailis M.: Prevalence and characterization of multi-resistant Staphylococcus spp., isolated from poultry liver. J Food Safety 2016

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Dominika Guzek, Dominika Głąbska, Paweł Plewa, Karolina Kozań, Jacek Pietras, Rafał Plewa, Ewelina Pogorzelska, Grzegorz Pogorzelski, Jędrzej Trajer and Agnieszka Wierzbicka

Pap Rep 2011, 29 , 89-97. 10. Polkinghorne R., Thompson J.M., Watson R., Gee A., Porter M.: Evolution of the Meat Standards Australia (MSA) beef grading system. Aust J Exp Agric 2008, 48 , 1351-1359. 11. Razmait V., Kerzien S., Jatkauskien V., Nainien R., Urbšien D.: Pork quality of male hybrids from Lithuanian Wattle pigs and wild boar intercross. Agron Res 2009, 7 , 47-58. 12. Resurreccion A.V.A.: Sensory aspects of consumer choices for meat and meat products. Meat Sci 2003, 66 , 11-20. 13. Sitz B

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Ewa Cisak, Angelina Wójcik-Fatla, Jacek Sroka, Violetta Zając, Ewa Bilska-Zając, Ewa Chmurzyńska and Jacek Dutkiewicz

., Juricová Z., Svobodová S., Halouzka J.: A serologic survey for some bacterial and viral zoonoses in game animals in the Czech Republic. J Wildl Dis 1993, 29 , 604-607. 9. Juceviciene A., Zygutiene M., Leinikki P., Brummer- Korvenkontio H., Salminen M., Han X., Vapalahti O.: Tick-borne encephalitis virus infections in Lithuanian domestic animals and ticks. Scand J Infect Dis 2005, 37 , 742-746. 10. Kańtoch M.: Tick-borne encephalitis - etiopathogenesis and implications for public health in Poland. Postępy Hig Med Dosw 1998, 52 , 3

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Jacek Karamon, Małgorzata Samorek-Pieróg, Bożena Moskwa, Mirosław Różycki, Ewa Bilska-Zając, Jolanta Zdybel and Magdalena Włodarczyk

helminth parasites of the small intestine in red foxes from central Poland. Med Weter 2009, 65, 33–35. 7. Bruzinskaite-Schmidhalter R., Sarkunas M., Malakauskas A., Mathis A., Torgerson P.R., Deplazes P.: Helminths of red foxes ( Vulpes vulpes ) and raccoon dogs ( Nyctereutes procyonoides ) in Lithuania. Parasitology 2012, 139, 120–127. 8. Davidson R.K., Gjerde B., Vikoren T., Lillehaug A., Handeland K.: Prevalence of Trichinella larvae and extra-intestinal nematodes in Norwegian red foxes ( Vulpes vulpes ). Vet Parasitol 2006, 136, 307–316. 9. Eckert