Ikpa Chinyere Benardette Chinaka, Onoja Samuel Okwudili and Dozie-Akaniro Ijeoma Nkiru
The study investigated the bioactive constituents, antioxidant and antibacterial activities of Platycerium bifucartum leaves. Chloroform fraction of P. bifurcatum was prepared by partitioning the ethanol extract with chloroform and water. The phytochemical analysis was carried out using standard methods. Fourier transformer-infrared (FT-IR) and Gas chromatography-mass spectroscopy (GC/MS) were used in the characterization of the bioactive compounds. The antioxidant and antibacterial activities of the chloroform fraction were evaluated using standard protocols. The fourier transformer-infrared analysis showed the presence of C=O, OH, CHO, C-F and -NH functional groups. GC/MS characterization gave benzeneethaneamine (33.3%), 2-amino-1-(4-methylphenyl) propane (17.04%), hydroxyurea (30.26%) and epinephrine (13.26). The extract inhibited the growth of the bacterial isolates. The fraction exhibited antioxidant properties that were comparable with ascorbic acid. The presence of these compounds showed that the leaves of Platycerium bifurcatum can be used for the treatment of some bacterial diseases.
Introduction: The nutritive value and terapeuthic activity of watermelon seeds is known, but up to day no studies on isolation and characterisation of their melanin were conducted.
Objective: The aim of the study was to evaluate the antioxidant, antibacterial and light barrier properties of raw and purified melanins isolated from watermelon seeds.
Methods: Native melanin was isolated from seeds by alkaline extraction. Obtained pigment was purified by acid hydrolysis. Chemical tests and FT-IR analysis were conducted to determine the melanin nature of the isolated pigments. UV-Vis, transmittance and colour properties were evaluated spectrophotometrically. Antioxidant activity was determined using ABTS and antibacterial activity through a well diffusion method.
Results: The results of the study demonstrated that melanins isolated from watermelon seeds had antioxidant, light barrier and antibacterial properties. A purified form of melanin had higher antioxidant activity and light barrier properties than the raw form. Both melanins inhibited the growth of Enterococcus faecalis and Pseudomonas aeruginosa.
Conclusions: Watermelon seeds may be considered as a promising source of natural melanin which possess remarkable therapeutic action that can support the traditional use of this plant in the ethnomedicine.
Justyna Bauza-Kaszewska, Krzysztof Skowron, Zbigniew Paluszak, Zbigniew Dobrzański and Mścisław Śrutek
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