on mercury chloride induced changes on the spleen of adult wistar rats. J Exp Clin Anatomy 13 : 60–65.
Jacob S, Thangarajan S. (2017). Effect of gestational intake of fisetin (3,3’,4’,7-Tetrahydroxyflavone) on developmental methyl mercury neurotoxicity in F1 generation rats. Biol Trace Elem Res 177 : 297–315.
Jagadeesan G, Kavitha AV, Subashini J. (2005). FT-IR Study of the influence of Tribulus terrestris on mercury intoxicated mice, Mus musculus liver. Trop Biomed 22 : 15–22.
Jagadeesan G, Kavitha AV. (2006). Recovery of phosphatase
Ljubiša Đorđević, Stevo Najman, Perica Vasiljević, Miroslav Miljković, Nenad Ignjatović, Dragan Uskoković and Milenko Plavšić
V, Najman S, Plavšić M, Uskoković D. A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy. Biomaterials 2001; 22(6): 571-575.
13. Najman S, Đorđević Lj, Savić V, Ignjatović N, Plavšić M, Uskoković D. Changes of HAp/PLLA biocomposites and tissue reaction after subcutaneous implantation. Facta Universitatis Series: Medicine and Biology 2003; 10(3): 131-134.
14. Najman S, Savic V, Djordjevic Lj. Ignjatovic N, Uskokovic D. Biological evaluation of hydroxyapatite/poly-L-lactide (HAp/PLLA) composite
Hajnal Kelemen, Gabriel Hancu, Serban Andrei Gâz-Florea, Eniko Nemes-Nagy, Lajos Attila Papp and Eleonora Mircia
Macrocycl Chem. 2016;84(3-4):189-196.
20. Wang JH, Cai Z – Investigation of inclusion complex of miconazole nitrate with β-cyclodextrin. Carbohydrate polymers. 2008;72(2): 255-260.
21. Gupta HKK – Solid state compatibility studies of miconazole using thermal and spectroscopic methods. Adv Anal Chem. 2015;5(3):51-55.
22. Barillaro V, Dive G, Ziémons et al. – Theoretical and experimental vibrational study of miconazole and its dimers with organic acids: Application to the IR characterization of its inclusion complexes with cyclodextrins. . Int J Pharm
Gordana D. Radosavljevic, Jelena Pantic, Ivan Jovanovic, Miodrag L. Lukic and Nebojsa Arsenijevic
1. Liu FT, Rabinovich GA. (2005). Galectins as modulators of tumour progression. Nat Rev Cancer, 5(1), 29-41.
2. Radosavljevic G, Volarevic V, Jovanovic I, Milovanovic M, Pejnovic N, Arsenijevic N, et al. (2012). The roles of Galectin-3 in autoimmunity and tumor progression. Immunol Res, 52,100–110.
3. Moutsatsos IK, Wade M, Schindler M, Wang JL. (1987). Endogenous lectins from cultured cells: nuclear localization of carbohydrate binding protein 35 in proliferating 3T3 fibroblasts. Proc Natl Acad Sci USA, 84, 6452–6456.
4. Yu F
Slavoljub C. Živanović, Ružica S. Nikolić and Goran M. Nikolić
21. Erdogan G, Karadg R. Quercetin (3,3’,4’,5,7- pentahydroxyflavone) complexes with calcium(II) and magnesium(II), its potentiometric and spectrophotometric studies. Rev Anal Chem 2005; 24: 9-23. http://dx.doi.org/10.1515/REVAC.2005.24.1.9
22. Paczkowska M, Lewandowska K, Bednarski W et al. Application of spectroscopic methods for the identification (FT-IR, Raman spectroscopy) and determination (UV, EPR) of quercetin-3-Orutinoside. Experimental and DFT based approach. Spectrochim Acta A 2015; 140
Petra Šimić, Zlatko Giljević, Velimir Šimunić, Slobodan Vukičević and Mirko Koršić
Abu-Amer Y, Tondravi MM. NFkappaB and bone: the breaking point. Nat Med 1997;3:1189-90.
Garrett IR, Chen D, Gutierrez G, Zhao M, Escobedo A, Rossini G, Harris SE, Gallwitz W, Kim KB, Hu S, Crews CM, Mundy GR. Selective inhibitors of the osteoblast proteasome stimulate bone formation in vivo and in vitro . J Clin Invest 2003;111:1771-82.
Tremollieres FA, Pouilles JM, Ribot C. Vertebral postmenopausal bone loss is reduced in overweight women: a longitudinal study in 155 early postmenopausal women. J Clin
Jozo Grgic, Filip Sabol, Sandro Venier, Jason Tallis, Brad J. Schoenfeld, Juan Del Coso and Pavle Mikulic
optimal for everyone? Inter-individual variation in caffeine ergogenicity, and a move towards personalised sports nutrition Sports Med 2018 48 1 7 16
Pickering C, Kiely J. What should we do about habitual caffeine use in athletes? Sports Med , 2019; 49(6): 833– 42 30173351 10.1007/s40279-018-0980-7
Pickering C Kiely J What should we do about habitual caffeine use in athletes? Sports Med 2019 49 6 833 42
Pickering C. Caffeine, CYP1A2 genotype, and sports performance: is timing important? Ir J Med Sci , 2019; 188(1): 349–50 29633162
Ikpa Chinyere Benardette Chinaka, Onoja Samuel Okwudili and Dozie-Akaniro Ijeoma Nkiru
The study investigated the bioactive constituents, antioxidant and antibacterial activities of Platycerium bifucartum leaves. Chloroform fraction of P. bifurcatum was prepared by partitioning the ethanol extract with chloroform and water. The phytochemical analysis was carried out using standard methods. Fourier transformer-infrared (FT-IR) and Gas chromatography-mass spectroscopy (GC/MS) were used in the characterization of the bioactive compounds. The antioxidant and antibacterial activities of the chloroform fraction were evaluated using standard protocols. The fourier transformer-infrared analysis showed the presence of C=O, OH, CHO, C-F and -NH functional groups. GC/MS characterization gave benzeneethaneamine (33.3%), 2-amino-1-(4-methylphenyl) propane (17.04%), hydroxyurea (30.26%) and epinephrine (13.26). The extract inhibited the growth of the bacterial isolates. The fraction exhibited antioxidant properties that were comparable with ascorbic acid. The presence of these compounds showed that the leaves of Platycerium bifurcatum can be used for the treatment of some bacterial diseases.
Introduction: The nutritive value and terapeuthic activity of watermelon seeds is known, but up to day no studies on isolation and characterisation of their melanin were conducted.
Objective: The aim of the study was to evaluate the antioxidant, antibacterial and light barrier properties of raw and purified melanins isolated from watermelon seeds.
Methods: Native melanin was isolated from seeds by alkaline extraction. Obtained pigment was purified by acid hydrolysis. Chemical tests and FT-IR analysis were conducted to determine the melanin nature of the isolated pigments. UV-Vis, transmittance and colour properties were evaluated spectrophotometrically. Antioxidant activity was determined using ABTS and antibacterial activity through a well diffusion method.
Results: The results of the study demonstrated that melanins isolated from watermelon seeds had antioxidant, light barrier and antibacterial properties. A purified form of melanin had higher antioxidant activity and light barrier properties than the raw form. Both melanins inhibited the growth of Enterococcus faecalis and Pseudomonas aeruginosa.
Conclusions: Watermelon seeds may be considered as a promising source of natural melanin which possess remarkable therapeutic action that can support the traditional use of this plant in the ethnomedicine.
Piotr Brodzki, Adam Brodzki, Łukasz Kurek, Jan Marczuk and Marcin R. Tatara
Bartolome JA., Silvestre F.T., Kamimura S., Arteche A.C.M., Melendez P. K e l bert D., McHale J., Swift K., Archbald L.F.,Thatcher W.W. (2005). Resynchroniza tion of ovulation and timed insemination in lactating dairy cows: I: use of the Ovsynch and Heatsynch protocols after non-pregnancy diagnosis by ultrasonography Theriogenology, 63: 1617-1627.
Binelli M., Machado R. Bergamaschi M.A.C.M., Bertan C.M. (2009). Manipulation of ovarian and uterine function to increase conception rates in cattle. Anim. Reprod. 6: 125