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Comparison of methods for the identification and sub-typing of O157 and non-O157 Escherichia coli serotypes and their integration into a polyphasic taxonomy approach

bacteria to utilise 95 various carbon sources and assigns unknown strains to a species by mathematically comparing their utilisation pattern (metabolic fingerprint) with the Biolog database ( Odumeru et al ., 1999 ; Kootallur et al ., 2011 ). Fourier transform-infrared (FT-IR) spectroscopy is considered a chemotaxonomic method that is able to provide a great amount of taxonomic data. Spectra obtained from cellular biomass are very characteristic and can be considered as ‘fingerprints’ or molecular patterns distinctive of a particular bacterial strain ( Naumann, 2000

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Application of Fourier Transform Infra-red Evolved Gas Analysis (FT-IR-EGA) to the Study of Tobacco Curing

References 1. Lephardt, J. O., and R. A. Fenner: Characterization of pyrolysis and combustion of complex systems using Fourier transform infrared spectroscopy; Appl. Spectrosc. 34 (1980) 174—185. 2. Lephardt, J, O., and R. A. Fenner: Fourier transform infrared evolved gas analysis — Additional considerations and options; Appl. Spectrosc. 35 (1980) 95—101. 3. Lephardt, J. O.: Fourier transform infrared evolved gas analysis (FT-IR-EGA); Appl, Spectrosc. Rev. 18 (1982-3) 265

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Milk acetone determination by the photometrical method after microdiffusion and via FT infra-red spectroscopy

, Ficnar J, Jílek M, Ticháček A, Jedelská R, Dolínková A (1999): A notes to interpretation procedures of results ketone and acetone contents evaluation (Ketotest) in individual cow's milk samples (in Czech). Cattle Research 61: 17-34. Hanuš O, Genčurová V, Kopecký J, Jedelská R, Motyčka Z, Černocký M (2008a): The interpretation of experimental calibration results of routine IR instruments for determination of free fatty acid contents of milk fat (in Czech). Cattle Research 50: 55-62. Hanuš O, Hering P, Frelich J, Jílek

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An Application of Fourier Transform Infra-red (FT-IR) Microspectroscopy to the Study of Cigarette Manufacturing Defects


FT-IR microspectroscopy was used to investigate a common type of cigarette defect in which the filter separates from the tobacco rod. Infra-red imagings of the adhesive located at this junction on the tipping papers from both defective and acceptable cigarettes were obtained. A comparison of these data revealed that although adhesive was present in the seam area of the defective cigarettes, the amount of adhesive was significantly less and its distribution was not uniform.

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Influence of Soil Traits on Polyphenols Level in Moltkia petraea (Tratt.) Griseb. (Boraginaceae)

. H., 2007: An alternative method to measure carbonate in soils by FT-IR spectroscopy. Environmental Chemistry Letters 5, 9–12. Tavarini, S., Sgherri, C., Ranieri, A. M., Angelini, L. G., 2015: Effect of nitrogen fertilization and harvest time on steviol glycosides, flavonoid composition and antioxidant properties in Stevia rebaudiana Bertoni. Journal of Agriculture and Food Chem istry 63, 7041–7050. Young, J. E., Zhao, X., Carey, E. E., Welti, R., Yang, S. S., Wang, W., 2005: Phytochemical phenolic in organically grown vegetables. Molecular Nutrition

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Kinetic Profile of the Antioxidant Activity of Propolis Extract: 2,2-Diphenyl-1-Picrylhydrazyl Radical Bleaching Assay

profiles, FT-IR and UV–vis spectroscopic data. Journal of Food Composition & Analysis, 24 , 516-522. Neves, M.V.M.D., Silva, T.M.S.D., Oliveira Lima, E.D., Cunha, E.V.L.D,. Oliveira, E.D.L. (2016). Isoflavone formononetin from red propolis acts as a fungicide against Candida sp. Brazilian Journal of Microbiology , 47 , 159-166. Potkonjak, N.I., Veselinović, D.S., Novaković, M.M., Gorjanović, S.Ž., Pezo, L.L., Sužnjević, D.Ž. (2012). Antioxidant activity of propolis

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A methodological framework to determine optimum durations for the construction of soil water characteristic curves using centrifugation

, Ostrode, Germany), with an 11150 model four-bucket rotor and bespoke adaptors designed to ft within the centrifuge buckets. The bespoke adaptors ( Figure 1 ) were designed to facilitate the use of 5 × 5 cm ( Peerlkamp and Boekel, 1960 ; Reatto et al ., 2008 ; Moncada et al ., 2015 ) ρ b rings commonly used in the field ( Creamer, 2015 ). An Acculab Atilon ATL2202 balance (Sartorius, Goettingen, Germany), with a precision of 0.01 g was used to weigh the soil cores at specified intervals. During the structured experiment, the following pressure steps were applied

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Occurrence of chemicals with known or suspected endocrine disrupting activity in drinking water, groundwater and surface water, Austria 2017/2018

triiodothyronine (T3) to thyroid hormone receptors (TRs) and suppress T3 actions; b) In vivo, bisphenol A can impair thyroid hormone action by antagonizing T3-induced TR activation (TRα1 and TRβ1) and by suppressing its transcriptional activity in a dose-dependent manner; c) Triclosan affects thyroid hormone-dependent metamorphosis in animals; d) Urinary phthalates are cross-sectionally associated with lower free thyroxine (fT4) and higher thyroid stimulating hormone (TSH); e) PFASs interfere with binding of thyroid hormone to transthyretin and upregulate deiodinase in the

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Comparison of Chemical Structure of Alginite Humic Acids Isolated with Two Different Procedures with Soil Humic Acids

Science Publishers, Inc., pp. 123. ISBN 978-1-61668-965-0. MALCOLM, M.L. 1990. The uniqueness of humic substances in each of soil stream and marine environments. In Analytica Chimica Acta , vol. 232 , pp.19‒30. MAO, J. ‒ OLK, D.C. ‒ FANG, X. ‒ HE, Z. ‒ SCHMIDT-ROHR, K. 2008. Influence of animal manure applicatioín on the chemical structures of soil organic mater as investigated by advanced solid-state NMR and Ft-IR spectroscopy. In Geoderma , vol. 146 , no. 1‒2, pp. 353‒362. ONO, K. ‒ HIRAI, K

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Antioxidant, antibacterial properties and the light barrier assessment of raw and purified melanins isolated from Citrullus lanatus (watermelon) seeds


Introduction: The nutritive value and terapeuthic activity of watermelon seeds is known, but up to day no studies on isolation and characterisation of their melanin were conducted.

Objective: The aim of the study was to evaluate the antioxidant, antibacterial and light barrier properties of raw and purified melanins isolated from watermelon seeds.

Methods: Native melanin was isolated from seeds by alkaline extraction. Obtained pigment was purified by acid hydrolysis. Chemical tests and FT-IR analysis were conducted to determine the melanin nature of the isolated pigments. UV-Vis, transmittance and colour properties were evaluated spectrophotometrically. Antioxidant activity was determined using ABTS and antibacterial activity through a well diffusion method.

Results: The results of the study demonstrated that melanins isolated from watermelon seeds had antioxidant, light barrier and antibacterial properties. A purified form of melanin had higher antioxidant activity and light barrier properties than the raw form. Both melanins inhibited the growth of Enterococcus faecalis and Pseudomonas aeruginosa.

Conclusions: Watermelon seeds may be considered as a promising source of natural melanin which possess remarkable therapeutic action that can support the traditional use of this plant in the ethnomedicine.

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