Kibrom M. Alula, James H. Resau and Osman V. Patel
quantification. An overlay image ( a ) was unmixed into blue hematoxylin ( b ) and red VDR ( c ). The unmixed images of dual-labeled slide enable quantification of individual, as well as overlapping signals within the same outlined lobular border. The scale bar represents 50 mm (10× magnification) in the photomicrographs. VDR, vitamin D receptor.
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impurities in the crystallization experiments, were labeled with the fluorescent dyes Alexa Fluor® 488 TFP ester and Alexa Fluor® 594 NHS ester, respectively. The Alexa Fluor® 488 TFP ester (C 39 H 44 F 4 N 4 O 11 S 2 , M W = 884.9 Da) and Alexa Fluor® 594 NHS ester (C 39 H 37 N 3 O 13 S 2 , M W = 819.8 Da) were obtained from Thermo Fisher Scientific (Life Technologies). The Alexa Fluor® 488 TFP ester (5 mg) was dissolved in 0.5 mL of dimethyl sulfoxide. The reactive dye solution (80 μL) was slowly added to the stirring Pf GST tetramer solution (10 mg/ml in PBS). The
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Michał Gęca, Konrad Pietrykowski and Karol Rosiński
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