Kibrom M. Alula, James H. Resau and Osman V. Patel
quantification. An overlay image ( a ) was unmixed into blue hematoxylin ( b ) and red VDR ( c ). The unmixed images of dual-labeled slide enable quantification of individual, as well as overlapping signals within the same outlined lobular border. The scale bar represents 50 mm (10× magnification) in the photomicrographs. VDR, vitamin D receptor.
Statistical analyses within (HG and SC: G20 vs P1; G20 vs P3; P1 vs P3 stages) a group and between (HG vs SC within G20; P1; P3) groups were done using Student’s t -test (GraphPad Prism ® 6.07). Data
After sacrifice, C57BL/6 (B6) mice spleens were aseptically removed and placed in Roswell Park Memorial Institute (RPMI) 1640 medium. Spleens were smashed through sterile nylon mesh followed by red blood cell ammonium-chloride-potassium (ACK) lysing bufer. Then cells were re-suspended in RPMI 1640 supplemented with l-glutamine (2 mM), penicillin (100 U/mL), streptomycin (0.1 mg/mL), 2-ME (5 x 10 −5 M), and heat-inactivated fetal bovine serum (FBS) (10%). Given many T-cells needed, several spleens were pooled together to set up T
Arayik Martirosyan, Lawrence J. DeLucas, Christina Schmidt, Markus Perbandt, Deborah McCombs, Martin Cox, Christopher Radka and Christian Betzel
impurities in the crystallization experiments, were labeled with the fluorescent dyes Alexa Fluor® 488 TFP ester and Alexa Fluor® 594 NHS ester, respectively. The Alexa Fluor® 488 TFP ester (C 39 H 44 F 4 N 4 O 11 S 2 , M W = 884.9 Da) and Alexa Fluor® 594 NHS ester (C 39 H 37 N 3 O 13 S 2 , M W = 819.8 Da) were obtained from Thermo Fisher Scientific (Life Technologies). The Alexa Fluor® 488 TFP ester (5 mg) was dissolved in 0.5 mL of dimethyl sulfoxide. The reactive dye solution (80 μL) was slowly added to the stirring Pf GST tetramer solution (10 mg/ml in PBS). The
Pedro J. Llanos, Kristina Andrijauskaite, Vijay V. Duraisamy, Francisco Pastrana, Erik L. Seedhouse, Sathya Gangadharan, Leonid Bunegin and Mariel Rico
temperature environment was more important than having the T-cells in the proper gas mix due to the extreme cold temperatures in Van Horn (–5–0°C).
On the L–1 d (December 10, 2017), the team estimated the new mass of the payload to be 441 g (initial mass of NanoLab was 498 g), which was less than that anticipated in previous feasibility studies ( Vela et al., 2017 ). This met the mass requirement of <500 g. NanoRacks provided polycarbonate standoffs placed on the outside of the NanoLab, which is a customer-developed payload package with dimensions of 10.16 cm × 10.16 cm
L. Chen, E.A. Selimovic, M. Daunis, T.A. Bayers T, L.J. Vargas, I.T. O’Brien, C.B. McEnroe, A.E. Kozerski, A.C. Vanhoover, W.D. Gray and J.F. Caruso
added to the 1.0 kg IET sled. Knee extension was done with a velcro cuff around their distal left shank. As the knee extended ~10–15°, the sled traveled rapidly to the end of the track. As it traveled, the knee flexed back to its initial joint angle. Before the sled reached the end of the track, the next repetition occurred, which accelerated the sled to the track’s opposite end. These high-speed movements were repeated over successive repetitions until subjects were proficient in the exercise. Changes in sled direction created an impact force, which was high due to