Hai-Feng Liu, Hui Li, Ge Bai, Qian-Zhen Zhang, Xiang-Gao, Tao Liu and Hong-Bin Wang
(WBC) and red blood cell (RBC) counts and was performed immediately after sample collection with an MEK-7222K haematology analyser (Nihon Kohden Corporation, Japan). Serum was stored at −80°C after centrifugation.
A total of 300 mg of xylocaine was injected intravenously for euthanasia after general anaesthesia with xylazine and ketamine hydrochloride at 13 weeks, or earlier if a serious complication occurred.
Histopathological examination. Tissue was collected from the caudal pole of the remnant kidney. The renal tissue was fixed in 10% buffered formalin
1. Centre for Science and Environment, 2014: Antibiotics in Chicken Meat: study . New Delhi: Pollution Monitoring Laboratory, 36 p. PML/PR-48/2014.
2. Cháfer-Pericás, C., Maquieira, Á., Puchades, R., 2010: Fast screening methods to detect antibiotic residues in food samples. Trends in Analytical chemistry , 29, 9, 1038—1049. DOI:10.1016/j.trac.2010.06.004.
3. European Commission, 2002: Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the
J. Novotný, P. Reichel, K. Bárdová, P. Kyzeková and V. Almášiová
1. Alexopoulos, C., Papaioannou, D. S., Fortomaris, P., Kyriakis, C. S., Tserveni-Goussi, A., Yannakopoulos, A., Kyriakis, S. C., 2007: Experimental study on the effect of in-feed administration of a clinoptilolite-rich tuff on certain biochemical and haematological parameters of growing and fattening pigs. Livest. Sci. , 111, 230—241. DOI: 10.1016/j.livsci.2007.01.152.
2. Ball, R. O., Aherne, F. X., 1982: Effect of diet complexity and feed restriction on the incidence and severity of diarrhoea in early-weaned pigs. Can. J. Anim. Sci
Kai K. Komine Y. Komine K. Asai K. Kuroishi T. Kozutsumi T. Itagaki M. Ohta M. Kumagai K. Effects of bovine lactoferrin by the intramammary infusion in cows with staphylococcal mastitis during the early non-lactating period J Vet Med Sci 2002 64 873 878
9 Lasagno M., Ortiz M., Vissio C., Yaciuk R., Bonetto C., Pellegrino M., Bogni C., Odierno L., Raspanti C.: Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes Microb Pathog 2018, 116, 146–152. 10.1016/j
Sample collection and analysis . Blood samples of each cow were collected daily before morning feeding by venepuncture of the caudal vein, and heparin sodium was used as an anticoagulant. The samples were immediately centrifuged at 1,400 g for 10 min. The plasma was subsequently stored at −80°C. Blood parameters, including BHBA, nonesterified fatty acids (NEFA), glucose (Glu), calcium (Ca), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), triglycerides (TG), total bilirubin (TBIL), and γ-glutamyl transpeptidase (GGT) were
25.79 ± 2.42 b
29.46 ± 0.82 b
42.27 ± 1.16 a
42.39 ± 2.46 a
GST (U/mg protein)
23.19 ± 0.84 a
21.05 ± 0.80 ab
14.96 ± 0.84 c
13.87 ± 1.02 c
21.03 ± 0.90 ab
19.97 ± 1.19 b
SOD (U/g protein)
77.49 ± 0.25 a
77.19 ± 0.31 ab
74.18 ± 0.22 c
73.00 ± 0.58 c
76.10 ± 0.27 ab
75.83 ± 0.81 b
The data are expressed as mean ± SE for seven animals per group. Within rows, means with different letters (a, b, and c) are significantly different (p < 0.001)
Plasma AST, ALT, ALP, and LDH activities and cholesterol levels
, and tolfenamic acid were tested at concentrations of 0.05, 0.1, 0.5, 1, 5, 10, and 20 μg/ml, while carprofen and ketoprofen assay concentrations were the same and additionally 40μg/ml. The concentrations in which these compounds were tested were established based on those in the literature ( 20 ), the maximum concentrations they reach in canine serum, and the permissible maximum non-cytotoxic solvent concentration. As a positive control, non-treated cells were used, and as a negative control, cells were challenged with doxorubicin at a concentration of 0.5 μg
Natalia Mazur-Panasiuk, Jacek Żmudzki and Grzegorz Woźniakowski
uptake of flies fed ASFV-infected blood.
ASFV resistance and stability have attracted the interest of numerous investigators over the years ( 5 , 7 , 10 , 23 , 26 , 27 , 28 , 41 , 42 , 47 ). It has been proved that ASFV shows high resistance to environmental conditions and remains infectious over a long storage time either below 0°C or at 4°C. The curing process of infected meat (a process like that which Parma, Iberian, or Serrano ham undergoes) facilitated survival of ASFV in ham for over a year ( 28 ). ASFV can survive many freeze–thaw cycles, and