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Oxytetracycline Residues in Honey Analyzed by Liquid Chromatography with UV Detection

classification regarding maximum residue limits in foodstuffs of animal origin. Off. J. Eur. Commun. 2010, L 15, pp. 1-72. Dinkov D., Kanelov I., Zhelyazkova I., Vashin I. (2005) - Persistence of tetracycline and oxytetracycline in bee honey after improper application on bee families. Bulg. J. Vet. Med ., 8(3): 205-509. European Medicines Agency Veterinary Medicines and Inspections: Summary opinion of the committee for medicinal products for veterinary use on the establishment of Maximum Residue Limits - Oxytetracycline (Extension to honey

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ISOLATION, IDENTIFICATION AND ANTIBIOTIC RESISTANCE OF AEROMONAS SPP. AND SALMONELLA SPP. FROM THE FRESH WATER LOACH, LEPIDOCEPHALICHTHYS GUNTEA AND WATER OF TERAI RIVER LOTCHKA, WEST BENGAL, INDIA

Abstract

An investigation was conducted to identify the bacterial isolates and to study the antibiotic resistance patterns of Aeromonas spp. and Salmonella spp. from the skin, gills, and gut of the fresh water loach, Lepidocephalichthys guntea (HAMILTON BUCHANAN) and water sampled from four different sites along the River Lotchka in Darjeeling District, West Bengal, India. Isolated bacteria were identified by different biochemical procedures, and Polymerase Chain Reaction was performed using genus specific 16S rDNA primers for confirmation of identification of Aeromonas spp. and Salmonella spp. Antibiotic susceptibility test of bacterial isolates was also done by Disc Diffusion method. A total of 49 Aeromonas spp. and 24 Salmonella spp. were isolated from tested samples. Maximum resistance was exhibited for Penicillin-G, Ampicillin, and Cephalothin (>75% resistant Aeromonas spp. and >60% Salmonella spp. in the four different sites of river and body parts of fish). Ciprofloxacin and Tetracycline resistance was almost nil. The results indicated that the river water and resident fish were contaminated with multi-antibiotic resistant enteric pathogenic bacteria. This study, thus, provides valuable information for making policy decisions aimed at reducing microbial contamination of fish and the indiscriminate use of antibiotics.

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The genetic approach for assessing sea trout stock enhancement efficiency – An example from the Vistula River

Atlantic salmon ( Salmo salar ) caught in the Baltic Sea – ICES J. Mar. Sci. 63: 1274-1285. Kołder W. 1958 – Stocking salmon and sea trout into the upper reaches of the Vistula River system in the years 1879-1954 – Roczn. Nauk Rol. B-73: 216-267 (in Polish). Krumme U., Bingel F. 2016 – Tetracycline marks visible in Baltic cod Gadus morhua otoliths stored for 40 years – J. Fish Biol. 89: 2189-2194. Marshall T.C., Slate J., Kruuk L.E.B., Pemberton J.M. 1998 – Statistical confidence for likelihood-based paternity inference in natural populations – Mol

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Effects of Lactobacillus Johnsonii AJ5 Metabolites on Nutrition, Nosema Ceranae Development and Performance of Apis Mellifera L.

. Probiotics and Antimicrobial Proteins, 4, 39-46. Simion, G., Trif, A., Cara, M. C. & Damiescu, L. (2011). Evaluation of tetracyclines’ and cloramphenicol’s residues levels in honey from Timis County between 2007 and 2010. (1), 264-269. Smart, M., & Sheppard, M. (2012). Nosema ceranae in age cohorts of the western honey bee (Apis mellifera). Journal of Invertebrate Pathology, 109, 148-151. Undeen, A. H., & Vávra, J. (1997). Research methods for entomopathogenic Protozoa. Manual of Techniques in Insect Pathology. Academic Press

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Improving disease prevention and treatment in controlled fish culture

-115. Harnisz M., Zmysłowska I., Gołaś I., Terech-Majewska E. 2004 – Occurrence of Gram-negative rods in water and fish during intense fattening – In: Current Challenges in Fish Disease Prevention and Treatment (Eds) A.K. Siwicki, J. Antychowicz, W. Szweda, Wyd. IRS, Olsztyn: 131-136 (in Polish). Harnisz M., Gołaś I., Pietruk M. 2011 – Tetracycline-resistant bacteria as indicators of antimicrobial resistance in protected waters – The example of the Drwęca River Nature Reserve (Poland) – Ecol. Ind. 11: 663-668. Harnisz M. 2013 – Total resistance of native bacteria

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Antimicrobial Susceptibility of Streptococci Most Frequently Isolated from Czech Dairy Cows with Mastitis

References Aminov R.I., Garrigues-Jeanjean N., Mackie R.I. (2001). Molecular ecology of tetracycline resistance: development and validation of primers for detection of tetracycline resistance genes encoding ribosomal protection proteins. Appl. Environ. Microbiol., 67: 22–32. Bengtsson B., Ericsson Unnerstad H., Ekman T., Artursson K., Nilsson-Ost M., Persson Waller K. (2009). Antimicrobial susceptibility of udder pathogens from cases of acute clinical mastitis in dairy cows. Vet. Microbiol., 136: 142–149. Bradley A.J. (2002). Bovine mastitis: an

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Preliminary study on serological, molecular, histological and clinical evidence of Chlamydia spp. in Polish swine herds

., Donati M., Rossi M., Pignanelli S., Shurdhi A., Baldelli R., Cevenini R. (2008). Tetracycline-resistant Chlamydia suis isolates in Italy. Vet. Rec., 163: 251-252. Eggemann G., Wendt M., Hoelzle L.E., Jaeger C., Weiss R., Falling K. (2000). Prevalence of Chlamydia infections in breeding sows and their importance in reproductive failure. Deutsch. Tierärztl. Wschr., 107: 3-10. Englund, S.,af Segerstad C.H., Amlund F., Westergren E., Jacobson M. (2012). The occurrence of Chlamydia spp. in pigs with and without clinical disease. Vet. Res., 26

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Determining Influence of Culture Media and Dose-Dependent Supplementation with Basic Fibroblast Growth Factor on the Ex Vivo Proliferative Activity of Domestic Cat Dermal Fibroblasts in Terms of Their Suitability for Cell Banking and Somatic Cell Cloning of Felids

.M., Garnick J.J., Newhouse R., Sharawy M.M. (1998). Human periodontal ligament fibroblast response to PDGF-BB and IGF-1 application on tetracycline HCI conditioned root surfaces. J. Clin Periodontol., 25: 404–412. Giraldo A.M., Hylan D.A., Ballard C.B., Purpera M.N., Vaught T.D., Lynn J.W., Godke R.A., Bondioli K.R. (2008). Effect of epigenetic modifications of donor somatic cells on the subsequent chromatin remodeling of cloned bovine embryos. Biol. Reprod., 78: 832–840. Gómez M.C., Pope C.E. (2015). Cloning endangered felids by interspecies somatic cell

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