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Gender- and age-specific reference intervals of common biochemical analytes in chinese population – derivation using real laboratory data

temperature fluctuations in thyroid-stimulating hormone. Clin Biochem 2018; 60: 59–63. 30130522 10.1016/j.clinbiochem.2018.08.008 Wang D Cheng X Yu S Qiu L Lian X Guo X et al Data mining: Seasonal and temperature fluctuations in thyroid-stimulating hormone Clin Biochem 2018 60 59 63 10 Li D, Wang D, Wang D, Ma C, Wu J, Li P, et al. Data mining: Biological and temporal factors associated with blood cardiac troponin I concentration in a Chinese population. Clin Chim Acta 2019; 495: 8–12. 10.1016/j.cca.2019.03.1628 Li D Wang D

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Serum Level of HMGB1 Protein and Inflammatory Markers in Patients with Secondary Peritonitis: Time Course and the Association with Clinical Status

Summary

Background: Intra-abdominal infection in secondary peritonitis drives as excessive production of inflammatory mediators and the development of systemic inflammatory response syndrome (SIRS) or sepsis. Finding a specific marker to distinguish SIRS from sepsis would be of immense clinical importance for the therapeutic approach. It is assumed that high-mobility group box 1 protein (HMGB1) could be such a marker. In this study, we examined the time course changes in the blood levels of HMGB1, C-reactive protein (CRP), procalcitonin (PCT) and serum amyloid A (SAA) in patients with secondary peritonitis who developed SIRS or sepsis.

Methods: In our study, we evaluated 100 patients with diffuse secondary peritonitis who developed SIRS or sepsis (SIRS and SEPSIS group) and 30 patients with inguinal hernia as a control group. Serum levels of HMGB1, CRP, PCT, and SAA were determined on admission in all the patients, and monitored daily in patients with peritonitis until discharge from hospital.

Results: Preoperative HMGB1, CRP, PCT and SAA levels were statistically highly significantly increased in patients with peritonitis compared to patients with inguinal hernia, and significantly higher in patients with sepsis compared to those with SIRS. All four inflammatory markers changed significantly during the follow-up. It is interesting that the patterns of change of HMGB1 and SAA over time were distinctive for SIRS and SEPSIS groups.

Conclusions: HMGB1 and SAA temporal patterns might be useful in distinguishing sepsis from noninfectious SIRS in secondary peritonitis.

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The Esr Test: An Old Test With New Contents

The Esr Test: An Old Test With New Contents

The erythrocyte sedimentation rate (ESR) remains one of the most widely used laboratory tests. Its clinical usefulness and interpretation are in the monitoring of inflammatory diseases, in particular rheumatoid arthritis, temporal arteritis and polymyalgia rheumatica. At present, the reference method for measuring the ESR proposed by the International Committee for Standardization in Haematology (ICSH) utilizes EDTA-anticoagulated-undiluted blood to perform the test using the method described by Westergren in 1921. Current interest in the methodology focuses on the development of an automated closed system that allows the determination of the sedimentation rate with selected working methods, using a single sample for more than one haematological test, improving the bio-hazardous aspects of the testing procedures. As a consequence, standardization becomes necessary. ESR results should be reliable, despite the increased number of different methods and testing variables. Control materials and External Quality Assurance Schemes are now available, and should be used. In conclusion, innovative techniques may improve the appropriateness and usefulness of ESR in clinical practice, but in addition, they need to guarantee the traceability of results in comparison to the reference method in order to ensure comparability of results among different clinical laboratories.

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Madge - Microplate Array Diagonal Gel Electrophoresis

Madge - Microplate Array Diagonal Gel Electrophoresis

Microplate array diagonal gel electrophoresis (MADGE) was invented for molecular genetic epidemio-logical studies. MADGE is a highly flexible, cost effective, microplate compatible solution to high throughput electrophoresis needs. It enables several thousands to million gel lines per day for direct assay of single-base variation in different capacity laboratories. Variants of the standard 96-well MADGE include: 96-well stretch-MADGE, 192-well MADGE, 384-well MADGE, and 768-well MADGE. Melt-MADGE combines the temporal thermal ramp apparatus to achieve similar throughput for de novo mutation scanning. Basic MADGE principles and procedures, preparation of MADGE gels, electrophoresis, visualization and analysis of these gels, as well as modifications of the basic 96-well MADGE will be discussed in detail. For the first time in our country, this revolutionary polyacrylamid electrophoresis was done in 1998. We shortly review our studies which used MADGE for high throughput genotyping of the apolipoprotein E. MADGE and melt-MADGE will have an important role in the future genetic research of complex diseases and especially in pharmacogenomics.

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Inhibition of Cholesterol Biosynthesis in Hypercholesterolemia – Is It the Right Choice? / Inhibicija Biosinteze Holesterola u Hiperholesterolemiji – Da Li Je Pravi Izbor?

: quantitative and temporal aspects. J Cell Physiol 1988; 137: 133-40. 16. Naderi S, Blomhoff R, Myklebust J, et al. Lovastatin inhibits G1/S transition of normal human B-lymphocytes independent of apoptosis. Exp Cell Res 1999; 252: 144-53. 17. Kabakoff BD, Doyle JW, Kandutsch AA. Relationships among dolichyl phosphate, glycoprotein synthesis, and cell culture growth. Arch Biochem Biophys 1990; 276: 382-9. 18. McCarty MF. Suppression of dolichol synthesis with isoprenoids and statins may potentiate the cancer-retardant efficacy

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Spermine and L-Name Pretreatment Effects on Polyamine and Nitric Oxide Metabolism in Rat Brain During Seizures

distribution of polyamines in temporal lobe epilepsy. Epilepsy Res 1999; 35: 161-72. Gilad GM, Gilad VH. Polyamine biosynthesis is required for survival of sympathetic neurons after axonal injury. Brain Res 1983; 273: 191-4. Coert BA, Anderson RE, Meyer FB. Exogenous spermine reduces ischemic damage in a model of focal cerebral ischemia in the rat. Neurosci Lett 2000; 282: 5-8. Navaro-Gonzalvez JA, Garcia-Benayas C, Arenas J. Semiautomated measurement of nitrate in biological fluids. Clin

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Effect of Conventional and More Aggressive Rosuvastatin Treatment on Markers of Endothelial Activation

JB, Ghaisas N, Murphy R, Crean P, Walsh M. Early temporal expression of soluble cellular adhesion molecules in patients with unstable angina and subendocardial myocardial infarction. Am J Cardiol 1999; 83: 1265-7. Postadzhiyan A, Tzontcheva A, Kehayov I, Finkov B. Circulating soluble adhesion molecules ICAM-1 and VCAM-1 and their association with clinical outcome, troponin T and C-reactive protein in patients with acute coronary syndromes Clinical Biochemistry 2008; 41: 126-33. Hwang SJ, Ballantyne CM, Sharrett AR

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Increased Lymphocyte Caspase-3 Activity in Patients with Schizophrenia

, Catts SV, McGrath JJ, Feron F, McLean D, Coulson EJ, Lutze-Mann LH. Apoptosis and schizophrenia: a pilot study based on dermal fibroblast cell lines. Schizophr Res 2006; 84: 20-8. Jarskog LF, Selinger ES, Lieberman JA, Gilmore JH. Apoptotic proteins in the temporal cortex in schizophrenia: high Bax/Bcl-2 ratio without caspase-3 activation. Am J Psychiatry 2004; 161: 109-15. Tošić M, Ott J, Barral S, Bovet P, Deppen P, Gheorghita F, Matthey ML, Parnas J, Preisig M, Saraga M, Solida A, Timm S, Wang AG, Werge T, Cuenod

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Association Between Thyroid Hormones, Lipids and Oxidative Stress Markers in Subclinical Hypothyroidism / Povezanost Izme\U Tireoidnih Hormona, Lipida I Markera Oksidativnog Stresa U SubkliniĉKoj Hipotireozi

dysfunction on lipid profile. Open Cardiovasc Med J 2011; 5: 76-84. 10. Venditti P, Meo SD. Thyroid hormone-induced oxidative stress. Cell Mol Life Sci 2006; 63(4): 414-34. 11. Chattopadhyay S, Sahoo DK, Roy A, Samanta L, Chainy GB. Thiol redox status critically influences mitochondrial response to thyroid hormone-induced hepatic oxidative injury: A temporal analysis. Cell Biochem Funct 2010; 28: 126-34. 12. Fernandez V, Tapia G, Varela P, Romanque P, Cartier- Ugarte D, Videla LA. Thyroid hormone-induced oxidative stress in

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Ghrelin, Nitrite and Paraoxonase/Arylesterase Concentrations in Cement Plant Workers

-8. Barim AO, Aydin S, Colak R, Dag E, Deniz O, Sahin I. Ghrelin, paraoxonase and arylesterase levels in depressive patients before and after citalopram treatment. Clin Biochem 2009; 42: 1076-81. Furlong CE, Li WF, Richter RJ, Shih DM, Lusis AJ, Alleva E, et al. Genetic and temporal determinations of pesticide sensitivity: role of paraoxonase (PON1). Neuro-Toxicology 2000; 21: 91-100. Beaumont NJ, Skinner VO, Tan TM, Ramesh BS, Byrne DJ, MacColl GS, et al. Ghrelin can bind to a species of high density lipoprotein

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