Jernej Vidmar, Franci Bajd, Zoran V. Milosevic, Igor J. Kocijancic, Miran Jeromel and Igor Sersa
. With improvements of MRI technology, it can be expected that MRI scanning of patients with stroke could complement or even replace standard CT scanning in future. Therefore, MR-measurable parameters of thrombi, such as those presented in this study and perhaps some new ones, could in future, in combination with clinical and CT data help improving stroke treatment planning and easing treatment decisions. Results from this ongoing study my potentially influence further evolution of thrombectomy devices.
This study was financially supported by
Qing-Qing Chang, Chun-Yan Chen, Zhao Chen and Shuai Chang
(Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions.
Cell colony formation assay
After transfection, cells were seeded in 6-well plates at a density of 1×10 3 cells/well. The cells were then fixed and stained with 0.1% crystal violet after incubation for 14 days. The numbers of colonies were counted under microscope. This assay was performed at least three times.
Wound healing assays
Cells were incubated in 6-well plates at a density of 1 × 10 6 cells/well. After transfection, the cells were cultured with serum-free medium for
Jerneja Varl, Urska Ivanus, Ziva Pohar Marinsek, Tine Jerman, Anja Ostrbenk Valencak, Mario Poljak and Veronika Kloboves Prevodnik
papillomavirus testing and liquid-based cytology: results at recruitment from the new technologies for cervical cancer randomized controlled trial. J Natl Cancer Inst 2006; 98: 765-74. doi: 10.1093/jnci/djj209 16757701 10.1093/jnci/djj209
Ronco G Segnan N Giorgi-Rossi P Zappa M Casadei GP Carozzi F et al Human papillomavirus testing and liquid-based cytology: results at recruitment from the new technologies for cervical cancer randomized controlled trial J Natl Cancer Inst 2006 98 765 74 10.1093/jnci/djj209
23 Ronco G, Giorgi
Ming-Jun Fan, Shu-Mei Liang, Peng-Juan He, Xing-Bo Zhao, Ming-Jiang Li and Feng Geng
migrated through the membranes were counted under the inverted light microscope at 10x magnification. All tests were performed in triplicate.
RNA extraction and RT-qPCR
To verify the expression of DUSP6 in transfected cell, total RNA was isolated with TRIzol reagent (Life Technologies, USA) and converted to first-strand cDNA using M-MLV Reverse Transcriptase (USB, Cleveland, OH). The mRNA level was analyzed with quantitative real-time PCR assay. Specific primers were synthesized as the following sequences show: DUSP (forward) 5’-GAACTGTGGTGTCTTGGTACATT-3′ and
Vincenza Granata, Roberta Fusco, Sergio Venanzio Setola, Elisabetta de Lutio di Castelguidone, Luigi Camera, Salvatore Tafuto, Antonio Avallone, Andrea Belli, Paola Incollingo, Raffaele Palaia, Francesco Izzo and Antonella Petrillo
), are helpful for differentiating G2 NET from G1 ones. 65
Recently, the term of “Radiomics” has been introduced to define a mathematical process to extract innumerable quantitative features from medical images (including each diagnostic technique) with high-throughput computing for diagnosis and prediction. 66 Compared to traditional visual interpretation of medical images, the deep mining of medical images by computer technology from radiomics makes features uptake more efficient, relatively objective and rich in features types. Radiomics is promising for tumor
Mojca Jensterle, Soncka Jazbinsek, Roman Bosnjak, Mara Popovic, Lorna Zadravec Zaletel, Tina Vipotnik Vesnaver, Barbara Faganel Kotnik and Primoz Kotnik
craniopharyngioma have focused on minimizing treatment-related toxicity. These advances include endoscopic surgery and precision radiotherapy. In the last decades radiation therapy technology has improved dose conformality and provided decreased doses to adjacent critical structures (hypothalamus, optic tract, pituitary gland, carotid arteries, medial temporal lobe structures, etc.) with the goal of reducing long-term sequelae, especially endocrinologic and visual ones. Conformal RT enables a better coverage of the tumor while preserving surrounding tissue therefore decreasing
1. Tian M, Ajay VS, Dunzhu D, et al. A Cluster-Randomized, Controlled Trial of a Simplified Multifaceted Management Program for Individuals at High Cardiovascular Risk (SimCard Trial) in Rural Tibet, China, and Haryana, India. Circulation. 2015;132:815-824. doi:10.1161/CIRCULATIONAHA.115.015373.
2. Bohavnani SP, Narula J, Sengupta PP. Mobile technology and the digitalization of healthcare. Eur Heart J. 2016;37:1428-1438. https://doi.org/10.1093/eurheartj/ehv770 .
3. Abraham WT, Adamson PB, Bourge RC, et al. Wireless pulmonary artery
Background. Radiofrequency ablation (RFA) is a curative therapy for hepatocellular carcinoma (HCC). In RFA, ultrasonography (US) is most commonly used to guide tumor puncture, while its effects are assessed using dynamic computed tomography or magnetic resonance. The differences in modalities used for RFA and assessment of its effects complicate RFA. We developed a method for assessing the effects of RFA on HCC by combining contrast-enhanced (CE) US and real-time virtual sonography with three-dimensional US data.
Patients and methods. Before RFA, we performed a sweep scan of the target HCC nodule and the surrounding hepatic parenchyma to generate three-dimensional US data. After RFA, we synchronized multi-planar reconstruction images derived from stored three-dimensional US data with real-time US images on the same US monitor and performed CEUS and real-time virtual sonography. Using a marking function, we drew a sphere marker along the target HCC nodule contour on pre-treatment US- multi-planar reconstruction images so that the automatically synchronized sphere marker represented the original HCC nodule contour on post-treatment real-time CEUS images. Ablation was considered sufficient when an avascular area with a margin of several millimeters in all directions surrounded the sphere marker on CEUS.
Results. This method was feasible and useful for assessing therapeutic effects in 13 consecutive patients with HCC who underwent RFA. In 2 patients who underwent multiple sessions of RFA, HCC-nodule portions requiring additional RFA were easily identified on US images.
Conclusions. This method using advanced US technologies will facilitate assessment of the effects of RFA on HCC.