Esin Avci, Süleyman Demir, Diler Aslan, Rukiye Nar and Hande Şenol
Standardization Program (VDSP) ( 10 ) is an international effort collaborated with many constitutions etc. Central Disease Center (CDC), National Institute of Standards and Technology (NIST), NHANES, Belgian Laboratory for Analytical Chemistry, Faculty of Pharmaceutical Sciences, Ghent University (11). One of the objectives of VSDP is standardize 25-OH D assays with NIST traceable measurement procedures ( 12 ). Standard reference materials (SRM) 2972 and 972 has been recommended for improving traceability and harmonization of 25-OH vitamin D measurement ( 10 , 12 ).
the current evidence. Biochem Med (Zagreb) 2014; 24: 6.
4. Foord AG, Gulland WG. Can Technology Eliminate Human Error? Process Saf Environ Prot 2006; 84: 171-3.
5. Oren E, Shaffer ER, Guglielmo BJ. Impact of emerging technologies on medication errors and adverse drug events. Am J Health Syst Pharm 2003; 60: 1447-58.
6. Lippi G, Simundic AM, Mattiuzzi C. Overview on patient safety in healthcare and laboratory diagnostics. Biochem Med (Zagreb) 2010; 20: 131-43.
7. Simundic AM, Lippi G. Preanalytical phase
Agata Chamier-Gliszczyńska, Sandra Kałużna, Katarzyna Stefańska, Piotr Celichowski, Paweł Antosik, Dorota Bukowska, Małgorzata Bruska, Jana Zakova, Marie Machatkova, Michal Jeseta and Michał Nowicki
were then removed by vortexing the BCB+ oocytes in 1% sodium citrate buffer followed by mechanical displacement using a small-diameter glass micropipette. Only the GC-free BCB+ oocytes were used for subsequent IVM and microarray analysis.
IVM of porcine COCs
After the first BCB test, the COCs with stained blue cytoplasm (BCB+) were cultured in Nunclon™Δ four-well dishes in 500 mL of standard porcine IVM culture medium TCM-199 (tissue culture medium) with Earle’s salts and l-glutamine (Gibco BRL Life Technologies, Grand Island, NY) supplemented with 2.2 mg
Karel Crha, Pavel Ventruba, Jana Žáková, Michal Ješeta, Radovan Pilka, Jan Vodička and Igor Crha
epithelial cells, mucosal functions and physiological barrier against colonization by pathogenic bacteria. Bacterial colonization also significantly contributes to modulation of the host’s immunity. Metagenomic analyses have opened an extensive research of physiological colonization of human organism and demonstrated presence of microbes in areas that were originally considered to be absolutely sterile. The original dogma of the ”sterile uterus“ was overcome with the new knowledge. Significant limits of the research in this field were sample collection and technologies for
Krzysztof Janowicz, Paul Mozdziak, Artur Bryja, Bartosz Kempisty and Marta Dyszkiewicz-Konwińska
source for transplants [ 3 ]. Lately, bioprinting technology successfully produced three dimensional dentin pulp complex that not only contained vascular networks and cancellous bone, but indeed is patient specific, as opposed to commonly used artificial dental implants [ 9 ].
Proliferation, differentiation and mineralization
At the beginning of the millennium little was known about the differentiation capacity of dental pulp stem cells, despite researchers had extensive knowledge of how to stimulate growth of mesenchymal tissue for the sake of promoting tooth
Greg Hutchings, Mariusz J. Nawrocki, Paul Mozdziak and Bartosz Kempisty
analysis together suggested a site-specific induction of CM cell cycle re-entry, keeping the potential low for off-site pathogenic effects such as unintended hyperplasia [ 23 ]. Previous attempts to induce re-entry of CMs into the cell cycle have resulted in heart dysfunction. Notably, the hydrogel technology holds promise as a new delivery method for donor stem cells [ 24 ].
Although the results of this experiment highlight a potential treatment for ischaemia, it is important to note that the number of new cardiomyocytes formed is still too low to effectively replace
Katarzyna Stefańska, Ievgenia Kocherova, Sandra Knap, Magdalena Kulus, Piotr Celichowski and Michal Jeseta
determined from the optical density at 260 nm, and the RNA purity was estimated using the 260/280 nm absorption ratio (higher than 1.8) (NanoDrop spectrophotometer, Thermo Scientific, ALAB, Poland). The RNA integrity and quality were checked on a Bioanalyzer 2100 (Agilent Technologies, Inc., Santa Clara, CA, USA). The resulting RNA integrity numbers (RINs) were between 8.5 and 10 with an average of 9.2 (Agilent Technologies, Inc., Santa Clara, CA, USA). The RNA in each sample was diluted to a concentration of 100 ng/μl with an OD260/OD280 ratio of 1.8/2.0. From each RNA
Önsel Öner, Figen Deveci, Selda Telo, Mutlu Kuluöztürk and Mehmet Balin
Diagnostics Inc., Tarrytown, USA). Serum BNP and TnI concentrations were performed on the ADVIA Centaur XP immunoassay analyzer (Siemens Healthcare Diagnostics Inc., Tarrytown, NY). Plasma D-dimer levels were assessed by using the BCS XP coagulation analyzer (Siemens Healthcare Diagnostics, Marburg, Germany).
Serum MR-proADM and MR-proANP levels were measured with a commercially available kit using an ELISA, Human MR-proADM ELISA kit (Catalogue No: 201-12-7275 Sunred Biological Technology Co. Ltd, Shanghai) with a low sensitivity limit of 2.839 pg/mL. The samples were