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Assessment of abbott architect 25-oh vitamin D assay in different levels of vitamin D

Standardization Program (VDSP) ( 10 ) is an international effort collaborated with many constitutions etc. Central Disease Center (CDC), National Institute of Standards and Technology (NIST), NHANES, Belgian Laboratory for Analytical Chemistry, Faculty of Pharmaceutical Sciences, Ghent University (11). One of the objectives of VSDP is standardize 25-OH D assays with NIST traceable measurement procedures ( 12 ). Standard reference materials (SRM) 2972 and 972 has been recommended for improving traceability and harmonization of 25-OH vitamin D measurement ( 10 , 12 ). Recently

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Novel Opportunities for Improving the Quality of Preanalytical Phase. A Glimpse to the Future?

the current evidence. Biochem Med (Zagreb) 2014; 24: 6. 4. Foord AG, Gulland WG. Can Technology Eliminate Human Error? Process Saf Environ Prot 2006; 84: 171-3. 5. Oren E, Shaffer ER, Guglielmo BJ. Impact of emerging technologies on medication errors and adverse drug events. Am J Health Syst Pharm 2003; 60: 1447-58. 6. Lippi G, Simundic AM, Mattiuzzi C. Overview on patient safety in healthcare and laboratory diagnostics. Biochem Med (Zagreb) 2010; 20: 131-43. 7. Simundic AM, Lippi G. Preanalytical phase

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Medical Journal of Cell Biology
The Journal of Foundation for Cell Biology and Molecular Biology
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Journal of Medical Biochemistry
The Journal of Society of Medical Biochemists of Serbia
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Analysis of expression of genes responsible for regulation of cellular proliferation and migration – microarray approach based on porcine oocyte model

were then removed by vortexing the BCB+ oocytes in 1% sodium citrate buffer followed by mechanical displacement using a small-diameter glass micropipette. Only the GC-free BCB+ oocytes were used for subsequent IVM and microarray analysis. IVM of porcine COCs After the first BCB test, the COCs with stained blue cytoplasm (BCB+) were cultured in Nunclon™Δ four-well dishes in 500 mL of standard porcine IVM culture medium TCM-199 (tissue culture medium) with Earle’s salts and l-glutamine (Gibco BRL Life Technologies, Grand Island, NY) supplemented with 2.2 mg

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The role of uterine microbiome and epithelial-mesenchymal transition in endometrial function

epithelial cells, mucosal functions and physiological barrier against colonization by pathogenic bacteria. Bacterial colonization also significantly contributes to modulation of the host’s immunity. Metagenomic analyses have opened an extensive research of physiological colonization of human organism and demonstrated presence of microbes in areas that were originally considered to be absolutely sterile. The original dogma of the ”sterile uterus“ was overcome with the new knowledge. Significant limits of the research in this field were sample collection and technologies for

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Human Dental Pulp Stem Cells: recent findings and current research

source for transplants [ 3 ]. Lately, bioprinting technology successfully produced three dimensional dentin pulp complex that not only contained vascular networks and cancellous bone, but indeed is patient specific, as opposed to commonly used artificial dental implants [ 9 ]. Proliferation, differentiation and mineralization At the beginning of the millennium little was known about the differentiation capacity of dental pulp stem cells, despite researchers had extensive knowledge of how to stimulate growth of mesenchymal tissue for the sake of promoting tooth

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Cardiac Stem Cell Therapy, Resident Progenitor Cells and the role of Cellular Signalling; a Review

analysis together suggested a site-specific induction of CM cell cycle re-entry, keeping the potential low for off-site pathogenic effects such as unintended hyperplasia [ 23 ]. Previous attempts to induce re-entry of CMs into the cell cycle have resulted in heart dysfunction. Notably, the hydrogel technology holds promise as a new delivery method for donor stem cells [ 24 ]. Although the results of this experiment highlight a potential treatment for ischaemia, it is important to note that the number of new cardiomyocytes formed is still too low to effectively replace

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The genes regulating maintenance of cellular protein location are differentially expressed in porcine epithelial oviductal cells during longterm in vitro cultivation

determined from the optical density at 260 nm, and the RNA purity was estimated using the 260/280 nm absorption ratio (higher than 1.8) (NanoDrop spectrophotometer, Thermo Scientific, ALAB, Poland). The RNA integrity and quality were checked on a Bioanalyzer 2100 (Agilent Technologies, Inc., Santa Clara, CA, USA). The resulting RNA integrity numbers (RINs) were between 8.5 and 10 with an average of 9.2 (Agilent Technologies, Inc., Santa Clara, CA, USA). The RNA in each sample was diluted to a concentration of 100 ng/μl with an OD260/OD280 ratio of 1.8/2.0. From each RNA

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MR-proADM and MR-proANP levels in patients with acute pulmonary embolism

Diagnostics Inc., Tarrytown, USA). Serum BNP and TnI concentrations were performed on the ADVIA Centaur XP immunoassay analyzer (Siemens Healthcare Diagnostics Inc., Tarrytown, NY). Plasma D-dimer levels were assessed by using the BCS XP coagulation analyzer (Siemens Healthcare Diagnostics, Marburg, Germany). Serum MR-proADM and MR-proANP levels were measured with a commercially available kit using an ELISA, Human MR-proADM ELISA kit (Catalogue No: 201-12-7275 Sunred Biological Technology Co. Ltd, Shanghai) with a low sensitivity limit of 2.839 pg/mL. The samples were

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