Natalia Mazur-Panasiuk, Jacek Żmudzki and Grzegorz Woźniakowski
uptake of flies fed ASFV-infected blood.
ASFV resistance and stability have attracted the interest of numerous investigators over the years ( 5 , 7 , 10 , 23 , 26 , 27 , 28 , 41 , 42 , 47 ). It has been proved that ASFV shows high resistance to environmental conditions and remains infectious over a long storage time either below 0°C or at 4°C. The curing process of infected meat (a process like that which Parma, Iberian, or Serrano ham undergoes) facilitated survival of ASFV in ham for over a year ( 28 ). ASFV can survive many freeze–thaw cycles, and
binding inhibitors in the blood circulation of patients with adult-onset still disease. Arthritis Rheuma. 2001; 44:550-60.
20. Srichaikul T, Punyagupta S, Mongkolsritrakul W, Peutpol S, Leechawengwongs M, Chanokovat C. Hemophagocytic syndrome with fulminant multi-organ failure after tasting raw monkfish liver: the patient survived by immunomodulators and plasma exchange therapy. J Hematol Transfus Med. 2011; 21:261-6.
21. Srichaikul T, Mongkolsritrakul W. Hemophagocytic Syndrome in Textbook of Hematology-Diagnosis and Treatment of Common
Raluca M. Tat, Adela Golea, Ştefan C. Vesa and Daniela Ionescu
Despite fifty years of research, cardiac arrest (CA) and complications resulting from hypoxic organ injury, remains one of the most significant challenges faced by physicians, knowing that the vast majority of patients with CA have an unfavourable prognosis with a higher chance of death and severe neurological disabilities [ 1 , 2 , 3 ]. In the last few years, efforts have been focused on the best way to detect those patients who have a chance to survive and to recover.
In an attempt to identify patients who survived after resuscitated CA
Jytte Banner, Christian Bjerre Høyer, Martin Roest Christensen, Alexandra Gheorghe, Anne Bugge, Gyda Lolk Ottesen, Lene Warner Thorup Boel, Jørgen Lange Thomsen, Line Kruckow and Christina Jacobsen
Background: Forensic autopsy strategies may improve differential diagnostics both post-mortem and ante-mortem and aid in clinical settings concerning preventive efforts for premature mortality. Excess mortality and reduced life expectancy affect persons with severe mental illnesses (SMI) for multi-faceted reasons that remain controversial. Somatic conditions, medical treatment and lifestyle diseases, which are primarily examined in the living, contribute to premature deaths. The underlying pathophysiological mechanisms are unclear, though, and the benefits of a focused, standardised autopsy remain unproven. We have developed and implemented an optimised molecular–biological autopsy for deceased persons with SMI. Our aim is to map the occurrence of 1) somatic diseases and organ changes; 2) metabolic syndrome; 3) use and abuse of alcohol, pharmaceuticals and psychoactive substances; 4) pharmacokinetic and pharmacodynamic factors in the metabolism of pharmaceuticals; and 5) genetic variations (acquired and/or congenital) in sudden cardiac death. Additionally, we hope to contribute to diagnostic treatments and preventive measures to benefit those living with SMI. Methods: SURVIVE: let the dead help the living is a prospective, autopsy-based study on 500 deceased persons with SMI subjected to forensic autopsies under the Danish Act on Forensic Inquests and Autopsy. The autopsies followed an extended, standardised autopsy protocol comprised of whole-body computed tomography scanning, magnetic resonance imaging of the heart and brain and an extended forensic autopsy, including a wide panel of analyses (toxicology, microbiology, genetics, histology and biochemical analysis). Additionally, post-mortem data were linked to ante-mortem health data extracted from Danish national health registers.
Discussion: The SURVIVE autopsy procedure, including tissue sampling and bio banking, has been shown to be effective. We expect that the SURVIVE study will provide unique opportunities to unravel the mechanisms and causes of premature death in persons with SMI. We also expect that identifying prognostic biomarkers for comorbidities will contribute to prevention of premature deaths and comorbidities in persons with SMI.
Barbara Gajda, Barbara Szczęśniak-Fabiańczyk, Izabela Mandryk, Katarzyna Poniedziałek-Kempny, Florian Ryszka, Barbara Dolińska, Lucyna Leszczyńska and Zdzisław Smorąg
Prolactin (PRL) is a protein hormone synthesized in and secreted predominantly by lactotroph cells of the anterior pituitary gland. This hormone has been found to stimulate the immune system in animals. Because prolactin secreted in milk by the mother’s body is often insufficient, the administration of exogenous prolactin may significantly contribute to improving the health and growth of piglets. The aim of the study was to determine the dose of prolactin administered to newborn piglets to improve their survivability and growth rate. The study used Biolactin solution, produced on a semi-technical scale by FZNP Biochefa, which was administered per os to newborn piglets at a dose of 0.1, 0.5 and 1.0 mg PRL/kg body weight. NaCl physiological saline was used as a control. The number of piglets born alive, piglet body weight and mortality from birth to 21 and 28 days of age (weaning) were monitored. The study involved 98 litters (1197 piglets), which were divided into 3 experimental and 3 control groups. The experiment showed a positive effect of exogenous prolactin administered to newborn piglets on reducing their mortality and on increasing their birth to weaning growth rate. A dose of 0.5 mg PRL/kg body weight turned out to be optimal.
A. Bădilă, R. Manolescu, I. Japie, E. Bădilă, A. Papuc, C. Popovici, M. Tihulcă, A. Bujdei, D. Rădulescu, C. Cîrstoiu and R. Rădulescu
Aim: To assess the clinical results after osteosynthesis with locked intramedullary nail in metastasis of the long bones. Material and methods. We designed a prospective study in which we included all the patients with metastasis of the long bones admitted and surgically treated in our department between 2013 and 2015. Data for 64 were available at the final check-up. Our cohort totalized a number of 69 fractures (2 long bones required surgical treatment in 5 patients). The mean follow-up for survivors was 37 months (limits: 18-49 months). The primary tumor was known in 51 patients (79,69%). For the remaining 13 cases (20,31%), the primary tumor was not known and the pathological fracture was the first sign of the malignant disease. In the last group, the tumor could be identified by imagistic methods in 6 cases, while in other 3 cases, a biopsy and histological examination (which were performed in all the remaining 7 cases) determined the source organ. Clinical and radiological check-ups were performed at every 3 months in the first year and at every 6 months after that. Results. Pain amelioration and mobilization of the involved limb were achieved in all the cases. In 3 patients, the osteosynthesis could not compensate the progressive bone loss and the permanent use of an external orthosis was mandatory. The survival rate was 82,81% at 6 months and 67,19% at 12 months. Conclusions. All patients could be mobilized. Two thirds of the patients will survive more than a year. The goals of osteosynthesis are the same, regardless the location of the fracture and implant used: pain amelioration, stability for immediate full weight bearing, durability for patient’s life expectancy.
Vesna Todorovic, Gregor Sersa, Vid Mlakar, Damjan Glavac and Maja Cemazar
Assessment of the tumourigenic and metastatic properties of SK-MEL28 melanoma cells surviving electrochemotherapy with bleomycin
Background. Electrochemotherapy is a local treatment combining chemotherapy and electroporation and is highly effective treatment approach for subcutaneous tumours of various histologies. Contrary to surgery and radiation, the effect of electrochemotherapy on metastatic potential of tumour cells has not been extensively studied. The aim of the study was to evaluate the effect of electrochemotherapy with bleomycin on the metastatic potential of human melanoma cells in vitro.
Materials and methods. Viable cells 48 hours after electrochemotherapy were tested for their ability to migrate and invade through Matrigel coated porous membrane. In addition, microarray analysis and quantitative Real-Time PCR were used to detect changes in gene expression after electrochemotherapy.
Results. Cell migration and invasion were not changed in melanoma cells surviving electrochemotherapy. Interestingly, only a low number of tumourigenesis related genes was differentially expressed after electrochemotherapy.
Conclusions. Our data suggest that metastatic potential of human melanoma cells is not affected by electrochemotherapy with bleomycin, confirming safe role of electrochemotherapy in the clinics.
Joanna Zeyland, Daniel Lipiński and Ryszard Słomski
Mechanisms regulating the activity of the complement system responsible for the rejection of transplant organs are balanced so that the attack is instantaneous but is restricted to the infected cells of the organism. The most important components regulating its activity comprise CD55 and CD46 factors as well as the CD59 anchored in the cell membrane. Hyperacute response of the immunological system appears to be the key in the xenotransplant rejection and the elaboration of methods preventing its occurrence will give a real chance for the development of xenotransplantation.
Gene constructs containing coding sequences of human CD46, CD55 and CD59 were prepared and used to transfect porcine fetal fibroblasts. Stable lines were molecularly characterized for an integration of transgenes by PCR. Lines with a stable integration of transgenes were subjected to further characterization of expression by RT-PCR and vitality test. Molecular characteristics of the transgenic cell lines obtained revealed a steadfast integration and, in the majority of cases, expression of the introduced transgenes. The performed cytotoxicity analysis demonstrated that transgenic lines were characterised by a higher survivability rate than non-transgenic cells in the presence of human serum which proved their protective influence in relation to the activity of the complement system.
Jasna Petrovic, Jasna Jevdjic and Vladimir Jakovljevic
Care Med. 2000; 26(7): 883-92.
15. Joo K, Park W, Lim MJ, Kwon SR, Yoon J. Serum procalcitonin for differentiating bacterial infection from disease flares in patients with autoimmune diseases. J Korean Med Sci. 2011; 26(9): 1147-51.
16. Dellinger RP, Levy MM, Rhodes A, Annane D, Gerlach H, Opal SM, et al. Surviving sepsis campaign: international guidelines for management of severe sepsis and septic shock: 2012. Crit Care Med. 2013; 41(2): 580-637.
17. Sallah JI, Japiassu AM, Soares M, Assis EF, Gomes RN, Bozza MT, et al. Cytokine profiles as markers
Katarzyna Zych-Krekora, Michał Krekora, Marek Kopala and Maria Respondek-Libserska
Introduction: APVS (Absent Pulmonary Valve Syndrome) is a rare congenital heart disease. Its incidence according to The Polish National Registry of Fetal Cardiac Pathology in years 2004 - 2016 was 0.6%.This disease is caused by the absence or the residual pulmonary artery valve resulting in significant dilation of the pulmonary trunk and its branches. In utero deaths are reported. After birth the major problem is respiratory failure and high preoperative and postoperative mortality. Material and methods: In 1995 to 2016, 11 fetuses with APVS were diagnosed in our unit, at the average 27,5 weeks of gestation (min. 18.5- max 37.1 weeks of gestation). Two groups were analysed in this series of cases: “Old one” by 2011 (n = 6) and “New one” since 2011 (n = 5) and perinatal care as well as survival were compared. We analysed the fetal echo results, perinatal care including transplacental digoxin and steroids treatment in NEW group, the longevity of the pregnancy and neonatal/infants outcome. Results: In Old group the average day of cardiac surgery was day 91st after birth (max. 161) and the survival was 50% . In the New group the average surgery day was 41st day and the postoperative survival was 60%, however there was no statistical significance ( p > 0,05). Conclusions: There is no single parameter from prenatal life in foetuses with APVS which may allow to predict the positive outcome meaning neonatal survival. However optimal perinatal care (early detection of defect, transplacental digoxin at least 3-4 weeks, steroids, no preterm delivery, on-time delivery, postnatal care in tertiary center) and relatively early cardiac surgery may have combined impact on the improvement of survival after prenatal diagnosis of APVS, however more data are necessary to prove this hypothesis.