The aim of this study was to assess the condition of soil after serpentine fertilisation by determining the activity of phosphatase and some physicochemical properties of soil. The study was conducted in southern Poland in the Wisła Forest District (49°8’12.92N 18°58’56.36E). The following variants: C - control - no fertiliser; S - ground serpentinite; SN - serpentinite + nitrogen; SP - ground serpentinite + P; SNP - ground serpentinite + NP; SNPK - ground serpentinite + NP (as above) + K. Fertilisation of serpentinite stimulates the activity of phosphatase in soil under spruce stands. The positive impact is reflected in the organic horizon. Less activity was noted in the humus-mineral horizon (AE) in all the fertiliser combinations three years after the fertilisation. Fertilisation of serpentinite improves the chemical properties - pH was increased, reduction of molar ratio of exchangeable calcium to magnesium form was noted.