://www.uniprot.org [ 30 , 31 ]. Protein primary sequences were decomposed into overlapping pentapeptides offset by one residue, ie, MGVPF, GVPFF, and VPFFS. Next, each pentapeptide was analyzed for occurrences in viral proteomes using Peptide Match program ( https://research.bioinformatics.udel.edu/peptidematch ) [ 32 ].
Proteomes from nine viruses were analyzed: influenza A virus, H1N1 (NCBI:txid211044), influenza A virus, H3N2 (NCBI:txid385580), influenza A virus, H5N1 (NCBI:txid93838), influenza A virus, H10N7 (NCBI:txid382838), influenza B virus (NCBI:txid518987), and influenza
peptide commonality between influenza hemagglutinin (HA) and the human proteome [ 27 , 28 , 29 ]. Such a peptide sharing might be potentially involved in neuropsychiatric disorders [ 28 , 29 ]. Instead, to the best of the current knowledge, no data have been reported on cross-reactivity as a possible autoimmune link between influenza infection and SUD. Using titin as a protein that, when altered, may associate with sudden death [ 30 , 31 , 32 , 33 , 34 ], this study specifically explored the peptide commonality between influenza viruses and titin, found viral vs
Aleksandra Orzołek, Paweł Wysocki, Jerzy Strzeżek, Magdalena Koziorowska-Gilun, Anna Dziekońska and Władysław Kordan
The aim of the study was to analyse sperm proteomes of ejaculates from Polish Large White (PLW) and Polish Landrace (PL) boars and to identify differences which putatively influence semen quality. Spermatozoa protein profiles were analysed by electrophoretic methods followed by selected techniques to evaluate semen quality on the following factors: sperm motility, lipid peroxidation levels (MDA production), ATP content, activities of superoxide dismutase (SOD) and catalase (CAT), total antioxidant status (TAS), and total oxidant status (TOS) of seminal plasma. A protein with an estimated molecular weight of 30 kDa was found in spermatozoa of selected ejaculates. Mass spectrometry demonstrated that this polypeptide is most similar to proacrosin binding protein (sp32). The presence of the protein was more frequently observed in sperm extracts obtained in spring-summer period. Ejaculates containing sp32-like protein demonstrated significantly higher spermatozoa motility, lower inhibition of MDA production by seminal plasma, and higher SOD activity in seminal plasma. Boar semen which included sp32-like protein also demonstrated lower ATP levels in spermatozoa as well as higher TAS and lower TOS of seminal plasma, though the differences were not statistically significant. Ejaculates from PLW boars, with sp32-like protein present in sperm, were characterised by significantly higher sperm motility, lower ATP content in spermatozoa, and higher TAS of seminal plasma. The diminished parameters of semen quality were observed in ejaculates from PL boars that also contained the discussed protein, but the differences were not statistically significant. These findings suggest that the presence of sp32-like protein in boar spermatozoa could influence semen quality
Francisc Andrei Boda, Anca Mare, Zoltán István Szabó, Lavinia Berta, Augustin Curticapean, Maria Dogaru and Adrian Man
) venoms: Taxonomic and toxino-logical implications. Rapid Commun Mass Spectrom. 2002;16:600–8. DOI: 10.1002/rcm.613
33. Petras D, Heiss P, Harrison RA, Süssmuth RD, Calvete JJ. Top-down venomics of the East African green mamba, Dendroaspis angusticeps, and the black mamba, Dendroaspis polylepis, highlight the complexity of their toxin arsenals. J Proteomics. 2016;146:148–64. DOI: 10.1016/j.jprot.2016.06.018
34. Huang HW, Liu BS, Chien KY, Chiang LC, Huang SY, Sung WC, et al. Cobra venom proteome and glycome determined from individual snakes of Naja atra
J. Šimková, M. Milkovičová, M. Valko-Rokytovská, Z. Kostecká, E. Bencúrová, L. Pulzová, Ľ. Čomor and M. R. Bhide
and metabolism, Haptoglobin. [online], http://eggnogdb.embl.de/#/app/results#ENOG410CHIEdatamenu .
12. Gao, Y., Chen, C., Zhao, J., Zhang, P., Chai, Z., He, W., Huang, Y., 2003: An improved method for analysis of distribution of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence. Anal. Chim. Acta , 485, 131—137.
13. Hagedoorn, P. L., 2015: Microbial Metalloproteomics. Proteomes , 3, 424.
14. Hara, M., Fujinaga, M., Kuboi, T., 2005: Metal binding by citrus dehydrin with histidine-rich domains. J. Exp. Bot. , 56
Kerem Teke, Hasan Yilmaz, Mehmet Esad Kosem, Mustafa Yuksekkaya, Sema Yusufoglu, Busra Yaprak Bayrak, Yusufhan Yazir, Demir Kursat Yildiz and Ozdal Dillioglugil
Bacillus Calmette-Guerin at the proteome level in an in vivo rat model. Cancer Immunol Immunother. 2018;67(1):67-77. DOI: 10.1007/s00262-017-2063-2
6. Teke K, Ozkan TA, Cebeci OO, Yilmaz H, Keles ME, Ozkan L, et al. Preventive effect of intravesical ozone supplementation on n-methyl-n-nitrosourea-induced non-muscle invasive bladder cancer in male rats. Exp Anim. 2017;66(3):191-8. DOI: 10.1538/expanim.16-0093
7. Reis LO, Favaro WJ, Ferreira U, Billis A, Fazuoli MG, Cagnon VH. Evolution on experimental animal model for upper urothelium carcinogenesis. World J
Bogdan Dębski, Tadeusz Nowicki, Wojciech Zalewski, Agnieszka Bartoszewicz and Jan Twardoń
Proteome Res 2011, 10, 4252–4262.
16. Kuryl T., Adamowicz M., Dębski B., Bertrandt J., Martynik K.: Degradation of (9, 10)- 3 H-myristic acid by lymphocytes. Screening test of inherited disorders of activation, transport, and mitochondrial oxidation of fatty acids. Ateroskleroza 2001, 5, 23–27.
17. Lee D.N., Yen H.T., Shen T.F., Chen B.J.: Chromium-induced glucose uptake, superoxide anion production and phagocytosis in cultured pulmonary alveolar macrophages of weaning pigs. Biol Trace Elem Res 2000, 77, 53–64.
18. Manning N.J., Olpin S.E., Pollitt R
Barbara Stawiarska-Pięta, Jolanta Zalejska-Fiolka, Magdalena Wyszyńska, Anna Kleczka, Beata Janiga, Natalia Grzegorzak and Ewa Birkner
.H., Korczak J., Małecka M., Mildner – Szkudlarz S., Rudzińska M., Samotyja U., Zawirska-Wojtasiak R.: Oxidation of lipids in food. Pol J Food Nutr Sci 2004, 13/54, 87–100.
27. Wisniewski J.R., Friedrich A., Keller T., Mann M., Koepsell H.: The impact of high-fat diet on metabolism and immune defense in small intestine mucosa. J Proteome Res 2015, 14, 353−365.
28. Xu J., Zhou X., Gao H., Chang Ch., Deng Q., Huang Q., Ma J., Wan Z., Yang J., Huang F.: Micronutrients-fortified rapeseed oil improves hepatic lipid accumulation and oxidative stress in rats fed a high
.A., Kiselev O.I.: Molecular mechanisms enhancing the proteome of influenza A viruses: an overview of recently discovered proteins. Virus Res 2014, 185, 53–63.
29. Wille M., Tolf C., Avril A., Latorre-Margalef N., Wallerström S., Olsen B., Waldenström J.: Frequency and patterns of reassortment in natural influenza A virus infection in a reservoir host. Virology 2013, 443, 150–160.