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Pollen Transcriptome and Proteome: Molecular and Functional Analysis

] HEIDSTRA R, WELCH D, SCHERES B. Mosaic analyses using marked activation and deletion clones dissect Arabidopsis SCARECROW action in asymmetric cell division. Genes Dev 2004; 18: 1964-1969 [33] HEPLER PK, VIDALI L, CHEUNG AY. Polarized cell growth in higher plants. Annu Rev Cell Dev Biol 2001; 17: 159-187. [34] HOLMES-DAVIS R, TANAKA CK, VENSEL WH, HURKMAN WJ, McCORMICK S. Proteome mapping of mature pollen of Arabidopsis thaliana. Proteomics 2005; 5: 4864-4884. [35] HONYS D, COMBE JP, TWELL D, ČAPKOVÁ V. The translationally

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Urinary Proteome Analysis using Capillary Electrophoresis Coupled to Mass Spectrometry: A Powerful Tool in Clinical Diagnosis, Prognosis and Therapy Evaluation

standards. Proteomics Clin Appl 2007; 1: 148-56. Coon JJ, Zürbig P, Dakna M, Dominiczak AF, Decramer S, Fliser D, et al. CE-MS analysis of the human urinary proteome for biomarker discovery and disease diagnostics. Proteomics Clin Appl 2008; 2: 964-73. Castronovo V, Kischel P, Guillonneau F, De LL, Defechereux T, De PE, et al. Identification of specific reachable molecular targets in human breast cancer using a versatile ex vivo proteomic method. Proteomics 2007; 7: 1188-96. Roessler M

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Application of Preparative Electrophoresis for Clinical Proteomics in Urine: Is it Feasible?

References Thongboonkerd V. Recent progress in urinary proteomics. Proteomics Clin Appl 2007; 1: 780-91. Fliser D, Novak J, Thongboonkerd V, Argilés A, Jankowski V, Girolami MA, Jankowski J, Mischak H. Advances in urinary proteome analysis and biomarker discovery. J Am Soc Nephrol 2007; 18 (4): 1057-71. Decramer S, Gonzalez de Peredo A, Breuil B, Mischak H, Monsarrat B, Bascands JL, Schanstra JP. Urine in clinical proteomics. Mol Cell Proteomics 2008; 7 (10): 1850

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Glycans as Biomarkers: Status and Perspectives

References Apweiler R, Hermjakob H, Sharon N. On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database. Biochem Biophys Acta 1999; 1473: 4-8. Hortin GL, Sviridov D, Anderson NL. High abundance polypeptides of the human plasma proteome comprising the top 4 logs of polypeptide abundance. Clin Chem 2008; 54: 1608-16. Sharon N, Lis H. Glycoproteins: Structure and Function. In: Gabius HJ, Gabius S, editors. Glycoscience: Status and Perspectives

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Changes in the proteomic profile of blood serum in coronary atherosclerosis

Summary

Background

Our aim was to study changes in the serum proteomic profile in coronary atherosclerosis.

Methods

The study involved two groups of patients: 1) men with coronary heart disease and coronary atherosclerosis (n = 15); 2) control (n = 15): men without coronary heart disease. The object of this study was blood serum. Separation of proteins for the investigation of differences in serum protein components was performed by two-dimensional electrophoresis. Identification of protein fractions was carried out using peptide mass maps by the matrix-assisted laser desorption ionization method.

Results

In blood serum samples from patients with coronary atherosclerosis, protein separation in two-dimensional gels with mass-spectrometric identification revealed an increase of some proteins: hemopexin, transthyretin (monomeric form), retinol-binding protein 4, and components of the complement system: C3 (chain B) and C9. There was a decrease of some proteins: kininogen, zinc finger protein 133, and B-cell CLL/lymphoma 6 member B protein. Comparisons between the experimental and control group were carried out in protein fractions where the protein amount differed more than 1.5-fold (p < 0.05).

Conclusions

Proteome profiling of serum revealed a change in the content of kininogen, hemopexin, transthyretin, retinol-binding protein, and proteins of the complement system (C9, and C3) in coronary atherosclerosis. The contribution to the differential expression of a protein was often made by isoforms of the protein, particularly transthyretin. The change in the concentrations of functionally interacting proteins, such as transthyretin and retinol-binding protein, were noted.

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Proteins: from electrophoresis to proteomics 5th EFCC Symposium for Balkan Region

, Metzger J. Urinary proteome analysis using capillary electrophoresis coupled to mass spectrometry: a powerful tool in clinical diagnosis, prognosis and therapy evaluation. Journal of Med Biochem 2009; 28: 223-34. Sypniewska G, Sawick M, Krintus M, Kozinski M, Kubica J. The use of biochip cardiac array technology for early diagnosis of acute coronary syndromes. Journal of Med Biochem 2009; 28: 293-9.

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Evaluation of the Pattern of Human Serum Glycoproteins in Prostate Cancer

References Ullah MF, Aatif M. The footprints of cancer development: Cancer biomarkers. Cancer Treat Rev 2009; 35: 193-200. Taniguchi N, Hancock W, Lubman DM, Rudd PM. The second golden age of glycomics: from functional glycomics to clinical applications. J Proteome Res 2009; 8: 425-6. Brockhausen I. Kuns W, eds. Glycoproteins and Human Disease. Hidelberg: Medical Intelligence Unit, Springer; 1997. Dennis JW, Granovsky M, Warren CE. Glycoprotein

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From Research to Clinical Application Multi-Parameter Testing: Marker Panels for the Early Detection of Complex Diseases

References Schmidt A, Kellermann J, Lottspeich F. A novel strategy for quantitative proteomics using isotope-coded protein labels. Proteomics 2005; 5: 4-15. Haab BB, Geierstanger BH, Michailidis G, Vitzthum F, Forrester S, Okon R, Saviranta P, Brinker A, Sorette M, Perlee L, Suresh S, Drwal G, Adkins JN, Omenn GS. Immunoassay and antibody microarray analysis of the HUPO Plasma Proteome Project reference specimens: systematic variation between sample types and calibration of mass spectrometry data

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Role of Redox Metals, Oxidative Protein Products and Antioxidant Potentials of Thiols in Diabetic Retinopathy

; 30(2): 195-202. Qiuju L, Liang S, Yi T, Guanjun W, Xu L, Lu C. Role of iron deficiency and overload in the pathogenesis of diabetes and diabetic complications. Current Medicinal Chemistry 2009; 16(1): 113-29. Gao BB, Chen X, Timothy N, Aiello LP, Feener EP. Characterization of the vitreous proteome in diabetes without diabetic retinopathy and diabetes with proliferative diabetic retinopathy. Journal of Proteome Research 2008; 7(6): 2516-25. Yamagishi SI, Ueda S, Matsui T, Nakamura K

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Human Serum Low Molecular Mass Prostate-Specific Antigen as Biomarker

-specific antigen and prostatic acid phosphatase in prostate cancer and benign disease seminal plasma fluids. J Proteome Res 2009; 8: 620-30. 10. Pampalakis G, Scorilas A, Sotiropoulou G. Novel splice variants of prostate-specific antigen and applications in diagnosis of prostate cancer. Clin Biochem 2008; 41: 591-7. 11. UniProt. Available at: http://www.uniprot.org/uniprot/P07288. Accessed: 26 Jan 2017. 12. Dwek MV, Jenks A, Leathem AJ. A sensitive assay to measure biomarker glycosylation demonstrates increased fucosylation of

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