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Review Article. Technical aspects of oxygen level regulation in primary cell cultures: A review

Abstract

Oxygen (O2) is an essential element for aerobic respiration. Atmospheric concentration of O2 is approximately 21%. Mammalian cells, however, are generally adapted to O2 levels much lower than atmospheric conditions. The pericellular levels of O2 must also be maintained within a fairly narrow range to meet the demands of cells. This applies equally to cells in vivo and cells in primary cultures. There has been growing interest in the performance of cell culture experiments under various O2 levels to study molecular and cellular responses. To this end, a range of technologies (e.g. gas-permeable technology) and instruments (e.g. gas-tight boxes and gas-controlled incubators) have been developed. It should be noted, however, that some of these have limitations and they are still undergoing refinement. Nevertheless, better results should be possible when technical concerns are taken into account. This paper aims to review various aspects of O2 level adjustment in primary cell cultures, regulation of pericellular O2 gradients and possible effects of the cell culture medium.

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Original Article. Toxicity of Glandularia selloi (Spreng.) Tronc. leave extract by MTT and neutral red assays: influence of the test medium procedure

: Investigation of involvement of L-arginina-nitric oxide-cyclic guanoside monophosphate pathway. J Ethnopharmacol, 37, 864-74. Zhang SZ, Lipsky MM, Trump BF, Hsu IC. (1990). Neutral red (NR) assay for cell viability and xenobiotic-induced cytotoxicity in primary cultures of human and rat hepatocytes. Cell Biology and Toxicology, 6, 219-34. Zucco F, De Angelis I, Testai E, Stammati A. (2004). Toxicology investigations with cell culture systems: 20 years after. Toxicol In Vitro, 18, 153-63.

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Advances in acute toxicity testing: strengths, weaknesses and regulatory acceptance

REFERENCES Ambuja SB, Elaina K, Thomas JF, John CL, Donna LM, Thomas H, Geoffrey WP. (2013). Correlating in vitro data to in vivo findings for risk assessment. Altex 31 : 1/14. Symposium report. Arwa BR, Vladimir BB. (2016). In silico toxicology: computational methods for the prediction of chemical toxicity. Wiley Interdiscip Rev Comput Mol Sci . 6 : 147–172. Blais A, Morvan-Baleynaud J, Friedlander G, Le Grimellec C. (1993). Primary culture of rabbit proximal tubules as a cellular model to study nephrotoxicity of xenobiotics. Kidney

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Chronic alcoholism-mediated metabolic disorders in albino rat testes

, Staudinger JL, Watson MA, Jones SA, McKee DD, Oliver BB, Willson TM, Zetterstrom RH, Perlmann T, Lehmann JM. (1998). An orphan nuclear receptor activated by pregnanes defi nes a novel steroid signaling pathway. Cell 92: 73-82. Kostrubsky VE, Strom SC, Wood SG, Wrighton SA, Sinclair PR, Sinclair JF. (1995) Ethanol and isopentanol increase CYP3A and CYP2E in primary cultures of human hepatocytes. Arch Biochem Biophys 322: 516-520. Kovalenko VM, Bagnyukova TV, Sergienko OV, Bondarenko LB, Shayakhmetova GM, Matvienko AV, Pogribny IP. (2007

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