Resnicoff M, Rubini M, Baserga R, Rubin R. Ethanol inhibits insulin-like growth factor-1-mediated signalling and proliferation of C6 rat glioblastoma cells. Lab Invest 1994;71:657-62.
Luo J, Miller MW., Growth factor-mediated neural proliferation: target of ethanol toxicity. Brain Res Brain Res Rev 1998;27:157-67.
Guerri C, Sáez R, Sancho-Tello M, Martin de Aquilera E, Renau-Piqueras J. Ethanol alters astrocyte development: a study of critical periods using primarycultures. Neurochem Res 1990
The Effect of Laurel Leaf Extract Against Toxicity Induced by 2,3,7,8-Tetrachlorodibenzo-P-Dioxin in Cultured Rat Hepatocytes
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a very toxic environmental pollutant that raises great public concern about its impact on human health. Recent studies indicate that laurel leaf extract exhibits antioxidant properties that can counter the toxic effects of certain compounds in the liver. The aim of this study was to assess how effective LE is against the toxicity of TCDD in a primary culture of rat hepatocytes. The extract (50 mg L-1, 100 mg L-1, and 200 mg L-1) was added to cultures alone or with TCDD (1.61 mg L-1 and 3.22 mg L-1) for 48 hours. Cell viability was measured using the [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) assay and the lactate dehydrogenase (LDH) cytotoxicity assay, while oxidative damage was assessed by measuring total antioxidant capacity (TAC) and total oxidative stress (TOS). DNA damage was also analysed using the micronucleus (MN) assay of the cultured hepatocytes. TCDD alone lowered, and laurel extract had no effect on cell viability. TCDD also increased TOS and significantly decreased TAC. It significantly increased the frequency of micronucleated hepatocytes in a dose-dependent manner. In cultures exposed to LE alone, TOS did not change and TAC significantly increased in a dose-dependent manner. Added to TCDD, laurel countered its toxic effects and showed protective effects against TCDD-mediated DNA damage. This points to the therapeutic potential of laurel against TCDD toxicity in the liver.
myelin formation in aggregating brain cell culture. Neurotoxicology 1999;20:41-8.
29. Kwon OS, Slikker Jr. W, Davies DL. Biochemical and morphological effects of fumonisin B1 on primarycultures of rat cerebrum. Neurotoxicol Teratol 2000;22:565-72.
30. Galvano F, Campisi A, Russo A, Galvano G, Palumbo M, Renis M, Barcellona ML, Perez-Polo JR, Vanella A. DNA damage in astrocytes exposed to fumonisin B1. Neurochem Res 2002;27:345-51.
31. Mobio TA, Anane R, Baudrimont I, Carratu M-R, Shier TW, Dano SD, Ueno Y, Creppy EE
mouse. Carcinogenesis 2006;27:1803-11.
Kellis JT Jr, Vickery LE. Inhibition of human estrogen synthetase (aromatase) by flavones. Science 1984;225:1032-4.
Oršolić N, Štajcar D, Bašić I. Cytotoxicity of propolis and its polyphenolic compounds on primaryculture of human urinary bladder transitional cell carcinoma. Pharmacologyonline 2006;3: 408-15.
Edwards DJ, Bernier SM. Inhibitory effect of grapefruit juice and its bitter principal, naringenin, on CYP1A2 dependent metabolism of
Mohammad Ali Eghbal, Narges Abdoli and Yadollah Azarmi
-galactosamine in primaryculture of human hepatocytes. Chem Biol Interact 2009;181:95-106. doi: 10.1016/j.cbi.2009.06.003
16. Moldéus P, Thor H, Högberg J, Orrenius S. Drug metabolism and toxicity studies in isolated rat liver cells. International congress series no. 417. Amsterdam: Excerpta medica; New York: distributed by Elsevier North-Holland; 1977. p. 75-84.
17. Heidari R, Babaei H, Eghbal MA. Cytoprotective effects of taurine against toxicity induced by isoniazid and hydrazine in isolated rat hepatocytes. Arh Hig Rada Toksikol 2013
Malay Chaklader, Prosun Das, Jacintha Archana Pereira, Samaresh Chaudhuri and Sujata Law
16. Kim J, Tang JY, Gong R, Kim J, Lee JJ, Clemons KV, Chong CR, Chang KS, Fereshteh M, Gardner D, Reya T, Liu JO, Epstein EH, David A. Stevens DA, Beachy PA. Itraconazole, a commonly used antifungal that inhibits hedgehog pathway activity and cancer growth. Cancer Cell 2010;17:388-99.
17. Laryea D, Gullbo J, Isaksoon A, Larsson R, Nygren P. Characterization of the cytotoxic properties of the benzimidazole fungicide, benomyl and carbendazim, in human tumor cell lines and primaryculture of patient tumor cells. Anticancer Drugs 2010
Ana Marija Marjanović, Ivan Pavičić and Ivančica Trošić
/Plenum Publishers; 1999. p. 967-70.
66. Adey WR. Cell and molecular biology associated with radiation fi elds of mobile telephones. In: Stone WR, Ueno S, editors. Review of radio science 1996-1999. Oxford: Oxford University Press; 1999. p. 845-72.
67. Campisi A, Gulino M, Acquaviva R, Bellia P, Raciti G, Grasso R, Musumeci F, Vanella A, Triglia A. Reactive oxygen species levels and DNA fragmentation on astrocytes in primaryculture after acute exposure to low intensity microwave electromagnetic fi eld. Neurosci Lett 2010
Jean-Paul Bourdineaud, Maja Šrut, Anamaria Štambuk, Mirta Tkalec, Daniel Brèthes, Krešimir Malarić and Göran I .V. Klobučar
species levels and DNA fragmentation on astrocytes in primaryculture after acute exposure to low intensity microwave electromagnetic field. Neurosci Lett 2010;473:52-5. doi: 10.1016/j.neulet.2010.02.018
37. Roux D, Vian A, Girard S, Bonnet P, Paladian F, Davies E, Ledoigt G. Electromagnetic fields (900 MHz) evoke consistent molecular responses in tomato plants. Physiol P l a n t a r u m 2 0 0 6 ; 1 2 8 : 2 8 3 - 8 . d o i : 10.1111/j.1399-3054.2006.00740.x
38. Roux D, Vian A, Girard S, Bonnet P, Paladian F, Davies E, Ledoigt G. High
potential by primarycultures of ocular tissues: I. Characterization of rabbit corneal epithelial cells and initial injury and delayed toxicity studies. Toxicology 1992;76:153-76.
Bodor N, Kaminski JJ, Selk S. Soft drugs. 1. Labile quaternary ammonium salts as soft antimicrobials. J Med Chem 1980;23:469-74.
Singh KS, Bajpai M, Tyagi VK. Amine oxides: A review. J Oleo Sci 2006;55:99-119.
Toney CJ, Fredli FE, Frank PJ. Kinetics and preparation of amine oxides. J Am Oil Chem Soc 1994
75. Moffat C, Buckland ST, Samson AJ, McArthur R, Chamosa Pino V, Bollan KA, Huang JT, Connolly CN. Neonicotinoids target distinct nicotinic acetylcholine receptors and neurons, leading to differential risks to bumblebees. Sci Rep 2016;6:24764. doi: 10.1038/srep24764
76. Stanley DA, Smith KE, Raine NE. Bumblebee learning and memory is impaired by chronic exposure to a neonicotinoid pesticide. Sci Rep 2015;5:16508. doi:10.1038/srep16508
77. Wilson DE, Velarde RA, Fahrbach SE, Mommaerts V, Smagghe G. Use of primarycultures of Kenyon cells from