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Comparison of Ethanol and Acetaldehyde Toxicity in Rat Astrocytes in Primary Culture

;16:233-40. Resnicoff M, Rubini M, Baserga R, Rubin R. Ethanol inhibits insulin-like growth factor-1-mediated signalling and proliferation of C6 rat glioblastoma cells. Lab Invest 1994;71:657-62. Luo J, Miller MW., Growth factor-mediated neural proliferation: target of ethanol toxicity. Brain Res Brain Res Rev 1998;27:157-67. Guerri C, Sáez R, Sancho-Tello M, Martin de Aquilera E, Renau-Piqueras J. Ethanol alters astrocyte development: a study of critical periods using primary cultures. Neurochem Res 1990

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Atipamezole Attenuated Telazol/Xylazine-Induced Expression of C-Fos in Rat Thalamencephal and Cerebral Cortex

: Association of c-fos mRNA expression and excitotoxicity in primary cultures of mouse neocortical and cerebellar neurons. Journal of Neuroscience Research 1997, 48(6): 533-542. 23. Willoughby JO, Mackenzie L, Medvedev A, Hiscock JJ: Fos induction following systemic kainic acid: early expression in hippocampus and later widespread expression correlated with seizure. Neurosience 1997, 77(2): 379-392. 24. Niles LP, Smith LJ, Tenn CC: Modulation of c-fos expression in the rat striatum by diazepam. Neuroscience Letters 1997, 236(1): 5

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Myofibroblasts in Normal and Fibrotic Liver in Different Animal Species

(8-9):1639-1642. 37. Burt AD: Pathobiology of hepatic stellate cells. J Gastroenterol 1999, 34(3):299-304. 38. Friedman SL, Rockey DC, McGuire RF, Maher JJ, Boyles JK, Yamasaki G: Isolated hepatic lipocytes and Kupffer cells from normal human liver: morphological and functional characteristics in primary culture. Hepatology 1992, 15(2):234-243. 39. Reeves HL, Friedman SL: Activation of hepatic stellate cells--a key issue in liver fibrosis. Front Biosci 2002, 7:d808-826. 40. Friedman SL: Molecular regulation of hepatic fibrosis, an

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Review Article. Technical aspects of oxygen level regulation in primary cell cultures: A review

Abstract

Oxygen (O2) is an essential element for aerobic respiration. Atmospheric concentration of O2 is approximately 21%. Mammalian cells, however, are generally adapted to O2 levels much lower than atmospheric conditions. The pericellular levels of O2 must also be maintained within a fairly narrow range to meet the demands of cells. This applies equally to cells in vivo and cells in primary cultures. There has been growing interest in the performance of cell culture experiments under various O2 levels to study molecular and cellular responses. To this end, a range of technologies (e.g. gas-permeable technology) and instruments (e.g. gas-tight boxes and gas-controlled incubators) have been developed. It should be noted, however, that some of these have limitations and they are still undergoing refinement. Nevertheless, better results should be possible when technical concerns are taken into account. This paper aims to review various aspects of O2 level adjustment in primary cell cultures, regulation of pericellular O2 gradients and possible effects of the cell culture medium.

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The Effect of Laurel Leaf Extract Against Toxicity Induced by 2,3,7,8-Tetrachlorodibenzo-P-Dioxin in Cultured Rat Hepatocytes

The Effect of Laurel Leaf Extract Against Toxicity Induced by 2,3,7,8-Tetrachlorodibenzo-P-Dioxin in Cultured Rat Hepatocytes

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a very toxic environmental pollutant that raises great public concern about its impact on human health. Recent studies indicate that laurel leaf extract exhibits antioxidant properties that can counter the toxic effects of certain compounds in the liver. The aim of this study was to assess how effective LE is against the toxicity of TCDD in a primary culture of rat hepatocytes. The extract (50 mg L-1, 100 mg L-1, and 200 mg L-1) was added to cultures alone or with TCDD (1.61 mg L-1 and 3.22 mg L-1) for 48 hours. Cell viability was measured using the [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) assay and the lactate dehydrogenase (LDH) cytotoxicity assay, while oxidative damage was assessed by measuring total antioxidant capacity (TAC) and total oxidative stress (TOS). DNA damage was also analysed using the micronucleus (MN) assay of the cultured hepatocytes. TCDD alone lowered, and laurel extract had no effect on cell viability. TCDD also increased TOS and significantly decreased TAC. It significantly increased the frequency of micronucleated hepatocytes in a dose-dependent manner. In cultures exposed to LE alone, TOS did not change and TAC significantly increased in a dose-dependent manner. Added to TCDD, laurel countered its toxic effects and showed protective effects against TCDD-mediated DNA damage. This points to the therapeutic potential of laurel against TCDD toxicity in the liver.

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Determining Influence of Culture Media and Dose-Dependent Supplementation with Basic Fibroblast Growth Factor on the Ex Vivo Proliferative Activity of Domestic Cat Dermal Fibroblasts in Terms of Their Suitability for Cell Banking and Somatic Cell Cloning of Felids

: 205–213. Kanazawa S., Fujiwara T., Matsuzaki S., Shingaki K., Taniguchi M., Miyata S., Tohyama M., Sakai Y., Yano K., Hosokawa K., Kubo T. (2010). bFGF regulates PI3-kinase-Rac1-JNK pathway and promotes fibroblast migration in wound healing. PLoS One, 5(8): e12228. Kim G.A., Oh H.J., Kim M.J., Jo Y.K., Choi J., Kim J.W., Lee T.H., Lee B.C. (2015). Effect of primary culture medium type for culture of canine fibroblasts on production of cloned dogs. Theriogenology, 84: 524–530. Lee H.S., Yu X.F., Bang J.I., Cho S.J., Deb G.K., Kim B.W., Kong I

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Fumonisin B1: A Neurotoxic Mycotoxin / Fumonizin B1: Neurotoksični Mikotoksin

myelin formation in aggregating brain cell culture. Neurotoxicology 1999;20:41-8. 29. Kwon OS, Slikker Jr. W, Davies DL. Biochemical and morphological effects of fumonisin B1 on primary cultures of rat cerebrum. Neurotoxicol Teratol 2000;22:565-72. 30. Galvano F, Campisi A, Russo A, Galvano G, Palumbo M, Renis M, Barcellona ML, Perez-Polo JR, Vanella A. DNA damage in astrocytes exposed to fumonisin B1. Neurochem Res 2002;27:345-51. 31. Mobio TA, Anane R, Baudrimont I, Carratu M-R, Shier TW, Dano SD, Ueno Y, Creppy EE

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Cytotoxicity of Polyphenolic/Flavonoid Compounds in a Leukaemia Cell Culture

mouse. Carcinogenesis 2006;27:1803-11. Kellis JT Jr, Vickery LE. Inhibition of human estrogen synthetase (aromatase) by flavones. Science 1984;225:1032-4. Oršolić N, Štajcar D, Bašić I. Cytotoxicity of propolis and its polyphenolic compounds on primary culture of human urinary bladder transitional cell carcinoma. Pharmacologyonline 2006;3: 408-15. Edwards DJ, Bernier SM. Inhibitory effect of grapefruit juice and its bitter principal, naringenin, on CYP1A2 dependent metabolism of

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Original Article. Toxicity of Glandularia selloi (Spreng.) Tronc. leave extract by MTT and neutral red assays: influence of the test medium procedure

: Investigation of involvement of L-arginina-nitric oxide-cyclic guanoside monophosphate pathway. J Ethnopharmacol, 37, 864-74. Zhang SZ, Lipsky MM, Trump BF, Hsu IC. (1990). Neutral red (NR) assay for cell viability and xenobiotic-induced cytotoxicity in primary cultures of human and rat hepatocytes. Cell Biology and Toxicology, 6, 219-34. Zucco F, De Angelis I, Testai E, Stammati A. (2004). Toxicology investigations with cell culture systems: 20 years after. Toxicol In Vitro, 18, 153-63.

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Efficiency of hepatocyte pretreatment with coenzyme Q10 against statin toxicity

-galactosamine in primary culture of human hepatocytes. Chem Biol Interact 2009;181:95-106. doi: 10.1016/j.cbi.2009.06.003 16. Moldéus P, Thor H, Högberg J, Orrenius S. Drug metabolism and toxicity studies in isolated rat liver cells. International congress series no. 417. Amsterdam: Excerpta medica; New York: distributed by Elsevier North-Holland; 1977. p. 75-84. 17. Heidari R, Babaei H, Eghbal MA. Cytoprotective effects of taurine against toxicity induced by isoniazid and hydrazine in isolated rat hepatocytes. Arh Hig Rada Toksikol 2013

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