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Beneficial effect of 6 weeks lasting handling of adult rats on spatial memory in experimental model of neurodegeneration

performance in rats. Behav Brain Res 176 : 267–273. Deutsch-Feldman M, Picetti R, Seip-Cammack K, Zhou Y, Kreek MJ. (2015). Effects of handling and vehicle injections on adrenocorticotropic and corticosterone concentrations in Sprague-Dawley compared with Lewis rats. J Am Assoc Lab Sci 54 : 35–39. Fenoglio KA, Brunson KL, Avishai-Eliner S, Stone BA, Kapadia BJ, Baram TZ. (2005). Enduring, handling-evoked enhancement of hippocampal memory function and glucocorticoid receptor expression involves activation of the corticotropin-releasing factor type 1 receptor

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Effects of mustard oil cake on liver proteins of Channa punctatus (Bloch)

REFERENCES Abbas S, Ahimed L, Akhter P, Asghar T. (2001). Response of urea on the growth performance of major carp viz., Labeo rohita, Catla catla and Chirinus mrigala. Pakistan Vet J 21 (4): 180–183. Adamu KM, Siakpere OK. (2011). Effect of sub lethal concentration of tobacco( Nicotina tobaccum) leaf dust on some biochemical parameters of Hybrid Catfish( Clarias gariepinus and Heterobranchus bidorsalis. Braz Arch Biol Technol 54 (1): 183–196. Ahmed DFI. (2013). Effect of industrial waste discharge including heavy metals Burullus lake

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Sub-chronic oral toxicity assessment (90 days) of ethanolic fraction of leaves of Neurocalyx calycinus (R. Br. ex Benn.) Rob. in rodents: A lesser known ethnomedicinal plant from the Cholanaickan tribal community, India

chronic toxicological studies of Ajuga iva in experimental animals. J Ethnopharmacol 91 : 43–50. Hoffmann D, Adler M, Vaidya VS, Rached E, Mulrane L, Gallagher WM, Callanan JJ, Gautier JC, Matheis K, Staedtler F, Dieterle F, Brandenburg A, Sposny A, Hewitt P, Ellinger-Ziegelbauer H, Bonventre JV, Dekant W, Mally A. (2010). Performance of novel kidney Biomarkers in preclinical toxicity studies. Toxicol Sci 116: 8–22. Kakkar P, Das B, Viswanathan PN. (1984). A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 21

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Impact of quercetin on tight junctional proteins and BDNF signaling molecules in hippocampus of PCBs-exposed rats

). Neuroprotective effects of quercetin and rutin on spatial memory impairment in an 8-arm radial maze task and neuronal death induced by repeated cerebral ischemia in rats. J Pharmacol Sci 104 (4): 329–34. Puntarulo S, Cederbaum AI. (1988). Increased microsomal interaction with iron and oxygen radical generation after chronic acetone treatment. Biochim Biophys Acta 964 (1): 46–52. Roegge CS, Seo BW, Crofton KM, Schantz SL. (2000). Gestational-lactational exposure to Aroclor 1254 impairs radial-arm maze performance in male rats. Toxicol Sci 57 (1): 121

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Antiproliferative, neurotoxic, genotoxic and mutagenic effects of toxic cyanobacterial extracts

cyanobacterial microcys-tins using high performance tandem mass spectrometry. J Am Soc Mass Spectrom 25 : 1253–62. Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY. (1979). Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Microbiology 111 : 1–61. Romero-Jiménez M, Campos-Sánchez J, Analla M, Muñoz-Serrano A, Alonso-Moraga Á. (2005). Genotoxicity and anti-genotoxicity of some traditional medicinal herbs. Mutat Res Genet Toxicol Environ Mutagen 585 : 147–55. Sainis I, Fokas D, Vareli K, Tzakos AG

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Preparation and performance of a colorimetric biosensor using acetylcholinesterase and indoxylacetate for assay of nerve agents and drugs

ABSTRACT

Different toxic compounds can target the cholinergic nervous system. Acetylcholinesterase (AChE; EC 3.1.1.7) is one of the most crucial components of the cholinergic nervous system and thus many of the toxins interact with this enzyme. As to inhibitors, nerve agents used as chemical warfare, some insecticides, and drugs influencing the cholinergic system are common examples of AChE inhibitors. Once inhibited by a neurotoxic compound, a serious cholinergic crisis can occur. On the other hand, sensitivity of AChE to the inhibition can be used for analytical purposes. In this study, a simple disposable biosensor with AChE as a recognition element was devised. AChE was immobilized onto a cellulose matrix and indoxylacetate was used as a chromogenic substrate. The enzyme reaction was assessed by the naked eye using arbitrary units and pyridostigmine, tacrine, paraoxon, carbofuran, soman and VX were assayed as selected inhibitors. A good stability of the biosensors was found, with no aging over a quarter of a year and minimal sensitivity to the interference of organic solvents. The limit of detection ranged from 10 to 100 nmol/L for the compounds tested with a sample volume of 40 μL

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Biochemical aspects of KB-28 compound on physically loaded study subjects

motor urgency during exercise. Sports Med. 2013;43:227-241. https://doi.org10.1007/s40279-013-0025-1 4. Wilmor JH, Costill DI. Physiology of sport and exercise . Illinois: Human Kinetics; 2004:726 5. Lukyanchuk VD, Simonova IV. Actoprotectors: pharmacology and pharmacotherapy. Pharmacol Med Toxicol. 2015;2:14-26 6. Oliynyk S, Oh S. The Pharmacology of Actoprotectors: Practical Application for Improvement of Mental and Physical Performance. Biomols Ther . 2012;20(5):446-56. https://doi.org10.4062/biomolther.2012.20.5.446 7. Kolomoets OS

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Pyridoindole SMe1EC2 as cognition enhancer in ageing-related cognitive decline

radicals in canine counterpart of senile dementia of the Alzheimer type. J Exp Gerontol 38 (6): 711–9. Socci DJ, Crandall BM, Arendash GW. (1995). Chronic antioxidant treatment improves the cognitive performance of aged. Rats Brain Research 693 : 88–94. Spitzer N, Sammons GS, Price EM. (2011). Autofluorescent cells in rat brain can be convincing impostors in green fluorescent reporter studies. J Neurosci Methods 197 (1): 48–55. Stolc S, Snirc V, Gajdosikova A, Gajdosik A, Gasparova Z, Ondrejickova O, Sotnikova R, Viola A, Rapta P, Jariabka P

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Rat cytochromes P450 oxidize 3-aminobenzanthrone, a human metabolite of the carcinogenic environmental pollutant 3-nitrobenzanthrone

Rat cytochromes P450 oxidize 3-aminobenzanthrone, a human metabolite of the carcinogenic environmental pollutant 3-nitrobenzanthrone

3-Aminobenzanthrone (3-ABA) is a human metabolite of carcinogenic 3-nitrobenzanthrone (3-NBA), which occurs in diesel exhaust and air pollution. Understanding which cytochrome P450 (CYP) enzymes are involved in metabolic activation and/or detoxication of this toxicant is important in the assessment of an individual's susceptibility to this substance. The aim of this study was to evaluate the efficiency of rat hepatic CYPs to oxidize 3-ABA and to examine the metabolites formed during such an oxidation. The metabolites formed by CYPs in rat hepatic microsomes were separated by high performance liquid chromatography (HPLC). 3-ABA is oxidized by these enzymes to three metabolites, which were separated by HPLC as distinguish product peaks. Using co-chromatography with synthetic standards, two of them were identified to be oxidative metabolites of 3-ABA, N-hydroxy-3-ABA and 3-NBA. The structure of another 3-ABA metabolite remains to be characterized. To define the role of rat hepatic CYP enzymes in metabolism of 3-ABA, we investigated the modulation of its oxidation using different inducers of CYPs for treatment of rats to enrich the liver microsomes with individual CYPs. Based on these studies, we attribute most of 3-ABA oxidation in rat hepatic microsomes to CYP2B, followed by CYP1A, although a role of other hepatic CYPs cannot be ruled out. Inhibition of 3-ABA oxidation by selective inhibitors of individual CYPs, supported this finding.

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Review Article. Technical aspects of oxygen level regulation in primary cell cultures: A review

Abstract

Oxygen (O2) is an essential element for aerobic respiration. Atmospheric concentration of O2 is approximately 21%. Mammalian cells, however, are generally adapted to O2 levels much lower than atmospheric conditions. The pericellular levels of O2 must also be maintained within a fairly narrow range to meet the demands of cells. This applies equally to cells in vivo and cells in primary cultures. There has been growing interest in the performance of cell culture experiments under various O2 levels to study molecular and cellular responses. To this end, a range of technologies (e.g. gas-permeable technology) and instruments (e.g. gas-tight boxes and gas-controlled incubators) have been developed. It should be noted, however, that some of these have limitations and they are still undergoing refinement. Nevertheless, better results should be possible when technical concerns are taken into account. This paper aims to review various aspects of O2 level adjustment in primary cell cultures, regulation of pericellular O2 gradients and possible effects of the cell culture medium.

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