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Higher levels of total pepsin and bile acids in the saliva as a possible risk factor for early laryngeal cancer

. Quadeer MA, Lopez R, Wood BG. Does acid suppresive therapy reduce the risk of laryngeal cancer recurrence? Laryngoscope 2005; 115: 1877-81. 8. Copper MP, Smit CF, Stanojcic LD, Devriese PP, Schouwenburg PF Mathus- Vliegen LM. High incidence of laryngeal reflux in patients with head and neck cancer. Laryngoscope 2000; 110: 1007-11. 9. Belafsky PC, Postma GN, Koufman JA. Validity and reliability of the reflux Symptom Index (RSI). J Voice 2002; 16: 274-7. 10. Samuels TL, Johnston N. Pepsin as a marker of extraesophageal

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Salivary Theranostics in Pediatric and Special Care Dentistry

metallic dental materials. Comput Appl Electr Eng, 2015;13:143-152. 21. Hashizume N, Fukahori S, Asagiri K, Ishii S, Saikusa N, Higashidate N et al. The characteristics of salivary pepsin in patients with severe motor and intellectual disabilities. Brain Dev, 2017;39:703-709. 22. Reddihough D. Management of drooling in neurological disabilities: more evidence is needed. DMCN, 2017;59:460-461. 23. Siqueira, WL, Bermejo PR, Mustacchi Z. Buffer capacity, pH, and flow rate in saliva of children aged 2–60 months with Down syndrome. Clin Oral Invest, 2005

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What’s new about symptomatic reflux disease

appropriate controls, of gastric acid and pepsin secretion in clinical esophagitis. Gastroenterol. 1991;101:1149-58. 10. Bardhan KD, Muller-Lissner S, Bigard MA, et al. Symptomatic gastrooesophageal reflux disease: double blind controlled study of intermittent treatment with omeprazole or ranitidine. The European Study Group. BMJ. 1999;318:502-7. 11. Miner P Jr, Katz PO, Chen Y, et al. Gastric acid control with esomeprazole, lansoprazole, omeprazole, pantoprazole, and rabeprazole: a fiveway crossover study. Am J Gastroenterol 2003

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Type I collagen extracted from rat-tail and bovine Achilles tendon for dental application: a comparative study


Background: Collagen has attracted great interest as a biomaterial for various dental and medical uses.

Objective: Investigate the characteristics and biocompatibility of type I collagen extracted from rat-tail tendon and bovine Achilles tendon for dental application.

Materials and methods: Type-I collagen was extracted from rat-tail and bovine Achilles tendon using pepsin. The purity of collagen extracts was examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The biocompatibility with human gingival fibroblasts (HGFs) and human oral keratinocytes (HOKs) was examined using an MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Scanning electron microscope (SEM) illustrations of purified collagen alone and collagen with HGFs and HOKs were presented. A three-dimensional wound-healing model of fibroblast populated collagen lattice (FPCL) was used to determine the capability of both sources of collagen to induce wound healing in vitro. Cellular collagen lattices were fabricated to examine the contraction rate of these collagens.

Results: The average yield of collagen extracted from rat-tail and bovine Achilles tendon were 21.8±14.9% and 5.4±0.4%, respectively. The SDS-PAGE analysis showed that the extracts were composed of alpha 1, alpha 2 and beta chains with little contamination of other small proteins. The MTT assay showed good proliferation of cells cultured with each collagen extract, indicating that collagen extracts were non-toxic to the cells. SEM and the FPCL analysis showed that both types of collagen were biocompatible with both HGFs and HOKs, inducing good contraction in the in vitro model.

Conclusion: Type-I collagen extracted from rat-tail and bovine Achilles tendon appeared to be biocompatible with HGFs and HOKs. Both biomaterials may be of use in dental practice.

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Swallowing disorders after treatment for head and neck cancer

. 23 An interesting finding of the present study is a connection between dysphagia and the symptoms of gastroesophageal reflux. Reflux of gastric acid and pepsin is well-recognized etiologic factor in dysphagia even in subjects without cancer, simply by causing inflammation of the laryngeal and hypopharyngeal mucosa. 24 , 25 Such inflammation can additionally aggravate pre-existing radiomucositis in irradiated patients, leading to scarring and impaired sensitivity of involved mucosa. Reflux to the level above the upper esophageal sphincter was found to impair the

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Adsorption and inactivation of proteolytic enzymes by Triaenophorus nodulosus (Cestoda)

parasitizing the fi sh intestine on the activity of the host proteinases. Biol. Bull., 43: 146 - 151. DOI: 10.1134/S1062359016010076 KAGEYAMA, T. (1998): Molecular cloning, expression and characterization of an Ascaris inhibitor for pepsin and cathepsin E. Eur. J. Biochem., 253: 804 - 809. DOI: 10.1046/j.1432-1327.1998.2530804.x KLIMENKO, V., ĶĔNIŅA, V. (1971): On the influence of phylogeny and ecology on some biochemical mechanisms of helminth adaptation. Latvijas Zinatnu akademijas vestis, 11: 93 - 96. (In Russian) KNOX, D

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Diabetes-mediated changes in rat type i collagen and spermatogenesis indices

Biochem 71:114-118, 1976 12. Rubin AL, Drake MP, Davison PF et al. Effect of pepsin on the interaction properties of tropocollagen macromolecules. Biochemistry 4: 181-190, 1965. 13. Maurer G. The Disk-electrophoresis, Mir, Moscow, p. 247, 1971 (in Russian). 14. Deveni T, Gherghey J. The amino-acids, peptides and proteins, Mir, Moscow, p. 364, 1976 (in Russian). 15. Boekelheide K, Chapin R. Male reproductive toxicology . In Current Protocols in Toxicology , Costa LG, Hodgson E, Lawrence DA, Ozolins

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Research Note. Prevalence, protein analysis and possible preventive measures against zoonotic anisakid larvae isolated from marine Atherina fish

, C.V. (2004): Infections related to the ingestion of seafood. Part II: parasitic infections and food safety. Lancet Infect. Dis., 4: 294 - 300. DOI: 10.1016/S1473-3099(04)01005-9 CABALLERO, M.L., MONEO, I. (2004): Several allergens from Anisakis simplex are highly resistant to heat and pepsin treatments. Parasitol. Res., 93: 248 - 251. DOI: 10.1007/s00436-004-1099-3 CARBALLEDA-SANGIAO, N., OLIVARES, F., RODRIGUEZ-MAHILLO, A.I., CARECHE, M., TEJADA, M., MONEO, I., GONZALEZ-MUNOZ, M. (2014): Identifi cation of autoclave

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Review article. Diagnosis and management of venomous snakebites in Southeast Asia

between safety and biochemical characteristics of antivenoms. Toxicon. 1999; 37:895-908. 30. Otero-Patiño R, Cardoso JL, Higashi HG, Nunez V, Diaz A, Toro MF, et al. A randomized, blinded, comparative trial of one pepsin-digested and two whole IgG antivenoms for Bothrops snake bites in Uraba, Colombia. The Regional Group on Antivenom Therapy Research (REGATHER). Am J Trop Med Hyg. 1998; 58:183-9. 31. Boyer LV, Seifert SA, Cain JS. Recurrence phenomena after immunoglobulin therapy for snake envenomations: Part 2. Guidelines for clinical

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Detrimental effects of geldanamycin on adults and larvae of Trichinella spiralis

were obtained from infected stock Swiss mice by 1 % pepsin/1 % HCl digestion ( Dunn & Wright, 1985 ). Two groups of seven animals received GA at a dose of 0.5 and 1 mg/ kg by intraperitoneal injection once every other day to give a total of 3 doses. Treatment started at 7 th day p.i. The remaining mice served as a control infected group. Additional seven infected mice received only DMSO. At 35 days p.i., the animals were euthanized, and muscle larvae were recovered and counted as described before ( Dunn & Wright, 1985 ). Similar parts of the diaphragm and thigh

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