Maja Sereg-Bahar, Ales Jerin and Irena Hocevar-Boltezar
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Ganna M. Shayakhmetova, Larysa B. Bondarenko, Valentina M. Kovalenko and Volodymyr V. Ruschak
, Catanese VM, Rubin DF. Collagen fractionation: separation of native types I, II and III by differential precipitation. Anal Biochem 1976;71:114-8. doi: 10.1016/0003-2697(76)90016-6
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Samuel O. Onoja, Osborn Chinagorom, Chinyere B. C. Ikpa, Kelechi G. Madubuike, Ihechiluru I. Ezeigbo, Solomon N. Ijioma, Aruh O. Anaga and Maxwell I. Ezeja
-induced ulcer model. Misoprostol is a prostaglandin analogue that stimulate mucus and bicarbonate production, inhibit gastric acid secretion and cause vasodilation which shield the stomach mucosa against damage ( 34 ). This suggests that both ESE and misoprostol conferred cytoprotective effect on gastric mucosa and could be the possible mechanism of action. The mucus-bicarbonate layer create a near neutral microenvironment (pH ̴7) at the mucus-mucosa interface and also prevent the proteolytic digestion of gastric epithelium by pepsin ( 35 ). It was observed that ESE caused
Mihaela-Doina Niculescu, Doru-Gabriel Epure, Magdalena Lasoń-Rydel, Carmen Gaidau, Mihai Gidea and Cristina Enascuta
sources and potential cosmetic applications J Cosmet Dermatol 2018 17 1 20 26
2 Delgado LM, Shologu N, Fuller K, Zeugolis DI. Acetic acid and pepsin result in high yield, high purity and low macrophage response collagen for biomedical applications. Biomed Mater 2017; 12(6): article no. 065009.
Delgado LM Shologu N Fuller K Zeugolis DI Acetic acid and pepsin result in high yield, high purity and low macrophage response collagen for biomedical applications Biomed Mater 2017 12 6 article no. 065009
3 Fu W, Liu ZL, and al
Suteera Techatanawat, Rudee Surarit, Theeralaksna Suddhasthira and Siribang-on Piboonniyom Khovidhunkit
Background: Collagen has attracted great interest as a biomaterial for various dental and medical uses.
Objective: Investigate the characteristics and biocompatibility of type I collagen extracted from rat-tail tendon and bovine Achilles tendon for dental application.
Materials and methods: Type-I collagen was extracted from rat-tail and bovine Achilles tendon using pepsin. The purity of collagen extracts was examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The biocompatibility with human gingival fibroblasts (HGFs) and human oral keratinocytes (HOKs) was examined using an MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Scanning electron microscope (SEM) illustrations of purified collagen alone and collagen with HGFs and HOKs were presented. A three-dimensional wound-healing model of fibroblast populated collagen lattice (FPCL) was used to determine the capability of both sources of collagen to induce wound healing in vitro. Cellular collagen lattices were fabricated to examine the contraction rate of these collagens.
Results: The average yield of collagen extracted from rat-tail and bovine Achilles tendon were 21.8±14.9% and 5.4±0.4%, respectively. The SDS-PAGE analysis showed that the extracts were composed of alpha 1, alpha 2 and beta chains with little contamination of other small proteins. The MTT assay showed good proliferation of cells cultured with each collagen extract, indicating that collagen extracts were non-toxic to the cells. SEM and the FPCL analysis showed that both types of collagen were biocompatible with both HGFs and HOKs, inducing good contraction in the in vitro model.
Conclusion: Type-I collagen extracted from rat-tail and bovine Achilles tendon appeared to be biocompatible with HGFs and HOKs. Both biomaterials may be of use in dental practice.
Martina Pezdirec, Primoz Strojan and Irena Hocevar Boltezar
An interesting finding of the present study is a connection between dysphagia and the symptoms of gastroesophageal reflux. Reflux of gastric acid and pepsin is well-recognized etiologic factor in dysphagia even in subjects without cancer, simply by causing inflammation of the laryngeal and hypopharyngeal mucosa. 24 , 25 Such inflammation can additionally aggravate pre-existing radiomucositis in irradiated patients, leading to scarring and impaired sensitivity of involved mucosa. Reflux to the level above the upper esophageal sphincter was found to impair the
parasitizing the fi sh intestine on the activity of the host proteinases. Biol. Bull., 43: 146 - 151. DOI: 10.1134/S1062359016010076
KAGEYAMA, T. (1998): Molecular cloning, expression and characterization of an Ascaris inhibitor for pepsin and cathepsin E. Eur. J. Biochem., 253: 804 - 809. DOI: 10.1046/j.1432-1327.1998.2530804.x
KLIMENKO, V., ĶĔNIŅA, V. (1971): On the influence of phylogeny and ecology on some biochemical mechanisms of helminth adaptation. Latvijas Zinatnu akademijas vestis, 11: 93 - 96. (In Russian)
Larysa B. Bondarenko, Ganna M. Shayakhmetova, Anatoliy V. Matvienko and Valentina M. Kovalenko
Biochem 71:114-118, 1976
12. Rubin AL, Drake MP, Davison PF et al. Effect of pepsin on the interaction properties of tropocollagen macromolecules. Biochemistry 4: 181-190, 1965.
13. Maurer G. The Disk-electrophoresis, Mir, Moscow, p. 247, 1971 (in Russian).
14. Deveni T, Gherghey J. The amino-acids, peptides and proteins, Mir, Moscow, p. 364, 1976 (in Russian).
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