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Using in vitro methods to estimate metabolizable energy content of five forage legumes harvested under different defoliation systems / Einsatz von in vitro Methoden zur Schätzung der umsetzbaren Energie in fünf Futterleguminosen aus unterschiedlichen Nutzungssystemen

Abbreviations OMD, organic matter digestibility; TT, Tilley and Terry method; CM, pepsin-cellulase method; ME, metabolizable energy; WC, white clover; RC, red clover; LC, lucerne; BT, birdsfoot trefoil; KC, kura clover; RG, rotational grazing; CM-OMD, OMD based on pepsin-cellulase method; TTOMD, OMD based on Tilley and Terry method; ME CM , ME estimated by pepsin-cellulase method; ME TT , ME estimated by Tilley and Terry method; MAT, mean average temperature; AP, average precipitation. 1 Introduction With the genetic advancement of new cultivars

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Antioxidative Properties of Bee Pollen Extracts Examined by EPR Spectroscopy

Antioxidative Properties of Bee Pollen Extracts Examined by EPR Spectroscopy

Bee pollen is a valuable and highly recognized source of exogenous antioxidants. The aim of these studies was to determine the antioxidant capacity of three types of bee pollen extracts: ethanol extracts of bee pollen, pepsin extracts of bee pollen and ethanol extracts of pepsin-digested bee pollen. Their antioxidant properties were determined with the use of electron paramagnetic resonance (EPR) and their ability to quench DPPH free radicals was estimated. The EPR results showed that ethanol extracts of pepsin-digested bee pollen (EEPP) had the highest antioxidative effect and the highest free radical DPPH scavenging potential. The pepsin extracts of bee pollen (PEP) had the weakest antioxidant capacity. The ability to quench DPPH free radicals was also the weakest one for this extract. An average antioxidative effect was recorded for ethanol extracts of bee pollen (EEP).

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Body-Surface Compounds in Buckfast and Caucasian Honey Bee Workers (Apis Mellifera)

References Andersen S. O. (1979) Biochemistry of insect cuticle. Annual Review of Entomology 24: 29-61. DOI: 10.1146/ annurev.en.24.010179.000333 Anson M. (1938) The estimation of pepsin, tripsin, papain and cathepsin with hemoglobin. The Journal of General Physiology 22: 79-84. Arnold G., Quenet B., Cornuet J. M., Masson C., DeSchepper B., Estoup A., Gasquil P. (1996) Kin recognition in honeybees. Nature 379: 498. DOI:10.1038/379498a0 Borsuk G., Olszewski K. (2010) Morphometric traits of

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Curcumin Stimulates Biochemical Mechanisms of Apis Mellifera Resistance and Extends the Apian Life-Span

., Misra K., Priyadarsini I., Rajasekharan K., Aggarwal B. (2008) Biological activities of curcumin and its analogues (Congeners) made by man and Mother Nature. Biochemical Pharmacology 76: 1590-1611. DOI: 10.1016/j.bcp.2008.08.008 Anson M. (1938) The estimation of pepsin, tripsin, papain and cathepsin with hemoglobin. Journal of General Physiology 22: 79-84. Arshami J., Pilevar M., Azghadi M., Raji A. (2012) Hypolipidemic and antioxidative effects of curcumin on blood parameters, humoral immunity, and jejunum histology in Hy-line hens

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Effect of pre-treatment on the generation of dipeptidyl peptidase-IV- and prolyl endopeptidase-inhibitory hydrolysates from bovine lung

efficacy of a number of enzymes, including Alcalase, Protamex, Savinase and Corolase 7089, on lentil proteins ( Garcia-Mora et al ., 2015 ). Moreover, high-pressure treatments also increased proteolysis of ovalbumin with pepsin, trypsin and chymotrypsin ( Quirós et al ., 2007 ) and proteolysis of bovine collagen with Alcalase, collagenase, thermolysin, pepsin and trypsin ( Zhang et al ., 2013 ). Although only one high-pressure pre-treatment was tested in this study (600 MPa, 3 min), a previous study carried out by Singh and Ramaswamy (2014) suggested that the DH was

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Gastroprotective effects of methanol extract of Eremomastax speciosa leaf harvested in Southern part of Nigeria in rat

-induced ulcer model. Misoprostol is a prostaglandin analogue that stimulate mucus and bicarbonate production, inhibit gastric acid secretion and cause vasodilation which shield the stomach mucosa against damage ( 34 ). This suggests that both ESE and misoprostol conferred cytoprotective effect on gastric mucosa and could be the possible mechanism of action. The mucus-bicarbonate layer create a near neutral microenvironment (pH ̴7) at the mucus-mucosa interface and also prevent the proteolytic digestion of gastric epithelium by pepsin ( 35 ). It was observed that ESE caused

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Biocomposites based on collagen and keratin with properties for agriculture and industrie applications

Collagen: A review on its sources and potential cosmetic applications J Cosmet Dermatol 2018 17 1 20 26 2 Delgado LM, Shologu N, Fuller K, Zeugolis DI. Acetic acid and pepsin result in high yield, high purity and low macrophage response collagen for biomedical applications. Biomed Mater 2017; 12(6): article no. 065009. 28767045 Delgado LM Shologu N Fuller K Zeugolis DI Acetic acid and pepsin result in high yield, high purity and low macrophage response collagen for biomedical applications Biomed Mater 2017 12 6 article no. 065009

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Adsorption and inactivation of proteolytic enzymes by Triaenophorus nodulosus (Cestoda)

parasitizing the fi sh intestine on the activity of the host proteinases. Biol. Bull., 43: 146 - 151. DOI: 10.1134/S1062359016010076 KAGEYAMA, T. (1998): Molecular cloning, expression and characterization of an Ascaris inhibitor for pepsin and cathepsin E. Eur. J. Biochem., 253: 804 - 809. DOI: 10.1046/j.1432-1327.1998.2530804.x KLIMENKO, V., ĶĔNIŅA, V. (1971): On the influence of phylogeny and ecology on some biochemical mechanisms of helminth adaptation. Latvijas Zinatnu akademijas vestis, 11: 93 - 96. (In Russian) KNOX, D

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Research Note. Prevalence, protein analysis and possible preventive measures against zoonotic anisakid larvae isolated from marine Atherina fish

, C.V. (2004): Infections related to the ingestion of seafood. Part II: parasitic infections and food safety. Lancet Infect. Dis., 4: 294 - 300. DOI: 10.1016/S1473-3099(04)01005-9 CABALLERO, M.L., MONEO, I. (2004): Several allergens from Anisakis simplex are highly resistant to heat and pepsin treatments. Parasitol. Res., 93: 248 - 251. DOI: 10.1007/s00436-004-1099-3 CARBALLEDA-SANGIAO, N., OLIVARES, F., RODRIGUEZ-MAHILLO, A.I., CARECHE, M., TEJADA, M., MONEO, I., GONZALEZ-MUNOZ, M. (2014): Identifi cation of autoclave

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Detrimental effects of geldanamycin on adults and larvae of Trichinella spiralis

were obtained from infected stock Swiss mice by 1 % pepsin/1 % HCl digestion ( Dunn & Wright, 1985 ). Two groups of seven animals received GA at a dose of 0.5 and 1 mg/ kg by intraperitoneal injection once every other day to give a total of 3 doses. Treatment started at 7 th day p.i. The remaining mice served as a control infected group. Additional seven infected mice received only DMSO. At 35 days p.i., the animals were euthanized, and muscle larvae were recovered and counted as described before ( Dunn & Wright, 1985 ). Similar parts of the diaphragm and thigh

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