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Using in vitro methods to estimate metabolizable energy content of five forage legumes harvested under different defoliation systems / Einsatz von in vitro Methoden zur Schätzung der umsetzbaren Energie in fünf Futterleguminosen aus unterschiedlichen Nutzungssystemen

Abbreviations OMD, organic matter digestibility; TT, Tilley and Terry method; CM, pepsin-cellulase method; ME, metabolizable energy; WC, white clover; RC, red clover; LC, lucerne; BT, birdsfoot trefoil; KC, kura clover; RG, rotational grazing; CM-OMD, OMD based on pepsin-cellulase method; TTOMD, OMD based on Tilley and Terry method; ME CM , ME estimated by pepsin-cellulase method; ME TT , ME estimated by Tilley and Terry method; MAT, mean average temperature; AP, average precipitation. 1 Introduction With the genetic advancement of new cultivars

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Evaluation of the ACE-Inhibitory Activity of Egg-White Proteins Degraded with Pepsin

Increasing the potency of antihypertensive food-derived peptides is a critical and important step in the development of natural drugs for cardiovascular diseases prevention. We have proposed the egg-white protein precipitate (EWPP) obtained as a byproduct of cystatin and lysozyme isolation as a potential source of ACE-inhibitory peptides derived by pepsin digestion. The results indicated that hydrolysis of EWPP with pepsin produced the ACE inhibitory activity. During 3-h hydrolysis (DH: 38.3%), the IC50 value of EWPP hydrolysate was significantly increased and finally reached IC50=643.1 μg/mL. This hydrolysate was further fractionated by RP-HPLC. The peptide fraction exhibiting the highest ACE inhibitory activity was rechromatographed. Three peptide subfractions exhibiting ACE-inhibitory activities of 69.0, 25.0, and 37.6 μg/mL were further characterised. In each of them, mixtures of peptides with different molecular masses were observed.

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Healthy Multifunctional Spectra of Milk Glycoproteins and Their Fragments - a Review Article

K., Tomita M., Giehl T.J., Ellison R.T., Antibacterial activity of lactoferrin and a pepsin-derived lactoferrin peptide fragment. Inf. Immun., 1993, 61, 719-728. Ye X.Y., Wang H.X., Liu F., Ng T.B., Ribonuclease, cell-free translation-inhibitory and superoxide radical scavenging activities of the iron-binding protein lactoferrin from bovine milk. Int. J. Biochem. Cell. Biol., 2000, 32, 235-241. Yoo Y.C., Watanabe R., Koike Y., Mitobe M., Shimazaki K., Watanabe S., Azuma I., Apoptosis in human leukemic cells

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SE-HPLC-DAD Analysis of Flaxseed Lignan Macromolecule and its Hydrolysates

SE-HPLC-DAD Analysis of Flaxseed Lignan Macromolecule and its Hydrolysates

A lignan macromolecule (LM) was extracted from defatted flaxseeds using an ethanol-dioxan system (1:1, v/v) and purified using Amberlite column chromatography with water and methanol as mobile phases. The LM was subjected to chemical hydrolysis (base, acid, base & acid), as well as to enzymatic processing using pepsin, pancreatin, cellulase, and β-glucuronidase.

The study revealed that lignan macromolecule in flaxseed was not homogenous. The chemical hydrolysis as well as enzymatic treatment using β-glucuronidase and cellulase released low molecular phenolic compounds from the lignan macromolecule. The liberation of secoisolariciresinol (SECO) and free phenolic acids (p-coumaric and ferulic acids) from flaxseed lignan macromolecule as a result of the base and acid hydrolyses was noted. The application of pepsin and pancreatin did not change the composition of the lignan macromolecule.

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Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins

Abstract

Protein digestibility may be influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purified rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose-dependently decreased upon addition of hull fibres at all inclusion levels (8.9-26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only significant at the medium to high levels (7.3-8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level.

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Antioxidant Activity of Hydrolysates Prepared from Flaxseed Cake Proteins Using Pancreatin

. Valdez-Ortiz A., Fuentes-Gutierrez CI., German-Baez L.J. Gutierrez-Dorado R, Medina-Godoy S., Protein hydrolysates obtained from Azufrado (sulphur yellow) beans (Phaseo-lus vulgaris): Nutritional, ACE-inhibitory and antioxidative characterization. LWT-Food Sci. Technol., 2012, 46, 91-96. 35. Zambrowicz A., Timmer M., Eckert E., Trziszka T, Evaluation of the ACE-inhibitory activity of egg-white proteins degraded with pepsin. Pol. J. Food Nutr. Sci., 2013, 63, 103-108. 36. Zhang M.-N, Huang G.-R, Jiang J.-X., Iron binding capacity of dephytinised soy protein

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Adsorption and inactivation of proteolytic enzymes by Triaenophorus nodulosus (Cestoda)

parasitizing the fi sh intestine on the activity of the host proteinases. Biol. Bull., 43: 146 - 151. DOI: 10.1134/S1062359016010076 KAGEYAMA, T. (1998): Molecular cloning, expression and characterization of an Ascaris inhibitor for pepsin and cathepsin E. Eur. J. Biochem., 253: 804 - 809. DOI: 10.1046/j.1432-1327.1998.2530804.x KLIMENKO, V., ĶĔNIŅA, V. (1971): On the influence of phylogeny and ecology on some biochemical mechanisms of helminth adaptation. Latvijas Zinatnu akademijas vestis, 11: 93 - 96. (In Russian) KNOX, D

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Research Note. Prevalence, protein analysis and possible preventive measures against zoonotic anisakid larvae isolated from marine Atherina fish

, C.V. (2004): Infections related to the ingestion of seafood. Part II: parasitic infections and food safety. Lancet Infect. Dis., 4: 294 - 300. DOI: 10.1016/S1473-3099(04)01005-9 CABALLERO, M.L., MONEO, I. (2004): Several allergens from Anisakis simplex are highly resistant to heat and pepsin treatments. Parasitol. Res., 93: 248 - 251. DOI: 10.1007/s00436-004-1099-3 CARBALLEDA-SANGIAO, N., OLIVARES, F., RODRIGUEZ-MAHILLO, A.I., CARECHE, M., TEJADA, M., MONEO, I., GONZALEZ-MUNOZ, M. (2014): Identifi cation of autoclave

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Detrimental effects of geldanamycin on adults and larvae of Trichinella spiralis

were obtained from infected stock Swiss mice by 1 % pepsin/1 % HCl digestion ( Dunn & Wright, 1985 ). Two groups of seven animals received GA at a dose of 0.5 and 1 mg/ kg by intraperitoneal injection once every other day to give a total of 3 doses. Treatment started at 7 th day p.i. The remaining mice served as a control infected group. Additional seven infected mice received only DMSO. At 35 days p.i., the animals were euthanized, and muscle larvae were recovered and counted as described before ( Dunn & Wright, 1985 ). Similar parts of the diaphragm and thigh

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