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Antioxidative Properties of Bee Pollen Extracts Examined by EPR Spectroscopy

Antioxidative Properties of Bee Pollen Extracts Examined by EPR Spectroscopy

Bee pollen is a valuable and highly recognized source of exogenous antioxidants. The aim of these studies was to determine the antioxidant capacity of three types of bee pollen extracts: ethanol extracts of bee pollen, pepsin extracts of bee pollen and ethanol extracts of pepsin-digested bee pollen. Their antioxidant properties were determined with the use of electron paramagnetic resonance (EPR) and their ability to quench DPPH free radicals was estimated. The EPR results showed that ethanol extracts of pepsin-digested bee pollen (EEPP) had the highest antioxidative effect and the highest free radical DPPH scavenging potential. The pepsin extracts of bee pollen (PEP) had the weakest antioxidant capacity. The ability to quench DPPH free radicals was also the weakest one for this extract. An average antioxidative effect was recorded for ethanol extracts of bee pollen (EEP).

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Body-Surface Compounds in Buckfast and Caucasian Honey Bee Workers (Apis Mellifera)

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Curcumin Stimulates Biochemical Mechanisms of Apis Mellifera Resistance and Extends the Apian Life-Span

., Misra K., Priyadarsini I., Rajasekharan K., Aggarwal B. (2008) Biological activities of curcumin and its analogues (Congeners) made by man and Mother Nature. Biochemical Pharmacology 76: 1590-1611. DOI: 10.1016/j.bcp.2008.08.008 Anson M. (1938) The estimation of pepsin, tripsin, papain and cathepsin with hemoglobin. Journal of General Physiology 22: 79-84. Arshami J., Pilevar M., Azghadi M., Raji A. (2012) Hypolipidemic and antioxidative effects of curcumin on blood parameters, humoral immunity, and jejunum histology in Hy-line hens

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Adsorption and inactivation of proteolytic enzymes by Triaenophorus nodulosus (Cestoda)

parasitizing the fi sh intestine on the activity of the host proteinases. Biol. Bull., 43: 146 - 151. DOI: 10.1134/S1062359016010076 KAGEYAMA, T. (1998): Molecular cloning, expression and characterization of an Ascaris inhibitor for pepsin and cathepsin E. Eur. J. Biochem., 253: 804 - 809. DOI: 10.1046/j.1432-1327.1998.2530804.x KLIMENKO, V., ĶĔNIŅA, V. (1971): On the influence of phylogeny and ecology on some biochemical mechanisms of helminth adaptation. Latvijas Zinatnu akademijas vestis, 11: 93 - 96. (In Russian) KNOX, D

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Research Note. Prevalence, protein analysis and possible preventive measures against zoonotic anisakid larvae isolated from marine Atherina fish

, C.V. (2004): Infections related to the ingestion of seafood. Part II: parasitic infections and food safety. Lancet Infect. Dis., 4: 294 - 300. DOI: 10.1016/S1473-3099(04)01005-9 CABALLERO, M.L., MONEO, I. (2004): Several allergens from Anisakis simplex are highly resistant to heat and pepsin treatments. Parasitol. Res., 93: 248 - 251. DOI: 10.1007/s00436-004-1099-3 CARBALLEDA-SANGIAO, N., OLIVARES, F., RODRIGUEZ-MAHILLO, A.I., CARECHE, M., TEJADA, M., MONEO, I., GONZALEZ-MUNOZ, M. (2014): Identifi cation of autoclave

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Use of Duckweed (Lemna L.) in Sustainable Livestock Production and Aquaculture – A Review

. Lassociński W. (1979). Duckweed grown on wastewater as a supplementary source of protein (in Polish). Wszechświat, 10: 232–234. Leng R.A., Stambolie J.H., Bell R. (1995). Duckweed – a potential high-protein feed resource for domestic animals and fish. Livest. Res. Rural Develop., 7: 1. Ly J., Samkol P., Preston T.R. (2002). Nutritional evaluation of aquatic plants for pigs: pepsin/pancreatic digestibility of six plant species. Livest. Res. Rural Develop., 14: 1. Men L.T., Bui H.V., Mai T.C., Preston T.R. (1997). Effect of dietary protein level and duckweed

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Detrimental effects of geldanamycin on adults and larvae of Trichinella spiralis

were obtained from infected stock Swiss mice by 1 % pepsin/1 % HCl digestion ( Dunn & Wright, 1985 ). Two groups of seven animals received GA at a dose of 0.5 and 1 mg/ kg by intraperitoneal injection once every other day to give a total of 3 doses. Treatment started at 7 th day p.i. The remaining mice served as a control infected group. Additional seven infected mice received only DMSO. At 35 days p.i., the animals were euthanized, and muscle larvae were recovered and counted as described before ( Dunn & Wright, 1985 ). Similar parts of the diaphragm and thigh

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