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Comparison of Enzymatic Hydrolysis of Polysaccharides from Eggshells Membranes


AHyaluronic acid (HA) is part of the extracellular matrix of connective, epithelial and neural tissues, as well as the synovial fluid, skin, and cartilage. It is composed of repeating disaccharide units of D-glucuronic acid and N-acetyl glucosamine. Hyaluronic acid is used in abdominal surgery, ophthalmology, dermatology, rhinology; it is usable for the osteoarthritis treatment. The membranes of eggshell are a natural source of hyaluronic acid, collagen, glycosaminoglycan and collagenous proteins. In paper, we tested the possibility of extraction hyaluronic acid from the eggshell membranes by enzymatic hydrolysis. We identified optimal conditions of hydrolysis with trypsin at reaction temperature of 37 °C and pH 8; with pepsin at 40 °C and pH 3, as well as with papain at 60 °C and pH 7.5. The content of hyaluronic acid in samples was determined spectrophotometrically using the carbazole method. The experimental results showed a yield of ~ 4 -4.5 % hyaluronic acid per 1 g of dry eggshell membranes.

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Membrane reactor for enzymatic depolymerization – a case study based on protein hydrolysis

application in obtaining peptides. Chem. Eng. J. 305, 61-68. DOI: 10.106/j.cej.2016.05.087. 15. Labus, K., Trusek-Holownia, A. & Noworyta, A. (2015), Kinetics of protein hydrolysis catalyzed by pepsin. In: 42nd International Conference of Slovak Society of Chemical Engineering proceedings, Tatranské Matliare, Slovakia, May 25-29, 2015. Ed. Jozef Markoš, Slovakia, 465-472.

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Alternative grains in nutrition

-Bensemra, M. C. Courtin, A. J. Delcour, B. Nadjemi, Assessment of Algerian sorghum protein quality [Sorghum bicolor (L.) Moench] using amino acid analysis and in vitro pepsin digestibility. Food Chemistry, 121. (2010) 719–723. [12] T. Németh, A tápanyagellátás hatása a silócirok (Sorghum bicolor L./Moench) tópelem-felvételére, szárazanyag-felhalmozására és terméshozamára. (Effect of nutrient supply on nutrient uptake, dry matter accumulation and yield of sweet sorghum (Sorghum bicolor L./Moench)). Debreceni Egyetem, Novenytudomdnyi Intezet, Debrecen, (2009

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