Hai-Feng Liu, Hui Li, Ge Bai, Qian-Zhen Zhang, Xiang-Gao, Tao Liu and Hong-Bin Wang
housed individually and fed a piglet diet (Shenzhen Jinxinnong Feed, China) and given tap water ad libitum . All pigs were preoperatively assessed as healthy after a physical examination and complete blood count.
Surgical procedure. The pigs were divided into three equal groups: a ⅔ group, a ¾ group, and a ⅚ group. Animals in the ⅔ group were subjected to a ⅓ left nephrectomy and a right radical nephrectomy, animals in the ¾ group had a ½ left nephrectomy and a right radical nephrectomy performed, and animals in the ⅚ group underwent a ⅔ left nephrectomy and a
Bojana B. Ilic, Jadranka A. Antic, Jovana Z. Bankovic, Ivana T. Milicevic, Gordana S. Rodic, Dusan S. Ilic, Cane D. Tulic, Vera N. Todorovic and Svetozar S. Damjanovic
Background: Sporadic clear-cell renal cell carcinoma (ccRCC) is associated with mutations in the VHL gene, upregulated mammalian target of rapamycin (mTOR) activity and glycolytic metabolism. Here, we analyze the effect of VHL mutational status on the expression level of mTOR, eIF4E-BP1, AMPK, REDD1, and PDK3 proteins. Methods: Total proteins were isolated from 21 tumorous samples with biallelic inactivation, 10 with monoallelic inactivation and 6 tumors with a wild-type VHL (wtVHL) gene obtained from patients who underwent total nephrectomy. The expressions of target proteins were assessed using Western blot. results: Expressions of mTOR, eIF4EBP1 and AMPK were VHL independent. Tumors with monoallelic inactivation of VHL underexpressed REDD1 in comparison to wtVHL tumors (P = 0.042), tumors with biallelic VHL inactivation (P < 0.005) and control tissue (P = 0.004). Additionally, REDD1 expression was higher in tumors with VHL biallelic inactivation than in control tissue (P = 0.008). Only in wt tumor samples PDK3 was overexpressed in comparison to tumors with biallelic inactivation of VHL gene (P = 0.012) and controls (P = 0.016). In wtVHL ccRCC, multivariate linear regression analysis revealed that 97.4% of variability in PDK3 expression can be explained by variations in AMPK amount. Conclusion: Expressions of mTOR, eIF4EBP1 and AMPK were VHL independent. We have shown for the first time VHL dependent expression of PDK3 and we provide additional evidence that VHL mutational status affects REDD1 expression in sporadic ccRCC.
Yong Han, Bingyi Shi, Ming Cai, Xiaoguang Xu, Liang Xu, Qiang Wang, Wenqiang Zhou and Li Xiao
CD20 Expression in the Transplanted Kidney of Patients with Graft Loss and Transient Allograft Dysfunction
This study aimed to explore the relationship between the infiltration of CD20+ B cells and the survival time of a renal allograft and to investigate the role of infiltrated B cells in the rejection of the renal allograft. A total of 40 patients with renal allograft loss due to refractory rejection and 20 patients with transient renal allograft dysfunction were recruited. Renal biopsy was done and CD20 expression was detected by immunohistochemistry. In addition, the survival time of the renal allograft was also obtained. The relationships between the CD20 expression and the survival time of the renal allograft and graft loss due to rejection were analyzed. The associations of gender, age and clinicopathogical types with the CD20 expression were also investigated. The proportion of patients positive for CD20 in the transplanted kidney was higher in patients receiving nephrectomy of the allograft due to rejection than in those with transient allograft dysfunction. The diffuse infiltration of CD20+ B cells was considered as positive staining. In 40 samples from patients with graft loss, 19 had diffuse infiltration of CD20+ B cells (47.5%). In 19 patients positive for CD20, hyperacute rejection was found in 1 patient, acute rejection in 5 and chronic rejection in 13. Statistical analysis showed the CD20 expression was not associated with the age and gender of donors and recipients, regimen for immunosuppressive treatment, cold/warm ischemia time and secondary transplantation. CD20+ B cell infiltration predicts a poor prognosis of patients with kidney transplantation and is one of the risk factors of graft loss.
). Indeed, in patients with chronic renal failure, ADMA is considered as a future marker of mortality and cardiovascular disease ( 49 ). SDMA was confirmed as an endogenous marker of renal failure that correlated directly with parameters of renal failure ( 53 , 54 ). After total nephrectomy in a rat model, the circulating level of SDMA increased rapidly in parallel with blood urea nitrogen and creatinine ( 55 ). SDMA can indirectly affect NO synthesis by two mechanisms that depend on interfering with the intracellular uptake of arginine: either by inhibiting renal
Jiangeng Han, Xin Wang, Zheng Chen, Ning Gao and Chen Wang
-negative bacteria, such as fluoroquinolone ketones. CT is the most effective method for the early diagnosis of emphysema pyelonephritis. According to CT findings, emphysema pyelonephritis is divided into types I and II: type I is considered as the dry type characterized by renal parenchymal necrosis with mottled gas without liquid, while type II is considered as the wet type characterized by the presence of gas in the kidney or perirenal fluid accompanied by a bubble gas or in a collection system. Treatment options have some controversy. In the past, emergency nephrectomy is a
Roman Aleksiewicz, Krzysztof Lutnicki, Marcin Bojarski, Ilona Al-Mutari and Aneta Bocheńska
Doppler US measurement in a healthy population. Radiology 1996, 199, 165–169.
12. Kim S.H.: The usefulness of pulsatile flow detection in measuring resistive index in renal Doppler US. Korean J Radiol 2002, 3, 45–48.
13. Kujawa-Szewieczek A., Kolonko A., Chudek J., Więcek A.: No effect of pretransplantation bilateral nephrectomy on the resistive indices measured in the kidney allograft. Transplant Proc 2014, 46, 2602–2605.
14. Lin G.J., Cher T.W.: Renal vascular resistance in normal children - a color Doppler study. Pediatric Nephrol 1997, 11, 182
Ileana Teodoru, Iulian Mincu and Gabriela Radulian
Gerontol A Biol Sci Med Sci 68: 677-681, 2013.
6. Afolabi PR, Jahoor F, Gibson NR, Jackson AA. Response of hepatic proteins to the lowering of habitual dietary proteins to the recommended safe level of intake. Am J Physiol Endocrinol Metab 287: E327-E330, 2004.
7. Addis T, Lew W. Diet and death in acute uremia. J Clin Invest 18: 773-775, 1939.
8. Mackay LL, Addis T, Mackay EM. The degree of compensatory renal hypertrophy following unilateral nephrectomy: II. The influence of the protein intake. J Exp Med 67: 515-519, 1938
Marta Szweda, Andrzej Rychlik, Izabella Babińska and Andrzej Pomianowski
11. Carvalho S., Stoll A.L., Priestnall S.L., Suarez-Bonnet A., Rassnick K., Lynch S., Schoepper I., Romanelli G., Buracco P., Atherton M., de Merlo E.M., Lara-Garcia A..: Retrospective evaluation of COX-2 expression, histological and clinical factors as prognostic indicators in dogs with renal cell carcinomas undergoing nephrectomy. Vet Comp Oncol 2017, 15, 1280–1294.
12. Cetin M., Buyukberber S., Demir M., Sari I., Sari I., Deniz K., Eser B., Altuntaz F., Camci C., Ozturk A., Turgut B., Vural O., Unal A.: Overexpression of cyclooxygenase-2 in multiple