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Antimicrobial susceptibility, plasmid profiles, and RAPD-PCR typing of Acinetobacter bacteria


Background: Multiple-drug resistant Acinetobacter have widely spread in the last decades imposing a serious nosocomial source of infection. Nevertheless, little knowledge was gaimed on tracing the development of antibiotic resistance in Acinetobacter species. Objectives: Explore Acinetobacter spp. via antimicrobial susceptibility, plasmid profiles, and random amplified polymorphism DNA polymerase chain reaction (RAPD-PCR) typing. Methods: One hundred twelve Acinetobacter isolates (including 66 A. baumannii and 46 non-Acinetobacter baumannii strains) were obtained from three university hospitals. The source of infection of these isolates included blood, urine, wound, and respiratory tract. Their susceptibilities to 17 antibiotics were tested and then all Acinetobacter isolates were typed by plasmid analysis and RAPD-PCR method. Results: A. baumannii isolates revealed nine different patterns of antibiotic resistance. Of those, non- A. baumannii, were associated with plasmid and RAPD-PCR typings (p <0.05). A. baumannii was more resistant to multiple antibiotics than non-A. baumannii (p <0.05). Seven different plasmid profiles were observed among 112 Acinetobacter isolates. Plasmids were found in 107 (95.5%) of the 112 isolates. Unlike in RAPD-PCR typing, there was no difference between the type of Acinetobacter, A. or non-A. baumannii strains and plasmid profiles (p >0.05). By RAPD-PCR, six profiles were found for each A. and non-A. baumannii strains. The pattern 6 was the most common pattern among the isolates. Both plasmid and RAPD-PCR typing showed no association between plasmid profiling and site of infection (p >0.05). Conclusion: There is a wide spread of multi-drug resistant Acinetobacter spp., particularly A. baumannii, in the Middle East region that can be traced efficiently by plasmid and genotyping typing of Acinetobacter. More care should be taken for tracing the development of antimicrobial resistance of Acinetobacter using precise molecular typing techniques.

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Fingerprints by pulsed-field gel electrophoresis of leptospires isolated from field rats and comparison with reference Leptospira serovars

polymorphism.Vet Rec. 1993; 132:325-6. 11. Natarajaseenivasan K, Prabhu N, Selvanayaki K, Raja SS, Ratnam S. Human leptospirosis in Erode, South India: serology, isolation, and characterization of the isolates by randomly amplified polymorphic DNA (RAPD) fingerprinting. Jpn J Infect Dis. 2004; 7: 193-7. 12. Perolat P, Grimont F, Regnault B, Grimont PA, Fournie E, Thevenet H, et al. rRNA gene restriction patterns of Leptospira: a molecular typing system. Res Microbiol. 1990; 141:159-71. 13. Kositanont U, Chotinantakul K

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Two Rare Cases Of Central Nervous System Opportunistic Mycoses / Oportunističke Mikoze Središnjega Živčanog Sustava – Prikaz Dvaju Bolesnika

susceptibilities and molecular typing of sequentially isolated clinical Cryptococcus neoformans strains from Croatia. J Med Microbiol 2011;60:1487-95. 14. Murray PR, Rosenthal KS, Pfaller MA. Medical Microbiology. 6th ed. Philadelphia: Mosby Elsevier; 2009. 15. de Hoog GS, Guarro J, Gene J, Figueras MJ. Atlas of Clinical Fungi. 4th ed. Utrecht: CBS; 2011. 16. Anaissie EJ, Stratton SL, Dignani CM, Summerbell RC, Rex JH, Monson TP, Spencer T, Kasai M, Francesconi A, Walsh TJ. Pathogenic Aspergillus species recovered from a

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Reduced susceptibility to disinfectants of Acinetobacter baumannii biofilms on glass and ceramic

interim standard definitions for acquired resistance. Clin Microbiol Infect 2012;18:268-81. doi: 10.1111/j.1469-0691.2011.03570 25. Célia Maria Carvalho Pereira Araújo Romão, Faria YN, Pereira LR, Asensi MD. Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing. Mem Inst Oswaldo Cruz 2005;100:541-8. doi: 10.1590/S0074-02762005000500015 26. Pour NK, Dusane DH, Dhakephalkar PK, Zamin FR, Zinjarde SS, Chopade BA. Biofilm formation by Acinetobacter baumannii strains

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Pulsed-Field Gel Electrophoresis Used for Typing of Extended-Spectrum-β-Lactamases- Producing Escherichia coli Isolated from Infant ҆S Respiratory and Digestive System

.3.59 PMid:16602980 20. Caprioli, A., Maugliani, A., Michelacci, V., Morabito, S. (2014). Molecular typing of Verocytotoxin -producing E. coli (VTEC) strains isolated from food, feed and animals : state of play and standard operating procedures for pulsed field gel electrophoresis (PFGE) typing, profiles interpretation and curation. EFSA journal EN704, 55. 21. Barrett, T.J., Gerner-Smidt, P., Swaminathan, B. (2006). Interpretation of pulsed-field gel electrophoresis patterns in foodborne disease investigations and surveillance. Foodborne Pathog Dis. 3(1): 20

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Occurrence of Cryptosporidium Spp. and Giardia Duodenalis in Red Foxes (Vulpes Vulpes) in Bosnia and Herzegovina

:21744018 11. Hamnes, I. S, Gjerde, B. K., Robertson, L. J. (2007b). A longitudinal study on the occurrence of Cryptosporidium and Giardia in dogs during their first year of life. Acta Vet Scand. 49, 22. PMid:17848186; PMCid:PMC2040143 12. Beck, R., Sprong, H., Bata, I., Lucinger, S., Pozio, E., Cacció, S. M. (2011b). Prevalence and molecular typing of Giardia spp. in captive mammals at the ZOO of Zagreb, Croatia. Vet Parasitol. 175, 40-46. PMid

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Antibiotic resistance, multidrug resistance and enterobacterial repetitive intergenic consensus polymerase chain reaction profiles of clinically important Klebsiella species

Reservoirs of drug resistant bacterial genomes and extrachromosomal DNA segments are a growing problem and cause emergence of new multidrug resistant (MDR) strains [ 1 ]. Antibiotic resistance of Klebsiella infections are causing increasing morbidity and mortality, and an increase in health care costs worldwide. In epidemiological research, not only phenotypical analysis, but also genotypical analysis is conducted by using various molecular typing methods such as plasmid profiling, ribotyping, and polymerase chain reaction (PCR) to find genetic

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Bovine Tuberculosis in the Republic of Macedonia: Postmortem, Microbiological and Molecular Study in Slaughtered Reactor Cattle

.B., Goria, M., Loda, D., Garrone, A., Benedetto, A., Mondo, A., Tisato, E., Zanoni, M., Zoppi, S., Dondo, A., Tagliabue, S., Bonora, S., Zanardi, G., Pacciarini, M.L. (2009). Molecular typing of Mycobacterium bovis strains isolated in Italy from 2000 to 2006 and evaluation of variablenumber- tandem-repeats for a geographic optimized genotyping. J Clin Microbiol. 47 (3): 636-644. PMid:19144792 PMCid:PMC2650904 44. Prodinger, W.M., Brandstätter, A., Naumann, L., Pacciarini, M., Kubica, T., Boschiroli, M.L., Aranaz, A

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Application of Fluorescence Based Molecular Assays for Improved Detection and Typing of Brucella Strains in Clinical Samples

PMCid:PMC264138 18. Bricker, B.J., Halling, S.M. (1995). Enhancement of the Brucella AMOS-PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51. J. Clin. Microbiol., 33, 1640-1642. PMid:7650203 PMCid:PMC228233 19. Lopez-Goni, I., Garcia-Yoldi, D., Marin, C.M., de Miguel, M.J., Muñoz, P.M., Blasco, J.M., Jacques, I., Grayon, M., Cloeckaert, A., Ferreira, A.C., Cardoso, R., Corrêa de Sá, M.I., Walravens, K., Albert, D., Garin-Bastuji, B. (2008). Evaluation of a multiplex PCR assay (bruce-ladder) for molecular

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