infectious diseases are a leading cause of morbidity worldwide ( Rowell et al., 2012 ; Palm et al., 2014).
The aim of this outline is to review the value of strain typing in public health and in the food industry. Selected examples are used to give an overview on current moleculartyping tools, discussing the potential as well as the shortcomings of diverse techniques in reference to our own work and to present an outlook on upcoming technologies based on WGS.
Identification of microorganisms
Identification denotes the assignment of a microorganism into a
K. Sharma, J. Korecký, E.D. Patrizio Soldateschi and P. Sedlák
. Annual Review of Plant Biology, 56, 467-489. doi: 10.1146/annurev.arplant.56.032604.144249.
Tao R, Yamane H, Sugiura A, Murayama H, Sassa H, Mori H (1999): Moleculartyping of S-alleles through identification, characterization and cDNA cloning for S-RNases in sweet cherry. Journal of the American Society for Horticultural Science, 124, 224-233.
Tobutt KR, Sonneveld T, Bekefi Z, Boskovic R (2004): Cherry (in)compatibility genotypes - an updated cultivar table. Acta Hortica, 663, 667-672.
Ushijima K, Sassa H, Tao R, Yamane
Enterobiasis is a human intestinal parasitic disease caused by human pinworm, Enterobius vermicularis. Despite being the most prevalent nematode infection in Europe and North America, predominantly among in school aged children, the data concerning infection rate and knowledge of genetic variability of pinworms are incomplete. The aim of the study was the estimation of prevalence and molecular typing of Enterobius vermicularis among healthy children in north-eastern Poland. In 2013 – 2015, 296 individuals (aged 2 – 18 years) from 12 kindergartens, schools and orphanages were examined by the adhesive cellophane tape method. Data on socio-demographic status were collected using a questionnaire. Molecular analysis was performed using the DNA of adult female pinworms and primers targeting the region of cytochrome oxidase I gene. The overall prevalence of enterobiasis was 10.1 %. Enterobius vermicularis infection rates were 3.9 % in children living in families and 32.8 % among the orphans (OR=0.08; 95 % CI: 0.04 – 0.19; p<0.001). There were no associations between distribution of enterobiasis and gender, pets possession and the season of examination. In 43.3 % of the infected children enterobiasis was asymptomatic. Based on a molecular marker three different haplotypes of pinworm were identified. All sequences clustered within type B, together with human E. vermicularis isolates from Denmark, Germany, Greece, and Japan. This paper provides complementary data on the occurrence and intraspecific variability of E. vermicularis in human population in Europe.
O. P. Kurnosova, M. V. Arisov and I. M. Odoyevskaya
. J ., 177(1): 18 – 25. DOI: 10.1016/j. tvjl.2007.09.022
U mur , S., M eral , Y, B olukbas , C., G urler , A., A cici , M. (2017): First clinical Strongyloides stercoralis case in a dog in Turkey. Turk. J. Vet. Anim. Sci ., 4(1): 312 – 315. DOI: 10.3906/vet-1606-2
U pjohn , M., C obb , C., M onger , J.,G eurden , T., C laerebout , E., F ox , M. (2010): Prevalence, moleculartyping and risk factor analysis for Giardia duodenalis infections in dogs in a central London rescue shelter. Vet. Parasitol ., 172(3 – 4): 341 – 346. DOI: 10.1016/j. vetpar