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Collection of Listeria monocytogenes Isolates from Milk, Dairy Products and Food Processing Environments in Slovakia for the Purposes of European Molecular Database

., 2013: ECDC starts pilot phase for collection of molecular typing data. Euro Surveill. , 18, pii: 20357. 11. Véghová, A., Koreňová, J., Minarovičová, J., Drahovská, H., Siekel, P., Kaclíková, E., 2015: Isolation and characterization of Listeria monocytogenes from the environment of three ewes’ milk processing factories in Slovakia. Journal of Food and Nutrition Research , 54, 252—259.

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Application of Genomics in Clinical Oncology

Application of Genomics in Clinical Oncology

Genomics is a comprehensive study of the whole genome, genetic products, and their interactions. Human genome project has identified around 25,000-30,000 genes, and prevailing presence in tumor pathogenesis, high number of mutations, epigenetic changes, and other gene disorders have been identified. Microarrays technology is used for the analysis of these changes. Postgenome age has begun, and the initial results ensure the improvement of molecular tumor diagnostics and the making of a new taxonomic tumor classification, as well as the improvement, optimization and individualization of anti-tumor therapy. First genomic classifications have been made of leukemias, non-Hodgkin lymphoma, and many solid tumors. For example, 4 molecular types of breast carcinoma, three types of diffuse B cell lymphoma, two types of chromophobic renal carcinoma have been identified. Also, gene structures for favorable and unfavorable outcome in leukemia, breast cancer, prostate, bronchi, and other tumors have been identified. It is absolutely possible to diagnose the primary outcome of tumors with which standard tumor position may not be proved using standard diagnostic tools. Pharmacogenomic profiles have ensured better definition of interindividual differences during therapy using antineoplastic drugs and the decrease of their toxicity, as well as individual treatment approach and patient selection with which favorable clinical outcome is expected. Pharmacogenomics has impacted the accelerated development of target drugs, which have showed to be useful in practice. New genomic markers mtDNA, meDNA, and miRNA have been identified, which, with great certainty, help the detection and diagnostics of carcinoma. In the future, functional genomics in clinical oncology provides to gain knowledge about tumor pathogenesis; it will improve diagnostics and prognosis, and open up new therapeutic options.

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The Prevalence and Assemblages of Giardia Duodenalis in Dogs: A Systematic Review in Europe

Determination of Giardia in Dogs from South-Eastern Europe . Dissertation, LMU München, Faculty of Veterinary Medicine, 111 pp. 48. Sommer, M. F., Pietschc, M., Kaspara, A., Beelitza, P., 2018: Giardia in a selected population of dogs and cats in Germany — diagnostics, coinfections and assemblages. Vet. Parasitol. , 249, 49—56. DOI: 10.1016/j.vetpar.2017.11.006. 49. Stojecki, K., Sroka, J., Cacciò, S. M., Cencek, T., Dutkiewicz, J., Kusyk, D., 2015: Prevalence and molecular typing of Giardia duodenalis in wildlife from eastern Poland. Folia Parasitol. , 62

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Expanding PVL positive MRSA healthcare-associated infections outbreak in a newborns unit, caused by hospital hygiene shortcomings

spa type MRSA strains evolving in a Romanian newborns unit in 2010. Rom Rev Lab Med. 2011 Jun;Suppl.19 (2/4):S81-2. 64. Franco A, Hasman H, Iurescia M, Lorenzetti R, Stegger M, Pantosti A, et al. Molecular characterization of spa type t127, sequence type 1 methicillin-resistant Staphylococcus aureus from pigs. J Antimicrob Chemother. 2011 Jun;66(6):1231-5. DOI: 10.1093/jac/dkr115 65. Monecke S, Muller E, Dorneanu OS, Vremeră T, Ehricht R. Molecular Typing of MRSA and of Clinical Staphylococcus aureus Isolates from Iasi, Romania. PLoS ONE. 2014 May;9(5):e

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ERIC-based differentiation and antimicrobial susceptibility of Yersinia enterocolitica O:9 isolated from animals serologically positive and negative for brucellosis

primers. PCR Meth Appl 1993, 3, 85-94. 4. Falcao J.P., Falcao D.P., Pitondo-Silva A., Malaspina A.C., Brocchi M.: Molecular typing and virulence markers of Yersinia enterocolitica strains from human, animal and food origins isolated between 1968 and 2000 in Brazil. J Med Microbiol 2006, 55, 1539-1548. 5. Garin-Bastuji B., Hummel N., Gerbier G., Cau C., Pouillot R., Da Costa M., Fontaine J.J.: Nonspecific serological reactions in the diagnosis of bovine brucellosis: experimental oral infection of cattle with repeated doses of Yersinia

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Characterisation of Staphylococcus aureus and Staphylococcus aureus – like strains isolated from table eggs

.H., Byrne S.K., Zhang J.L., Chow A.W.: Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphism. J Clin Microbiol 1992, 30, 1642-1645. 11. Graham J.C., Murphy O.M., Stewart D., Kearns A.M., Galloway A., Freeman R.: Comparison of PCR detection of mecA with methicillin and oxacillin disc susceptibility testing in coagulase-negative staphylococci. J Antimicrob Chemother 2000, 45, 111-113. 12. Hussain Z., Stoakes L., Lannigan R., Longo S., Nancekivell B.: Evaluation of screening and commercial methods for detection

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Genetic diversity of Campylobacter jejuni isolated from the poultry food chain

-borne Campylobacter jejuni isolates in California. J Clin Microbiol 2013, 51, 195–201. 12. Kaakoush N.O., Castaño-Rodriguez N., Mitchell H.M., Man S.M.: Global epidemiology of Campylobacter infection. Clin Microbiol Rev 2015, 28, 687–720. 13. Lévesque S., Fournier E., Carrier N., Frost E., Arbeit R.D., Michaud S.: Campylobacteriosis in urban versus rural areas: A case-case study integrated with molecular typing to validate risk factors and to attribute sources of infection. PLoS ONE 2013, 8, e83731. 14. Müllner P., Spencer S.E.F., Wilson D.J., Jones G., Noble

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Multiplex real-time PCRs for detection of Salmonella, Listeria monocytogenes, and verotoxigenic Escherichia coli in carcasses of slaughtered animals

References 1. Alarcón B., García-Cañas V., Cifuentes A., González R., Aznar R.: Simultaneous and sensitive detection of three foodborne pathogens by multiplex PCR, capillary gel electrophoresis, and laser-induced fluorescence. J Agric Food Chem 2004, 52, 7180–7186. 2. Beutin L., Miko A., Krause G., Pries K., Haby S., Steege K., Albrecht N.: Identification of human-pathogenic strains of Shiga toxin producing Escherichia coli from food by a combination of serotyping and molecular typing of Shiga toxin genes. Appl Environ Microbiol 2007, 73, 4769

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Occurrence and characterisation of MRSA and extended-spectrum ß-lactamases producing Escherichia coli isolated from mastitic cows’ milk

Z., Wu J., Ali T., Li J., Lv Y., Han B.: Bovine mastitis Staphylococcus aureus: Antibiotic susceptibility profile, resistant genes and molecular typing of methicillinresistant and methicillin-sensitive strains in China. Infect Genet Evol 2015, 31, 9-16. 29. Wasiński B., Różańska H., Osek J.: Occurrence of extended spectrum ß-lactamase and AmpC-producing Escherichia coli in meat samples. Bull Vet Inst Pulawy 2013, 57, 513-517. 30. Watson E., Jeckel S., Snow L., Stubbs R., Teale Ch., Wearing H., Horton R., Toszeghy M., Tearne O

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Characterisation of newly emerged isolates of classical swine fever virus in China, 2014–2015

References 1. Bhaskar N., Ravishankar C., Rajasekhar R., Sumod K., Sumithra T.G., John K., Mini M., Ravindran R., Shaji S., Aishwarya J.: Molecular typing and phylogenetic analysis of classical swine fever virus isolates from Kerala, India. Virus Dis 2015, 26, 260–266. 2. Chen N., Hu H., Zhang Z., Shuai J., Jiang L., Fang W.: Genetic diversity of the envelope glycoprotein E2 of classical swine fever virus: recent isolates branched away from historical and vaccine strains. Vet Microbiol 2008, 127, 286–299. 3. Chen N., Li D., Yuan X., Li X., Hu H

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