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Collection of Listeria monocytogenes Isolates from Milk, Dairy Products and Food Processing Environments in Slovakia for the Purposes of European Molecular Database

., 2013: ECDC starts pilot phase for collection of molecular typing data. Euro Surveill. , 18, pii: 20357. 11. Véghová, A., Koreňová, J., Minarovičová, J., Drahovská, H., Siekel, P., Kaclíková, E., 2015: Isolation and characterization of Listeria monocytogenes from the environment of three ewes’ milk processing factories in Slovakia. Journal of Food and Nutrition Research , 54, 252—259.

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Molecular typing of bacteria for epidemiological surveillance and outbreak investigation / Molekulare Typisierung von Bakterien für die epidemiologische Überwachung und Ausbruchsabklärung

infectious diseases are a leading cause of morbidity worldwide ( Rowell et al., 2012 ; Palm et al., 2014). The aim of this outline is to review the value of strain typing in public health and in the food industry. Selected examples are used to give an overview on current molecular typing tools, discussing the potential as well as the shortcomings of diverse techniques in reference to our own work and to present an outlook on upcoming technologies based on WGS. 2 Identification of microorganisms Identification denotes the assignment of a microorganism into a

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Application of Genomics in Clinical Oncology

Application of Genomics in Clinical Oncology

Genomics is a comprehensive study of the whole genome, genetic products, and their interactions. Human genome project has identified around 25,000-30,000 genes, and prevailing presence in tumor pathogenesis, high number of mutations, epigenetic changes, and other gene disorders have been identified. Microarrays technology is used for the analysis of these changes. Postgenome age has begun, and the initial results ensure the improvement of molecular tumor diagnostics and the making of a new taxonomic tumor classification, as well as the improvement, optimization and individualization of anti-tumor therapy. First genomic classifications have been made of leukemias, non-Hodgkin lymphoma, and many solid tumors. For example, 4 molecular types of breast carcinoma, three types of diffuse B cell lymphoma, two types of chromophobic renal carcinoma have been identified. Also, gene structures for favorable and unfavorable outcome in leukemia, breast cancer, prostate, bronchi, and other tumors have been identified. It is absolutely possible to diagnose the primary outcome of tumors with which standard tumor position may not be proved using standard diagnostic tools. Pharmacogenomic profiles have ensured better definition of interindividual differences during therapy using antineoplastic drugs and the decrease of their toxicity, as well as individual treatment approach and patient selection with which favorable clinical outcome is expected. Pharmacogenomics has impacted the accelerated development of target drugs, which have showed to be useful in practice. New genomic markers mtDNA, meDNA, and miRNA have been identified, which, with great certainty, help the detection and diagnostics of carcinoma. In the future, functional genomics in clinical oncology provides to gain knowledge about tumor pathogenesis; it will improve diagnostics and prognosis, and open up new therapeutic options.

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Pulsed-Field Gel Electrophoresis Used for Typing of Extended-Spectrum-β-Lactamases- Producing Escherichia coli Isolated from Infant ҆S Respiratory and Digestive System

.3.59 PMid:16602980 20. Caprioli, A., Maugliani, A., Michelacci, V., Morabito, S. (2014). Molecular typing of Verocytotoxin -producing E. coli (VTEC) strains isolated from food, feed and animals : state of play and standard operating procedures for pulsed field gel electrophoresis (PFGE) typing, profiles interpretation and curation. EFSA journal EN704, 55. 21. Barrett, T.J., Gerner-Smidt, P., Swaminathan, B. (2006). Interpretation of pulsed-field gel electrophoresis patterns in foodborne disease investigations and surveillance. Foodborne Pathog Dis. 3(1): 20

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The Prevalence and Assemblages of Giardia Duodenalis in Dogs: A Systematic Review in Europe

Determination of Giardia in Dogs from South-Eastern Europe . Dissertation, LMU München, Faculty of Veterinary Medicine, 111 pp. 48. Sommer, M. F., Pietschc, M., Kaspara, A., Beelitza, P., 2018: Giardia in a selected population of dogs and cats in Germany — diagnostics, coinfections and assemblages. Vet. Parasitol. , 249, 49—56. DOI: 10.1016/j.vetpar.2017.11.006. 49. Stojecki, K., Sroka, J., Cacciò, S. M., Cencek, T., Dutkiewicz, J., Kusyk, D., 2015: Prevalence and molecular typing of Giardia duodenalis in wildlife from eastern Poland. Folia Parasitol. , 62

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Expanding PVL positive MRSA healthcare-associated infections outbreak in a newborns unit, caused by hospital hygiene shortcomings

spa type MRSA strains evolving in a Romanian newborns unit in 2010. Rom Rev Lab Med. 2011 Jun;Suppl.19 (2/4):S81-2. 64. Franco A, Hasman H, Iurescia M, Lorenzetti R, Stegger M, Pantosti A, et al. Molecular characterization of spa type t127, sequence type 1 methicillin-resistant Staphylococcus aureus from pigs. J Antimicrob Chemother. 2011 Jun;66(6):1231-5. DOI: 10.1093/jac/dkr115 65. Monecke S, Muller E, Dorneanu OS, Vremeră T, Ehricht R. Molecular Typing of MRSA and of Clinical Staphylococcus aureus Isolates from Iasi, Romania. PLoS ONE. 2014 May;9(5):e

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S-Genotype Diversity in Wild Cherry Populations in the Czech Republic

. Annual Review of Plant Biology, 56, 467-489. doi: 10.1146/annurev.arplant.56.032604.144249. Tao R, Yamane H, Sugiura A, Murayama H, Sassa H, Mori H (1999): Molecular typing of S-alleles through identification, characterization and cDNA cloning for S-RNases in sweet cherry. Journal of the American Society for Horticultural Science, 124, 224-233. Tobutt KR, Sonneveld T, Bekefi Z, Boskovic R (2004): Cherry (in)compatibility genotypes - an updated cultivar table. Acta Hortica, 663, 667-672. Ushijima K, Sassa H, Tao R, Yamane

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Enterobiasis epidemiology and molecular characterization of Enterobius vermicularis in healthy children in north-eastern Poland

Summary

Enterobiasis is a human intestinal parasitic disease caused by human pinworm, Enterobius vermicularis. Despite being the most prevalent nematode infection in Europe and North America, predominantly among in school aged children, the data concerning infection rate and knowledge of genetic variability of pinworms are incomplete. The aim of the study was the estimation of prevalence and molecular typing of Enterobius vermicularis among healthy children in north-eastern Poland. In 2013 – 2015, 296 individuals (aged 2 – 18 years) from 12 kindergartens, schools and orphanages were examined by the adhesive cellophane tape method. Data on socio-demographic status were collected using a questionnaire. Molecular analysis was performed using the DNA of adult female pinworms and primers targeting the region of cytochrome oxidase I gene. The overall prevalence of enterobiasis was 10.1 %. Enterobius vermicularis infection rates were 3.9 % in children living in families and 32.8 % among the orphans (OR=0.08; 95 % CI: 0.04 – 0.19; p<0.001). There were no associations between distribution of enterobiasis and gender, pets possession and the season of examination. In 43.3 % of the infected children enterobiasis was asymptomatic. Based on a molecular marker three different haplotypes of pinworm were identified. All sequences clustered within type B, together with human E. vermicularis isolates from Denmark, Germany, Greece, and Japan. This paper provides complementary data on the occurrence and intraspecific variability of E. vermicularis in human population in Europe.

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Occurrence of Cryptosporidium Spp. and Giardia Duodenalis in Red Foxes (Vulpes Vulpes) in Bosnia and Herzegovina

:21744018 11. Hamnes, I. S, Gjerde, B. K., Robertson, L. J. (2007b). A longitudinal study on the occurrence of Cryptosporidium and Giardia in dogs during their first year of life. Acta Vet Scand. 49, 22. http://dx.doi.org/10.1186/1751-0147-49-22 PMid:17848186; PMCid:PMC2040143 12. Beck, R., Sprong, H., Bata, I., Lucinger, S., Pozio, E., Cacció, S. M. (2011b). Prevalence and molecular typing of Giardia spp. in captive mammals at the ZOO of Zagreb, Croatia. Vet Parasitol. 175, 40-46. http://dx.doi.org/10.1016/j.vetpar.2010.09.026 PMid

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The Effects of Different Storage Temperatures and Time on The Survival of Cpe (+) Clostridium Perfringens Type A in Çiğ Köfte (A Traditional Turkish Raw Meat Product)

M.E. (2014). The prevalence and molecular typing of Clostridium petfiingetxs in ground beef and sheep meats. J. Verbrauch. Lebensm., 9: 121-128. Jay J.M, Loessner M.J., Golden D.A. (2005). Editors. Modem Food Microbiology. Springer Science, New York, 7th ed., 790 pp. Junej a VK, Marks H., Thippareddi H. (2008). Predictive model for growth of Clostridium perfiingetxs during cooling of cooked uncured beef. Food MicrobioL, 25: 42-55. Kiic B. (2009). Current trends in traditional Turkish meat products and cuisine. LWT

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