Amina H Hassab, Dalia A Nafea, Rania S Swelem and Basma M Ghazal
were performed using Becton Dickinson, FACSCalibur flow cytometer equipped with Cell Quest software (USA) [ 14 , 15 , 16 ].
The detailed characterization of hematopoietic cells was done by analyzing the expression of a given set of antigens in a cell population. The applied panels of monoclonal antibodies (McAbs) for diagnosis of acute leukemia were as follows: primary panel: CD2 – phycoerythrin (PE); CD5 – PE; CD7 – fluorescein isothiocyanate (FITC); CD10 – FITC; CD19 – R-phycoerythrin (RPE); CD14 – FITC; CD13 – PE; CD33 – PE
Agnieszka Pluta, Tadeusz Robak, Kamil Brzozowski, Barbara Cebula-Obrzut, Agata Majchrzak, Piotr Pluta, Anna Szmigielska-Kapłon, Olga Grzybowska-Izydorczyk, Magdalena Czemerska, Piotr Stelmach, Piotr Smolewski and Agnieszka Wierzbowska
treatment failure. Disease-free survival (DFS) was calculated from the first day of CR until documentation of a relapse [ 3 ]. OS was calculated from the time of diagnosis until death [ 3 ].
Blood and BM sampling
Venous blood or BM samples were collected at the time of diagnosis into pyrogen-free ethylenediaminetetraacetic acid (EDTA) tubes. Immunophenotyping of leukemic cells was performed routinely in the whole peripheral blood or BM using flow cytometry: the “lysed-not washed” method. A routine panel of monoclonal antibodies HLA-DR, CD34, CD45
Cristiane da Silva Rodrigues de Araújo, Mirna Maira Bezerra Calazan do Carmo, Claudia de Alvarenga Maximo and Sílvia Maia Farias de Carvalho
, hematological findings, increased LDH value, detection of HTLV-I antibodies and immunophenotyping analyses. Four subtypes were used to classify the patients according to the classical criteria established by Shimoyama et al [ 39 ].
Morphology and Immunophenotyping
Bone-marrow aspirate and/or Peripheral-blood were examined in all cases according to Catovsky et al (1984). Immunophenotyping analyses were performed routinely using a clean peripheral blood mononuclear cell (PBMC) from the patients, by flow cytometry using a panel of fluorochrome-conjugated monoclonal
detected, with elevated IgM (6130 mg/dL) and relatively normal IgG and IgA levels (803 mg/dL and 136 mg/dL, respectively). BM examination revealed hypercellular marrow (nuclear cell count was 24.0 × l0 4 /mm 3 ) with 0/mm 3 megakaryocytes. Lymphoid cells were increased (80.8%) and were diffusely infiltrated into residual normal BM cells. The cells were small-to-medium-sized ones, with a high nucleus-to-cytoplasm (N/C) ratio and round-to-oval-shaped nuclei. An immunohistochemical study and flow cytometric (FCM) analysis demonstrated that the tumor cells were positive for
Henu Kumar Verma, Saikrishna Lakkakula and Bhaskar V.K.S. Lakkakula
Asia, Indian subcontinent, and the Far East. Highest incidences of beta-thalassemia are found in populations of Cyprus (14%), Sardinia (12%), and Southeast Asia [ 5 ]. Alpha-thalassemia is more common in sub-Saharan Africa, the Mediterranean Basin, the Middle East, South Asia, and Southeast Asia [ 6 , 7 , 8 , 9 ].
In SCA, the deformed RBCs tend to get stuck in narrow blood capillaries and block the blood flow. Patients experience vaso-occlusive crisis (VOC) in their joints and bones, along with severe pain, which causes multiple organ damage ( Figure 2 ) in SCD
Conflicts of interest
Konflikt interesu/Conflict of interest: Nie występuje.
Treści przedstawione w artykule są zgodne z zasadami Deklaracji Helsińskiej, dyrektywami EU oraz ujednoliconymi wymaganiami dla czasopism biomedycznych.
 Wiczling P, Krzyzanski W. Flow cytometric assessment of homeostatic aging of reticulocytes in rats. Exp Hematol 2008;36(2):119–27. doi: 10.1016/j.exphem.2007.09.002. 10.1016/j.exphem.2007.09.002
;93:3451–56. 10233897 Hugel B Socie G Vu T et al Elevated levels of circulating procoagulant microparticles in patients with paroxysmal nocturnal hemoglobinuria and aplastic anemia Blood 1999 93 3451 56
 Vidal C, Spaulding C, Picard F, et al. Flow cytometry detection of platelet procoagulation activity and microparticles in patients with unstable angina treated by percutaneous coronary angioplasty and stent implantation. Thromb Haemost 2001;86:784–90. 10.1055/s-0037-1616132 11583308 Vidal C Spaulding C Picard F et al Flow cytometry
Monika Adamska, Anna Komosa, Tatiana Mularek, Joanna Rupa-Matysek and Lidia Gil
:3. Bone marrow biopsy in AL amyloidosis may reveal an increased percentage of plasma cells that may appear morphologically normal, with a median percentage of <10% [ 25 ]. Immunoperoxidase staining or flow cytometric analysis of specimens of the involved bone marrow can demonstrate clonal excess of plasma clone cells. Bone marrow biopsy is 50% sensitive for detecting deposits of amyloid, but, performed with fat pad aspirate, can be up to 85% sensitive [ 26 ].
Biomarker-based staging systems are crucial for patient stratification in clinical
Sebastian Giebel, Sylwia Oborska, Joanna Romejko-Jarosinska, Jarosław Dybko, Joanna Mańko, Joanna Sawczuk-Chabin, Agata Szymańska, Wojciech Legieć, Anna Czyż, Magdalena Maruszak, Maria Saduś-Wojciechowska, Joanna Drozd-Sokołowska, Paweł Steckiewicz, Anna Ejduk, Ewa Paszkiewicz-Kozik, Tomasz Ogórka, Michał Osowiecki, Łukasz Targoński and Michał Taszner
the peripheral blood was < 10/μL 4-6 days after the onset of G-CSF administration as monotherapy or within 20 days after the onset of administration of chemotherapy in combination with G-CSF.
For the decision to start leukaphereses the number of circulating CD34 + cells was first evaluated on the second day of neutrophil recovery > 1 × 10 9 /L in patients who experienced grade 3 or 4 neutropenia or, in the remaining patients, on the first day with increase of neutrophil count. The analysis was done using flow cytometry, according to local protocols
Samia Abd El-Moneim Ebied, Nadia Aly Sadek, Nadia El-Sayed Zaki, Samir Ali Abd El- Kaream and Heba Khafagui Ahmed El Kashif
peripheral blood. Evaluation for morphology, flow cytometry, immunophenotyping and cytogenetic testing is valuable both for confirming the diagnosis and risk stratification. Lumbar puncture with CSF analysis is standard of care at the time of diagnosis to evaluate for CNS involvement. If the CNS is involved, brain MRI should be performed. Other evaluation includes complete blood count with differential and smear to evaluate the other hematopoietic cell lines, coagulation profiles and serum chemistries. Baseline uric acid, calcium, phosphate and lactate dehydrogenase should