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). Our data identified higher levels of SUA in the NASH group when compared to steatosis at a baseline, which confirms results from cited studies.
Interestingly, a reduction of SUA levels was detected after our drug intervention. This observation may be explained by PTX effect on minimizing liver damage while reducing SUA levels as suggested in lately published research ( 38 ). Furthermore, PTX has been indicated to restore depressed cardiac output and improve hepatic perfusion and intestinal blood flow in case of hemorrhage and resuscitation ( 39 ). We hypothesized
Sandra Vladimirov, Tamara Gojkovic, Aleksandra Zeljkovic, Zorana Jelic-Ivanovic and Vesna Spasojevic-Kalimanovska
Instrumental conditions for HPLC-MS/MS NCSs’ analyses
All the analyses were performed under the same chromatographic and MS/MS conditions. Separation of the sterols and oxysterols was achieved by using Porochell 120 EC column (150×4.6 mm×2.7 μm) by Agilent Technologies (USA). Chromatographic conditions included isocratic elution with constant mobile phase flow of 0.6 mL/min and column temperature of 30 °C. The mobile phase was composed of acetonitrile: methanol: water with 0.1% formic acid (80: 18: 2, v/v). Sample injection volume was 10 μL, and all the non
Katarzyna Stefańska, Ievgenia Kocherova, Sandra Knap, Magdalena Kulus, Piotr Celichowski and Michal Jeseta
of ciliated OECs is to support the transport of gametes and embryos through the ovary. They generate the movement of the oviductal fluid, with and the direction of the flow depending on the species; in pigs it is towards the ovary [ 1 ]. Oviductal fluid contains glucose, lactate, pyruvate, amino acids, embryotropic and growth factors and other molecules essential for both gamete and embryo survival. However, the composition of oviductal fluid and secretory activity of the oviductal epithelial cells changes during the estrous cycle in response to hormone levels [ 2
Ievgeniia Kocherova, Maciej Brązert, Patrycja Sujka-Kordowska, Aneta Konwerska, Magdalena Kulus, Błażej Chermuła, Piotr Celichowski, Hanna Piotrowska-Kempisty, Paweł Antosik, Dorota Bukowska, Małgorzata Bruska, Leszek Pawelczyk, Maciej Zabel, Michał Nowicki, Bartosz Kempisty and Michal Jeseta
. Ovulation is considered to be a major stressor for surrounding cells, causing trauma-induced inflammation and unbalanced ROS levels, leading to local oxidative stress [ 11 , 12 ].
Animal oviducts and human Fallopian tubes represent a part of the female reproductive tract subjected to periodic exposure to free radicals. Whereas ROS contained in follicular fluid mainly affect the fimbriae, the backward flow of menstrual debris through the tubes, called menstrual reflux, may influence the oxygen tension along the entire uterine tube lumen. Exposure to free radicals
Mikołaj Lorenz, Paul Mozdziak, Bartosz Kempisty and Marta Dyszkiewicz-Konwińska
based on the Percoll density gradient centrifugation. However, cells that were selected as TSCs based on surface markers accounted for about 1% of the population [ 13 ]. The second attempted method has selected a similar number of cells, but is based on flow cytometry that isolates a side population of TSCs due to faster pumping of Hoechst 33342 dye from the cytoplasm. The third method is also based on Hoechst staining following trypsin digestions [ 13 ]. Only 0.14% were isolated in Takao’s work [ 14 ]. Further research is being carried out on the ability to
Sandra Vladimirov, Aleksandra Zeljkovic, Tamara Gojkovic, Milica Miljkovic, Aleksandra Stefanovic, Dejan Zeljkovic, Bratislav Trifunovic and Vesna Spasojevic-Kalimanovska
extracts were collected in the same vial and evaporated to dryness. In the final step, the extract was reconstituted in 20 μL of methanol and injected into the LC-MS/MS instrument. Chromatographic separation of 25(OH)D3 and 24,25(OH) 2 D3 and corresponding internal standards was achieved in under 20 min using Kinetex F5 (150×4.6 mm, 2.6 μm, 100Å) LC column with corresponding guard column and isocratic mobile phase methanol: water (85: 15, v: v). Mobile phase flow was 0.3 mL/min and column temperature 20 °C. Quantification was done using multiple-reaction-monitoring (MRM
Lisa Moncrieff, Paul Mozdziak, Michal Jeseta, Marie Machatkova, Wiesława Kranc and Bartosz Kempisty
these patients function abnormally and lower levels of sex hormones like oestrogen are synthesised. As a consequence, ovulation occurs infrequently. To restore fertility, VSELS still have potential to be used after cancer treatment as they are not eliminated by chemotherapy [ 7 , 49 ]. They survive toxic insults due to their quiescent ability, however, this characteristic has the downside of making them not divide readily in culture [ 13 ].
Stem cells can be directed to the germ cell lineage [ 42 , 50 , 51 ]. Specifically, flow cytometry employing on germline