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Biochemical Effects of Low-Flow Anesthesia with Inhalation agents in Patients undergoing Laparoscopic Surgery

References Sivaci R, Orman A, Yilmazer M, Yilmaz S, Ellidokuz H, Polat C. The effect of low-flow sevoflurane and desflurane on pulmonary mechanics during laparoscopic surgery. J Laparoendoscopic & Advanced Surgical Techniuges A 2005; 15(2): 125-9. Strum EM, Szenohradszki J, Kaufman WA, Anthone GJ, Manz IL, Lumb PD. Emergence and Recovery Characteristics of Desflurane Versus Sevoflurane in Morbidly Obese Adult Surgical Patients: A Prospective, Randomized study. Anesthesia & Analgesia 2004; 99: 1848

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Flow Cytometric Assay of Respiratory Burst Capacity in Peripheral Blood Monocytes of Gaucher Type 1 Patients

. Impaired microbicidal capacity of mononuclear phagocytes from patients with type I Gaucher disease: partial correction by enzyme replacement therapy. Blood 1995; 86(12): 4645-9. 14. Lehmann AK, Sornes S, Halstensen A. Phagocytosis: measurement by flow cytometry. J Immunol Methods 2000; 243(1-2): 229-42. 15. Rothe G, Oser A, Valet G. Dihydrorhodamine 123: a new flow cytometric indicator for respiratory burst activity in neutrophil granulocytes. Naturwissenschaften 1988; 75(7): 354-5. 16. Lehmann AK, Halstensen A, Bassoe CF

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Transcriptional Activity of Gene Encoding Subunits R1 and R2 of Interferon Gamma Receptor in Peripheral Blood Mononuclear Cells in Patients with Slow Coronary Flow

References 1. Tambe A, Demany M, Zimmerman HA, Mascarenhas E. Angina pectoris and slow flow velocity of dye in coronary arteries—a new angiographic finding. Am Heart J 1972; 84(1): 66–71. 2. Beltrame JF, Limaye SB, Horowitz JD. The coronary slow flow phenomenon—a new coronary microvascular disorder. Cardiology 2001; 97(4): 197–202. 3. Mangieri E, Macchiarelli G, Ciavolella M, Barillà F, Avella A, Martinotti A, et al. Slow coronary flow: clinical and histopathological features in patients with otherwise normal epicardial coronary arteries. Cathet

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Pentoxifylline with metformin treatment improves biochemical parameters in patients with nonalcoholic steatohepatitis

). Our data identified higher levels of SUA in the NASH group when compared to steatosis at a baseline, which confirms results from cited studies. Interestingly, a reduction of SUA levels was detected after our drug intervention. This observation may be explained by PTX effect on minimizing liver damage while reducing SUA levels as suggested in lately published research ( 38 ). Furthermore, PTX has been indicated to restore depressed cardiac output and improve hepatic perfusion and intestinal blood flow in case of hemorrhage and resuscitation ( 39 ). We hypothesized

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Determination of non-cholesterol sterols in serum and HDL fraction by LC/MS-ms: Significance of matrix-related interferences

.5. Instrumental conditions for HPLC-MS/MS NCSs’ analyses All the analyses were performed under the same chromatographic and MS/MS conditions. Separation of the sterols and oxysterols was achieved by using Porochell 120 EC column (150×4.6 mm×2.7 μm) by Agilent Technologies (USA). Chromatographic conditions included isocratic elution with constant mobile phase flow of 0.6 mL/min and column temperature of 30 °C. The mobile phase was composed of acetonitrile: methanol: water with 0.1% formic acid (80: 18: 2, v/v). Sample injection volume was 10 μL, and all the non

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The genes regulating maintenance of cellular protein location are differentially expressed in porcine epithelial oviductal cells during longterm in vitro cultivation

of ciliated OECs is to support the transport of gametes and embryos through the ovary. They generate the movement of the oviductal fluid, with and the direction of the flow depending on the species; in pigs it is towards the ovary [ 1 ]. Oviductal fluid contains glucose, lactate, pyruvate, amino acids, embryotropic and growth factors and other molecules essential for both gamete and embryo survival. However, the composition of oviductal fluid and secretory activity of the oviductal epithelial cells changes during the estrous cycle in response to hormone levels [ 2

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Genes regulating biochemical pathways of oxygen metabolism in porcine oviductal epithelial cells during long-term IVC

. Ovulation is considered to be a major stressor for surrounding cells, causing trauma-induced inflammation and unbalanced ROS levels, leading to local oxidative stress [ 11 , 12 ]. Animal oviducts and human Fallopian tubes represent a part of the female reproductive tract subjected to periodic exposure to free radicals. Whereas ROS contained in follicular fluid mainly affect the fimbriae, the backward flow of menstrual debris through the tubes, called menstrual reflux, may influence the oxygen tension along the entire uterine tube lumen. Exposure to free radicals

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Application potential and plasticity of human stem cells

based on the Percoll density gradient centrifugation. However, cells that were selected as TSCs based on surface markers accounted for about 1% of the population [ 13 ]. The second attempted method has selected a similar number of cells, but is based on flow cytometry that isolates a side population of TSCs due to faster pumping of Hoechst 33342 dye from the cytoplasm. The third method is also based on Hoechst staining following trypsin digestions [ 13 ]. Only 0.14% were isolated in Takao’s work [ 14 ]. Further research is being carried out on the ability to

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Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer

extracts were collected in the same vial and evaporated to dryness. In the final step, the extract was reconstituted in 20 μL of methanol and injected into the LC-MS/MS instrument. Chromatographic separation of 25(OH)D3 and 24,25(OH) 2 D3 and corresponding internal standards was achieved in under 20 min using Kinetex F5 (150×4.6 mm, 2.6 μm, 100Å) LC column with corresponding guard column and isocratic mobile phase methanol: water (85: 15, v: v). Mobile phase flow was 0.3 mL/min and column temperature 20 °C. Quantification was done using multiple-reaction-monitoring (MRM

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Ovarian follicular cells - living in the shadow of stemness cellular competence

these patients function abnormally and lower levels of sex hormones like oestrogen are synthesised. As a consequence, ovulation occurs infrequently. To restore fertility, VSELS still have potential to be used after cancer treatment as they are not eliminated by chemotherapy [ 7 , 49 ]. They survive toxic insults due to their quiescent ability, however, this characteristic has the downside of making them not divide readily in culture [ 13 ]. Stem cells can be directed to the germ cell lineage [ 42 , 50 , 51 ]. Specifically, flow cytometry employing on germline

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