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Intravaginal Administration of Sildenafil Citrate Increases Blood Flow in the Bovine Uterus

References 1. Acosta T.J., Hayashi K.G, Ohtani M, Miyamoto A.: Local changes in blood flow within the preovulatory follicle wall and early corpus luteum in cows. Reproduction 2003, 125, 759-767. 2. Alatas E, Yaqci A.B.: The effect of sildenafil citrate on uterine and clitoral arterial blood flow in postmenopausal women. Med Gen Med 2004, 6, 51. 3. Bollwein H, Baumgartner U, Stolla R: Transrectal Doppler sonography of uterine blood flow in cows during pregnancy Theriogenology 2002, 57, 2053-2061. 4. Bollwein H, Kolberg B, Stolla R: The effect of

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Biochemical Effects of Low-Flow Anesthesia with Inhalation agents in Patients undergoing Laparoscopic Surgery

References Sivaci R, Orman A, Yilmazer M, Yilmaz S, Ellidokuz H, Polat C. The effect of low-flow sevoflurane and desflurane on pulmonary mechanics during laparoscopic surgery. J Laparoendoscopic & Advanced Surgical Techniuges A 2005; 15(2): 125-9. Strum EM, Szenohradszki J, Kaufman WA, Anthone GJ, Manz IL, Lumb PD. Emergence and Recovery Characteristics of Desflurane Versus Sevoflurane in Morbidly Obese Adult Surgical Patients: A Prospective, Randomized study. Anesthesia & Analgesia 2004; 99: 1848

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Flow Cytometric Assay of Respiratory Burst Capacity in Peripheral Blood Monocytes of Gaucher Type 1 Patients

. Impaired microbicidal capacity of mononuclear phagocytes from patients with type I Gaucher disease: partial correction by enzyme replacement therapy. Blood 1995; 86(12): 4645-9. 14. Lehmann AK, Sornes S, Halstensen A. Phagocytosis: measurement by flow cytometry. J Immunol Methods 2000; 243(1-2): 229-42. 15. Rothe G, Oser A, Valet G. Dihydrorhodamine 123: a new flow cytometric indicator for respiratory burst activity in neutrophil granulocytes. Naturwissenschaften 1988; 75(7): 354-5. 16. Lehmann AK, Halstensen A, Bassoe CF

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Transcriptional Activity of Gene Encoding Subunits R1 and R2 of Interferon Gamma Receptor in Peripheral Blood Mononuclear Cells in Patients with Slow Coronary Flow

References 1. Tambe A, Demany M, Zimmerman HA, Mascarenhas E. Angina pectoris and slow flow velocity of dye in coronary arteries—a new angiographic finding. Am Heart J 1972; 84(1): 66–71. 2. Beltrame JF, Limaye SB, Horowitz JD. The coronary slow flow phenomenon—a new coronary microvascular disorder. Cardiology 2001; 97(4): 197–202. 3. Mangieri E, Macchiarelli G, Ciavolella M, Barillà F, Avella A, Martinotti A, et al. Slow coronary flow: clinical and histopathological features in patients with otherwise normal epicardial coronary arteries. Cathet

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Patterns of Pollen Flow and Genetic Differentiation Among Pollen Pools in Quercus salicina in a Warm Temperate Old–growth Evergreen Broad-leaved Forest

): Exclusion of paternity: the current state of the art. Am J Hum Genet 26: 477-488. CHASE, M. R., C. MOLLER, R. KESSELI and K. S. BAWA (1996): Distant gene flow in tropical trees. Nature 383: 398-399. DOW, B. D. and M. V. ASHLEY (1996): Microsatellite analysis of seed dispersal and parentage of saplings in bur oak, Quercus macrocarpa. Mol Ecol 5: 615-627. DOW, B. D. and M. V. ASHLEY (1998): High levels of gene flow in bur oak revealed by paternity analysis using microsatellites. Journal of Heredity 89: 62

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Gene flow and mating system of the tropical tree Sextonia rubra

References AUSTERLITZ, F. and P. E. SMOUSE (2001): Two-Generation Analysis of Pollen Flow Across a Landscape. II. Relation Between {{Phi}}ft, Pollen Dispersal and Interfemale Distance. Genetics 157: 851-857. AUSTERLITZ, F. and P. E. SMOUSE (2002): Two-Generation Analysis of Pollen Flow Across a Landscape. IV. Estimating the Dispersal Parameter. Genetics 161: 355-363. BAWA, K. S., S. H. BULLOCK, D. R. PERRY, R. E. COVILLE and M. H. GRAYUM (1985): Reproductive biology of tropical lowland rain forest trees. II

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A regression approach to estimate the relative roles of pollen- versus seed-mediated gene flow under an isolation by distance model

estimation of neighbourhood parameters for plant pop­ulations. Heredity 52(2): 273-283. Dainou K, Bizoux J, Doucet J, Mahy G, Hardy O J, Heuert M (2010) Forest refugia revisited: nSSRs and cpDNA sequences support historical isolation in a wide-spread African tree with high colonization capacity, Milicia excelsa (Moraceae). Mol Ecol 19: 4462-4477. Ducousso A, Michaud H, Lumaret R (1993) Reproduction and gene flow in the genus Quercus L. Ann Sci Forest 50

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Flow Cytometric Determination of the Expression of gp 51 Protein of Bovine Leukaemia Virus in Experimentally Infected Sheep


The study was performed on lambs experimentally infected with bovine leukaemia virus (BLV). The presence of BLV antibodies in sera of infected animals was detected by agar gel immunodiffusion test and ELISA. Proviral DNA was detected by PCR and nested PCR. Dual-colour flow cytometry analysis was performed with the use of specific monoclonal antibodies against lymphocyte CD markers and gp51 viral envelope protein, followed by incubation with fluorescent-labelled secondary antibodies conjugated with FITC or PE. Gp51 viral envelope protein was detected in tumours caused by BLV infection. The BLV infection resulted in depletion of CD4+ lymphocytes, increase in CD8+ lymphocytes, and decrease in CD4+ to CD8+ ratio in infected sheep. Proliferation of IgM+ CD19+ cells was also detected. These cells had an immature character without tendency to differentiate, and their vitality was prolonged. Flow cytometry enabled detection of gp51 expression in sheep blood lymphocytes at the early stages of the infection, before detection of serum antibodies using ELISA.

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Endothelial Dysfunction Markers in Low Cardiovascular Risk Individuals: Comparison of Males and Females

, Selwyn AP, Ganz P. Coronary vasomotor response to acetylcholine relates to risk factors for coronary artery disease. Circulation 1990; 81: 1–7. 9. Schächinger V, Britten MB, Elsner M, Walter DH, Scharrer I, Zeiher AM. A positive family history of premature coronary artery disease is associated with impaired endothelium-dependent coronary blood flow regulation. Circulation 1999; 100: 2–8. 10. Kern MJ, Bach RG, Mechem CJ, Caracciolo EA, Aguirre FV, Miller LW, Donohue TJ. Variations in normal coronary vasodilatory reserve stratified by artery, gender, heart

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Assessment of selected exercise-induced CD3+ cell subsets and cell death parameters among soccer players

period. PBMC isolation To obtain peripheral blood mononuclear cells, whole blood samples were centrifuged for 15 min at 2500× g , room temperature (RT). The buffy coat was transferred to Lymphocyte Separation Medium (density 1.077–1.080 g/mL at 20 °C) (Corning, Manassas, VA, USA) and centrifuged 40 min at 350×g, RT. The obtained PBMC fraction was washed using Stain buffer (BD Pharmingen™, San Jose, CA, USA) and used for further analyses. Flow cytometric analyses All flow cytometry analyses were performed using a BD Accuri™ C6 flow cytometer (Becton

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