Mojca Tuta, Nina Boc, Erik Brecelj, Mirko Omejc, Franc Anderluh, Ajra Secerov Ermenc, Ana Jeromen Peressutti, Irena Oblak, Bojan Krebs and Vaneja Velenik
or with the presence of EMVI or extramesorectal lymph nodes involvement on magnetic resonance imaging (MRI) were considered candidates for TNT. Later on two more indications for this treatment selection were added: N2 disease and the distance £ 1 mm of tumor or lymph nodes from mesorectal fascia (MRF).
The main objective of the present study is to evaluate efficiency and toxicity of TNT treatment in LARC with high risk factors for local or distant recurrence in Slovenia.
Patients and methods
This prospective observational study included
Ming-Jun Fan, Shu-Mei Liang, Peng-Juan He, Xing-Bo Zhao, Ming-Jiang Li and Feng Geng
surgery, radiotherapy, chemotherapy and hormone therapy for early-stage diseases. 5 However, therapeutic efficiency of above treatments was extremely limited, hence searching for potential therapeutic targets in EAC become urgent.
Tumor suppressive role of dual-specificity phosphatase 6 (DUSP6) in cancers is suggested to act through ERK/MAPK signaling pathway. Intracellular ERK/MAPK signaling pathway is involved in a variety of cancer transformation as well as their tumorigenesis such as breast cancer 10 , lung cancer 11 , ovarian cancer 12 , pancreatic cancer. 13
Jerneja Varl, Urska Ivanus, Ziva Pohar Marinsek, Tine Jerman, Anja Ostrbenk Valencak, Mario Poljak and Veronika Kloboves Prevodnik
intraepithelial neoplasia grade 2 and 3 Eur J Gynaecol Oncol 2012 33 261 4 PMID: 22873095
8 de Cremoux P, Coste J, Sastre-Garau X, Thioux M, Bouillac C, Labbe S, et al. Efficiency of the Hybrid Capture 2 HPV DNA test in cervical cancer screening. A study by the French Society of Clinical Cytology. Am J Clin Pathol 2003; 120: 492-9. doi: 10.1309/XFUC-PP6M-5XUA-94B8 14560561 10.1309/XFUCPP6M5XUA94B8
de Cremoux P Coste J Sastre-Garau X Thioux M Bouillac C Labbe S et al Efficiency of the Hybrid Capture 2 HPV DNA test in cervical cancer screening. A
Poonam Yadav, Hima B. Musunuru, Jacob S. Witt, Michael Bassetti, John Bayouth and Andrew M. Baschnagel
helical tomotherapy and fixed field IMRT: plan quality, delivery efficiency and accuracy. Med Phys 2010; 37: 1350-9. doi: 10.1118/1.3326965 Rao M Yang W Chen F Sheng K Ye J Mehta V et al Comparison of Elekta VMAT with helical tomotherapy and fixed field IMRT: plan quality, delivery efficiency and accuracy Med Phys 2010 37 1350 9 10.1118/1.3326965
27 Matuszak MM, Yan D, Grills I, Martinez A. Clinical applications of volumetric modulated arc therapy. Int J Radiat Oncol Biol Phys 2010; 77: 608-16. doi: 10.1016/j.ijrobp.2009
Vincenza Granata, Roberta Fusco, Sergio Venanzio Setola, Elisabetta de Lutio di Castelguidone, Luigi Camera, Salvatore Tafuto, Antonio Avallone, Andrea Belli, Paola Incollingo, Raffaele Palaia, Francesco Izzo and Antonella Petrillo
tumor biology. 60 DCE-MRI should be used to assess microvascular structures. 61 , 62 The DCE-MRI can be assessed semi-quantitatively or quantitatively. Bol et al . 63 evaluated the role of DCE-MRI to assess the therapy in a murine model, showing that DCE-MRI-derived parameters predict peptide uptake better than the “contrast amount-related” parameters. Consequently, DCE-MRI elucidates the correlation between vascular characteristics, peptide delivery and therapy efficacy, and may predict targeting efficiency. 63 Huh et al . 64 tested, in a clinical study, DCE
Primoz Petric, Robert Hudej, Peter Rogelj, Mateja Blas, Barbara Segedin, Helena Logar and Johannes Dimopoulos
-spin-echo sequence with high sampling efficiency (SPACE) for high spatial resolution imaging: initial clinical experiences. Invest Radiol 2005; 40 : 754-60.
Fütterer JJ, Yakar D, Strijk SP, Barentsz JO. Preoperative 3T MR imaging of rectal cancer: local staging accuracy using a two-dimensional and three-dimensional T2-weighted turbo spin echo sequence. Eur J Radiol 2008; 65 : 66-71.
Kim H, Lim JS, Choi JY, Park J, Chung YE, Kim MJ, et al. Rectal cancer: comparison of accuracy of local-regional staging with two- and three
Elham Raeisi, Seyed Aghamiri, Azin Bandi, Negar Rahmatpour, Seyed Firoozabadi, Sedigheh Kafi-Abad and Lluis Mir
The antitumor efficiency of combined electrochemotherapy and single dose irradiation on a breast cancer tumor model
Background. The aim of this study was to investigate the antitumor effectiveness of electrochemotherapy with cis-platin combined with suboptimal radiotherapy doses. Tumor radiosensitization was evaluated on large invasive ductal carcinoma tumors in Balb/C mice.
Materials and methods. Tumors of an average volume of 630 mm3 were treated with cisplatin, electric pulses, radiotherapy, electrochemotherapy, alone as well as in appropriate combinations. Tumors were irradiated with Cobalt-60 γ-rays at doses 3 Gy and 5 Gy in combination with electrochemotherapy using cisplatin. Controls included each of the treatments alone as well as the combination of the radiotherapy with electric pulses alone or with cisplatin alone. Antitumor effectiveness was evaluated by tumor growth delay, tumor-doubling time, inhibition ratio and the objective response rates.
Results. As anticipated, electrochemotherapy was more effective than the treatment with cisplatin alone or the application of the electric pulses alone. When treatments were combined with tumor irradiation at either 3 or 5 Gy, the combination with electrochemotherapy was more effective: at 5 Gy, 2 animals out of 8 were in complete remission 100 days later. In general the higher 5 Gy dose of γ-radiation was more effective than the lower one of 3 Gy.
Conclusions. The results of our study demonstrate that irradiation doses, 3 Gy or 5 Gy, increase the antitumor effectiveness of electrochemotherapy with cisplatin on invasive ductal carcinoma tumors. Good antitumor results were achieved in experimental tumors with a size comparable to clinical lesions, demonstrating that this three-modality combined treatment is useful for the treatment of large lesions even at sub-optimal radiotherapy doses.
Xin Li, Ping Liang, Jie Yu, Xiao-Ling Yu, Fang-Yi Liu, Zhi-Gang Cheng and Zhi-Yu Han
Background. The aim of the study was to evaluate the efficiency and feasibility of contrast-enhanced ultrasound (CEUS) with Sonovue in assessing of renal cell carcinomas (RCCs) following ultrasound (US)-guided percutaneous microwave ablation (MWA).
Patients and methods. Seventy-nine patients (60 males and 19 females) with 83 lesions (mean size 3.2±1.6 cm) were treated by US-guided percutaneous MWA. The CEUS results of the third day after the ablation were compared with the synchronous contrast-enhanced computed tomography (CT)/magnetic resonance imaging (MRI) results and biopsy pathological results. The follow-up was performed by CEUS and CT/MRI after 1, 3, 6 months and every 6 months subsequently. The combination of clinical follow-up results and CT/MRI imaging findings was the reference standard of CEUS results for evaluating the therapeutic effect. The identification of residual or recurrence tumour was assessed by two blinded radiologists.
Results. On the third day after MWA, CEUS showed 68 of 83 lesions (68/83, 81.9%) successfully ablated and 15 of 83 (18.1%) with residual tumours. Among residual tumours, 13 (86.7%) were confirmed by contrast-enhanced CT/MRI findings and biopsy results. The sensitivity, specificity, accuracy, positive and negative predictive value of CEUS evaluating the short-term MWA effectiveness were 100%, 97.1%, 97.6%, 86.7% and 100%, respectively. During the six years follow-up (median 26 months), the CEUS showed recurrence in 7 patients, and six of them achieved consistent results on CEUS and CT/MRI imaging. The sensitivity, specificity, accuracy, positive and negative predictive value for CEUS evaluating long-term MWA effectiveness were 85.7%, 98.7%, 97.6%, 85.7% and 98.7%, respectively.
Conclusions. The post-procedural CEUS demonstrated as an effective and feasible method in evaluating a therapeutic effect of RCCs following MWA.
Matej Rebersek, Masa Kanduser and Damijan Miklavcic
Gene Med 2009; 11: 1125-37.16.
Pavlin D, Cemazar M, Coer A, Sersa G, Pogacnik A, Tozon N. Electrogene therapy with interleukin-12 in canine mast cell tumors. Radiol Oncol 2011; 45: 30-9.
Cemazar M, Golzio M, Sersa G, Rols MP, Teissie J. Electrically-assisted nucleic acids delivery to tissues in vivo: Where do we stand? Curr Pharm Des 2006; 12: 3817-25.
Cao F, Xie XY, Gollan T, Zhao L, Narsinh K, Lee RJ, Wu JC. Comparison of gene-transfer efficiency in human embryonic stem cells
Numerical study of the electroporation pulse shape effect on molecular uptake of biological cells
Background. In order to reduce the side-effects of chemotherapy, combined chemotherapy-electroporation (electrochemotherapy) has been suggested. Electroporation, application of appropriate electric pulses to biological cells, can significantly enhance molecular uptake of cells due to formation of transient pores in the cell membrane. It was experimentally demonstrated that the efficiency of electroporation is under the control of electric pulse parameters. However, the theoretical basis for these experimental results is not fully explained. In order to predict the outcome of experiments and optimize the efficiency of electroporation before each treatment, we developed a model to investigate the effect of pulse shape on efficiency of electroporation.
Results. Our model is based on a developed chemical-kinetics scheme and trapezium barrier model, while selfconsistency was taken into account. This model is further supplemented with a molecular transport model to acquire the molecular uptake of cells. The investigated pulse shapes in this study were unipolar rectangular pulses with different rise and fall times, triangular, sinusoidal and bipolar rectangular pulses and also sinusoidal modulated unipolar pulses with different percentages of modulation. The obtained results from our modelling and simulations are in good agreement with previously published experimental results.
Conclusions. We therefore conclude that this model can be used to predict the effects of arbitrarily shaped electroporation pulses on cell membrane conductivity and molecular transport across the cell membrane.