Peter Manko, Lenka Demková, Martina Kohútová and Jozef Oboňa
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) Digestive efficiency of indigenous and invasive avian species fed fruit of invasive alien plants in South Africa. African Zoology, 50(4), 293–297.
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van Heezik, Y., & Adams, A. L. (2016) Vulnerability of native and exotic urban birds to housing densification and changing gardening and landscaping trends. Urban Ecosystems, 19(4), 1551–1563.
Washburn, B. E., Bernhardt, G. E., Kutschbach-Brohl, L
Roxana Elena Nemescu, Ramona Gabriela Ursu, Carmen Mihaela Dorobăț and Luminița Smaranda Iancu
Meningococcal infection requires a fast and accurate diagnostic method in order to correctly initiate the antibiotic therapy. The aim of our study was to assess the efficiency of Real Time PCR -Taq Man using sod C gene / N. meningitidis in comparison with the classical methods for the diagnosis of meningococcal infection - direct microscopy, cultivation, latex agglutination and blood culture. We have detected 24/44 (54.54%) patients with meningococcal infection. In both cases of patients with / without previous antibiotic therapy before admission, the AUC (area under curve) had the highest values for RT PCR in CSF and blood analysis. This sod C RT-PCR assay is a highly sensitive and specific method for detection of Neisseria meningitis and it would be useful to include this method like a multiplex in routine testing of patients with clinical meningococcal infection for other etiological agents also.
Monika Rawat, Kusum Arunachalam and Ayyandar Arunachalam
Background: The primary function of the leaf is the production of the food and interchange the gases between the atmosphere and the plant surface. Establishing the relationship among the leaf traits is essential to understand the ecosystem functioning in the forest ecosystem. Here, the present study proposes a framework for species-level relationships between the traits in the temperate forest ecosystem. Methodology: Three morphological (leaf area, specific leaf area and leaf dry matter content), three chemical (leaf carbon, nitrogen and phosphorous content) and six physiological (chlorophyll, photosynthetic rate, stomatal conductance, intrinsic water use efficiency, transpiration rate, intercellular CO2 concentration) leaf traits were analysed in 10 woody tree species of temperate forest using linear mixed modelling. Results: Results showed that the leaf carbon was the only trait influencing the most to leaf area, specific leaf area and leaf dry matter content and leads to maximum variation in the functioning of the forest ecosystem. Conclusion: The results suggested that consideration of plant traits, and especially the leaf traits, increases the ability to describe variation in the functioning of the forest ecosystem. This study indicated that leaf carbon act as the significant predictor of leaf trait variation among the different species in the temperate forest ecosystem of the Indian Himalayan region.
Cristina Rusu, Adriana Sireteanu, Lăcrămioara Butnariu, Monica Pânzaru, Elena Braha, Doina Mihăilă and Roxana Popescu
Duchenne and Becker muscular dystrophies (DMD/BMD) are X-linked progressive muscle disorders determined by mutations of the dystrophin (DMD) gene. Multiplex Ligation - Dependent Probe Amplification (MLPA) is a simple, inexpensive and reliable test for molecular diagnosis of DMD gene mutations. It identifies exonic copy number variations in the DMD gene, but the test should be completed with sequencing analysis in case of single exon deletions/duplications. The aim of this study was to evaluate the efficiency of MLPA as a DMD mutation screening tool in affected males and carrier females, as well as to appreciate the frequency of different types of mutations and to check the validity of the “reading frame rule”. We have used MLPA for the detection of deletions/ duplications in DMD gene in 53 individuals (30 affected males and 23 asymptomatic female relatives) referred for evaluation and genetic counseling due to the clinical suspicion of DMD/BMD. In the affected males (21 DMD and 9 BMD) MLPA had a detection rate of 63.5% (53.5% deletions and 10% duplications). The most frequently deleted exon was exon 45 and the most frequent duplication involved exons 3-5, confirming the presence of the two hotspot mutation regions reported in the literature. Mutations detected in our study have a slightly different location compared to literature data. Reading frame rule was valid in 84% of our cases.
Cătălin Carp-Cărare, Alina Vlad-Sabie and Viorel-Cezar Floriştean
Listeria monocytogenes represents an important hazard to human health because it is capable of causing listeriosis, an atypical foodborne disease with a high fatality rate.The aim of this study was to detect and serotype Listeria monocytogenes in some food products (of animal and vegetable origin), collected during the 2011 period. The detection of Listeria monocytogenes was performed by ISO 11290- 1-A1/2004 standard method and verified by real-time PCR. Numerous subtyping strategies have been developed to characterize Listeria monocytogenes isolates. Serotyping, the traditional method for the subtype characterization of Listeria monocytogenes is not very discriminatory, but it is a universal method which gives important information on prevalence of specific serotypes. The confirmation of Listeria monocytogenes isolated strains was performed by a TaqMan real-time PCR assay, using TaqMan Pathogen Detection Kits (Applied Biosystems). The assay use specific primers and a probe for Listeria monocytogenes. In this study on a total number of 260 samples, 25 (9.61%) gave positive results for Listeria monocytogenes by conventional method and only 22 (8.46%) of them were confirmed by real-time PCR. These results indicate a high efficiency of the real-time PCR method in a short time, compared with classical methods which can provide false positive results. Four different Listeria monocytogenes serotypes were found in nine varieties of food products: 1/2a, 1/2b, 1/2c and 4b
Aylin Uskudar-Guclu, Mustafa Guney, Ali Korhan Sig, Selcuk Kilic and Mehmet Baysallar
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Laura Pindaru, Iulia Maria Balaci and Ioan Ştefan Groza
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Giovanni Amori, Valentina De Silvestro, Paolo Ciucci and Luca Luiselli
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Dumitru Tudor Zdrenghea, Maria Ilea, Mihnea D.T. Zdrenghea, Adela Viviana Sitar-Tǎut and Dana Pop
25. Guazzi M, Vitelli A, Arena R. The effect of exercise training on plasma NT-pro-BNP levels and its correlation with improved exercise ventilatory efficiency in patients with heart failure. Int J Cardiol. 2012;158(2):290-1. DOI: 10.1016/j.ijcard.2012.04.091
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