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, Qiua S, Zhoua W. Establishing a scientific basis for fertilizer recommendations for wheat in China: Yield response and agronomic efficiency. Field Crop Res. 2013;140:1-8. 10. Commission Regulation (EC) No. 889/2008 of 5 September 2008 laying down detailed rules for the implementation of Council Regulation (EC) No 834/2007 on organic production and labelling of organic products with regard to organic production, labelling and control. Official Journal of the European Union. 2008;L 250:1-84. 11. Connor DJ. Organic agriculture cannot feed the world. Field Crops Res

REFERENCES ALLEN R.G., PEREIRA L.S., RAES D., SMITH M.: Crop evapotranspiration: Guidelines for computing crop water requirements. Irrigation and Drainage Paper 56, FAO, Rome, Italy, 1998. AL-JAMAL M.S., BALL S., SAMMIS T.W.: Comparison of sprinkler, trickle and furrow irrigation efficiencies for onion production. Agricultural Water Management, 46:253-266, 2001. BASTOS E.A., SILVA C.R., RODRIGUES B.H.N., ANDRADE J.R., IBIAPINA L.M.M.: Evapotranspiration and crop coefficient of drip irrigated watermelon in Piaui Coastline, Brazil. Engenharia Agricola, 32


There is a growing need for new formulations of carriers with better protection for bacterial inoculum. One of the newer techniques in inoculum making is encapsulation method. With this method, the whole bacterial cells are immobilized in defined space – matrix, where the cells are protected from environmental activities before use. Encapsulation of the inoculum was performed with ionic gelation method. The alginate-based microparticles (500-600 µm) containing viable B. japonicum strain were solidified in CaCl2. The initial number of viable bacteria in every sample was 9.0 log CFU/ml. Chitosan coated particles had a higher mortality rate than non-coated particles, with 1.3 log CFU/ml in lyophilized and wet microparticles stored at room temperature. High viability of B. japonicum was registered in wet particles stored at constant −20°C for thirty days with a viability rate of 8.84 log CFU/ml.

REFERENCES AL-JAMAL M.S., BALL S., SAMMIS T.W.: Comparison of sprinkler, trickle and furrow irrigation efficiencies for onion production. Agricultural Water Management, 46: 253-266, 2001. AYARS J.E., PHENE C.J, HUTMACHER R.B, DAVIS K.R, SHONEMAN R.A, VAIL S.S, MEAD R.M.: Subsurface drip irrigation of row crops: A review of 15 years of research at the Water Management Research Laboratory. Agricultural Water Management, 42:1-27, 1999. BARTOLO M.E.: Subsurface drip irrigation in Colorado. In: Central Plains Irrigation Conference and Exposition Proceedings, Sterling


Young plants of celery, parsley, parsnip and carrot, grown in nutrient solution, were treated with sodium naphthenate (10−7 mol dm−3), applying foliar and root treatments. Both treatments affected the root content of all investigated elements present in the nutrient solution, but in a different way, depending on the plant species. An average change (increase/decrease) in the contents of investigated essential elements was about 35%. Our experiments with naphthenate showed that this treatment may enhance the efficiency of essential elements uptake and increase its content in plants without changing concentration of these elements in the nutrient solution. Especially interesting results were obtained in the case of carrot, as increased contents were observed in the elements that are usually deficient in nutrition (Fe, Zn, Mn), whereas the other remained unchanged.


Gastro-intestinal nematodes (GIN) of sheep are one of major constraints in grazing production systems worldwide. Control is commonly achieved using anthelmintics, but global occurrence of anthelmintic resistance to different drugs and the emergence of multi-resistant GIN species seriously limit the efficiency of their use. Therefore, integrated parasite management is widely recommended, with nematophagous fungi as one of control tools. Duddingtonia flagrans is one of the most used species, with various effect of different isolates. In previously performed coproculture assay, we showed low efficacy of D. flagrans MUCL 9827 against infective larvae (L3) of sheep GIN. The aim of current experiment was to reevaluate its nematophagous potential, using the medium where direct interaction between the fungus and L3 could be observed. Nematophagous activity was tested on 2% water agar with addition of chloramphenicol on three series of plates seeded with 500 and 1000 chlamidospores and agar blocks with 7 days old mycelium. At Days 0 and 5, 500 L3 of sheep GIN were added to test the trapping activity. The cultures, including control plates with only L3, were incubated at 25°C for 10 days, followed by evaluation of their number and reduction percentage. Nematophagous activity of D. flagrans MUCL 9827 against L3 was clearly demonstrated. However, the overall efficacy was poor since trapping was observed only in one out of nine plates containing fungal material. Potential reasons for such poor performance of the isolate of fungal species, otherwise known as successful in trapping animal parasitic nematodes, are discussed.


This study assessed the potential efficiency of selected biologically active substances on the motility behavior of rabbit spermatozoa subjected to in vitro induced E. faecalis contamination. Semen samples were collected from 10 male rabbits and the presence of E. faecalis was confirmed using MALDI-TOF Mass Spectrometry. For the in vitro experiments rabbit spermatozoa were resuspended in the presence of 0,3 McF E. faecalis and different concentrations of selected biomolecules (resveratrol - RES, quercetin - QUE, curcumin - CUR, epicatechin - EPI, isoquercitrin - IZO). Sperm motility was assessed using the computer-aided sperm analysis at 0h, 2h, 4h, 6h and 8h. The presence of E. faecalis significantly decreased the motility (P<0.001) when compared to the untreated Control starting at 2h and maintaining this negative impact throughout the entire in vitro culture. Meanwhile, the motility was significantly higher in the experimental samples subjected to E. faecalis together 5 μmol/L RES (P<0.05), 10 μmol/L QUE (P<0.05) as well as 1 μmol/L (P<0.01) and 10 μmol/L CUR (P<0.05) when compared to the Positive Control (4h). No biomolecule was able to maintain the motion comparable to the Negative Control, and none was effective against the rapid decline of sperm motility caused by the presence of E. faecalis during later stages of the in vitro experiment (6h and 8h). We may conclude that RES, QUE and CUR may provide a selective advantage to spermatozoa in the presence of E. faecalis, particularly during short-term rabbit semen handling.

REFERENCES ARTECA RN: Introduction to horticultural science. 2 nd ed. Cengage Learning, Stamford, pp. 117-120, 2015. BASRA AS: Plant Grow Regulators in Agriculture and Horticuture. Their role and commercial uses. The Haworth press, pp. 1-16, 2000. GHASEMI Y, NEMATZADEH GA, OMRAN VG, DEHESTANI A, HOSSEINI S: The effects of explant type and phytohormones on African violet ( Saintpaulia ionantha H. Wendl.) micropropagation efficiency. Biharean Biologist, 6 (2) 73-76, 2012. GEORGE EF: Plant Propagation by Tissue Culture. Part II. In Practice, 2 nd ed, Exegetics Ltd

Pollutants Effect on Ornamental Plants Leaves by FT-IR Spectroscopy. Acta Physica Polonica, A., 129(1). Jones A.P. (1999): Indoor air quality and health. Atmos. Environ. 33: 4535–4564. Kays S.J. (2011): Phytoremediation of indoor air—Current state of the art. The value creation of plants for future urban agriculture. Nat. Inst. Hort. Herbal Science, RDA, Suwon, Korea, 3-21. Kim K.J., Kil M.J., Song J.S., Yoo E.H., Son K.C., Kays S.J. (2008): Efficiency of volatile formaldehyde removal by indoor plants: Contribution of aerial plant parts versus the rootzone. J. Amer. Soc

COUNCI Directive 96/61/EC Directive 2001/77/EC, Directive 2003/30/EC, Directive 2009/28/EC, Directive 2003/54/EC, Directive 2003/55/EC of the European parliament and of the Council, The Kyoto Protocol EUROSTAT 2017, web: FORGY EW. Cluster analysis of multivariate data: efficiency versus interpretability of classifications. Biometrics, Vol. 21: 768-769, 1965. GULAN B. Stočarstvo Srbije, Makroekonomija, 20.08. 2016. web: GUTZLER C, HELMING K