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In silico selection approach to develop DNA aptamers for a stem-like cell subpopulation of non-small lung cancer adenocarcinoma cell line A549

dissociation constant and the highest binding energy. In cancer, aptamers have been suggested to replace the antibodies mostly for diagnostic purposes, as they are more reliable in terms of reproducibility, stability, and costs of production 7 . The detection of circulating tumour cells (CTC) in body fluids prior to, or at the first medical intervention, would represent a particular challenge for the prediction of disease progression 8 . The two technologies used for CTC enumeration, the Cell Search System ® based on the detection of cancer cell membrane protein markers

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